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CRIMIC CR00521

Gene Npc1a

CG5722

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:10218474..10218486	31B1	FBti0199399	78937

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CTGCTTATCGAATTGCAGCGCATCTG	ACCTGTTCCAGCCTATAGAATGGCGA

Images

Construct Design Sequences

sgRNA sequence	AGCTAAGCTAAGTTGAAGTCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AACTCACCCATGGACGAACC	TTCAACTTAGCTTAGCTCACTCT	AGCTATCTTTTTGCTGACCCC	TGAATTTGCCAGGCTCGGAT

 

Amplicon 5'

Amplicon 3'

AACTCACCCATGGACGAACCTTGTGTAAACAGAAACGAGCCCATTAAGAATACTAAGACTCCCACCAAGAAGACAGCTGCCATGATGACGAAATAGGCGTCGAGTCCAACAATTTTGAAGGGCTCTGGACGGGGCGGTTCCGGCGGTCCCTGGGGACAGGATAGGTCGCAGTCCGAGCAGGAGCACGCAGGTAGTTTGCTCTACAAGCAATTTATATATTTAGCACAAAATGAAATGAATTTCCAATAAGGATAGTAGGTACATACGCTAACTGCTTGGTTGCAAGGAACGGTCGTTACATTGAGGGGCGTAAAGTTATTGCTATTTGATTTGGGCTCATGTTGAATATAGGTAATTTGAAAAGGAACATATGGATTGGTGGCATCGCCCATAAAGTTGAACCATTTTGTAGGATTGCAGCGAGATGCTGAGTAAGCGCCGCACATTAAATCAAAAGCTAATTGACCCGTTTGCGGTACAGAAACCTATTTGAACACAAACAATAATTATTTTCGATCAATGAATAATGTATATGGAATCCAGAAGTACCTGAGAGCACGATTTATAAGTCTTGTTGATGTATTCCGTTGATATATGCAAGTCAACAGAACTTATGTACTCATCACCTGAAATTCGGAATATATAATTATTCAATAATTAATAAGCTATTAATAATGATTACCTTTCTTATTTTTCTGCGTGGCCACCACATGCATGAACTCAGCCTGCTTTGGTGAACAGGTGAATTGGCAGATGTGCCGCACCAAGTTCTCCATGCAGGATGGACATCGATCCAAGATATTTCCGGCCAGCTCCACATTCTTATTAAGGAGCTCCACCTGCAAAGGCATTTGTTTGATAATTATGACGCCTGGGCAAAGCTAATGCTACTCATAACCTGATTCTTATCGCAGCAAAATTTGTTTTCGCTATTCTCCAGCAGAAATCCACAGCGTTTCTTCAGCAGCTCCAGACCGTCAGTTGCCATTTCCTTCGCTGTTCCATTGTACGGACAGTTTTGGCTGTGCGAAAAGTCATTGGTGTTGCACACGCCATACCAAACGCAGTCCTTAAAAAGTTGCAAAATAAGTTAATAACATTATAGAGCAGAGATATGTAGAGTGAGCTAAGCTAAGTTGAA

AGCTATCTTTTTGCTGACCCCAAATACCTTTTTCAAATTTAAATATTACTTCCAAAACTAAGTACGTCATCTCACCGCCACCCAGAGCTATAAAAATATATATTTTTTCTAATTGCCTTGCATCTGTTTTAGCTCATTCATTGTTGAGGTGAAACAACAGCTTTTCCAATACAATTTACCAGTCCATTTTCAGAAATGATTTTACGACGACTAATCTGCGCGATTAAAAACCCAGTATGCTTAATATTGATAAAATATTGAAATTTAATGATAAAAACCAAATGTTAGTTTACCTGTTTCGATGATTGAATTAGCGTAAAGAGCACAGCAGCGATGAGGATGTGTACTCCAAATGGAGATATCCTGAGCGGGGATCTTGGACTCATTCTATCACAATCGCATATCTGTAAAGAACGTTTTTGGTTAAAGTATGCACCTGGGCTTACTATTATGTATGATATTCGAAGTAAGAGTCTCGGTTTTCGACTAAGAACTGAATCAAATGTAATCCGAGCCTGGCAAATTCA

 

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