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CRIMIC CR00517

Gene MFS3

CG4726

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:1145293..1145293	21F1	FBti0200576	80587

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	CCACCACGCACCACCTAATCAAACAAT	GAGGAACATATACCAGCCGAAGTTGC

Images

Construct Design Sequences

sgRNA sequence	AATGATATTCACATCGGACATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCGTTATCGCAAAGCAACGC	GTGCGCCAAATGACTCCATT	CGCACCATGTCCGATGTGAA	AATCAGGCCACCGTAATGCA

 

Amplicon 5'

Amplicon 3'

TCGTTATCGCAAAGCAACGCACTCATAATTGCTGGACATGATTGCCTCCCCCAACCGATTTCAAAAGGGGGTGGACTATATCAATGCGCGTGCGGCGGCTCACTCTACTGACCCTACCCAACTGCCGGTAATCAGTGGCTGCTCCATGTGGAACTCGTTGCTCACCAGGTGATTGGGTTCGGAGCACCCGAGTGGTGTCATGTCCCTATAAATACCTTAATGGTAATGTGTGTAAGGACTATCCCCACTGTCTTTAGATGTTTGCTCTTCCTTCCTTCAATTAAAAAAACAAACGAGAACGTTGTCGTAACGACTATTTGATACCCCTTACTCAGATAGCGATTCAATAACACGGACAAGCAGACGGACAGGGTCAGTTTGTTTCGACTAGTGTTTCCGATCAAGCCCATGTCACACATACACACGTTTCACACGAGTCTAGTATACCCTTCAGCACTTCAAGTAAGGGGTACAATTAATTCCTTAACTTGCTAAATGGAGTCATTTGGCGCAC

CGCACCATGTCCGATGTGAATATCATTGGCATTAATCAAATTGACTTGCCAAGCAAACCAACCGAATGACTTATATATCGAGTTGCATTTTCCATCCATCCAAAATGGACGAATCAAGTAAAAATAAAGATTTACAAAGACCTTACCATCTACTTTCCAGAACTCGTGCCCGCCCGCTATGTGCTGGCCCTCCTGGGGTCCATCGGCATGGCCATTGTGTACGGCCTGAAGGTGAATCTCAGCGTGGCCATGGTGGCCATGGTGAACCACACAGCCATTAAGGCCCACGGAGACGGCGGTGGACATGGGGGACACGGCAGCGTCATCCTTTCGAACGCCTCCCAAGTGTCCCTGGTGGAGGAATGCAACCCGCCGGGTGGTGCCTCCAATGTGACGGCTAAGGTGGAGGACGGTCCCTTCGACTGGAGCGAGCCCCTGCAGGGAACCCTGCTGAGCTGCTACTTCTGGGGCTATTTGGTCTCGCAGATTCCGCTGGCACACGTCGCCGAGAACTTCTCCGCCAAGTGGGTGATGCTCTTCTCGGTGGCCATCAACGTCGTGTGCACGCTGCTAACGCCCGTCTTTACTGAATTGCATTACGGTGGCCTGATT

 

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