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CRIMIC CR00523

Gene Oct-TyrR

CG7485

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:22061859..22061859	79B2	FBti0200578	80588

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	GATAGCCTTGTGCGGTAACTTTGTCC	TACCTGTATGCAACCTTTGGGATCGC

Images

Construct Design Sequences

sgRNA sequence	ACTACCAGTTTGGTGGTCTAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCATGTAAACGCAACCGGAG	AGGGTCACTTGCACTACGTG	ACCCTCCCTAGACCACCAAA	CTGGTGGGTTTCACTGCTGA

 

Amplicon 5'

Amplicon 3'

GCATGTAAACGCAACCGGAGCCCAAAGAAGGCAAGTGGCAGGGCAGGGAAAGATGCCATCGGCAGATCAGATCCTGTTTGTAAATGTCACCACAACGGTGGCGGCGGCGGCTCTAACCGCTGCGGCCGCCGTCAGCACCACAAAGTCCGGAAGCGGCAACGCCGCACGGGGCTACACGGATTCGGATGACGATGCGGGCATGGGAACGGAGGCGGTGGCTAACATATCCGGCTCGCTGGTGGAGGGCCTGACCACCGTTACCGCGGCATTGAGTACGGCTCAGGCGGACAAGGACTCAGCGGGAGAATGCGAAGGAGCTGTGGAGGAGCTGCATGCCAGCATCCTGGGCCTCCAGCTGGCTGTGCCGGAGTGGGAGGTAATAAGCACAATGATAGTATTTCATTTCATTACTAATAATAACGGATAGCTTCTGCCTTTTTGTTTTTTATTAACGTTTGACAGCAAAAATAAAATATTCACATGCAAACCTTACCTTTTAAAAAATTGCGTTATAAATCCCCTTTTACTTTTGACCATTATTTTGATCAAGTATTTGTATACCCAACCGCCTGTTTGTTCGTACTTCTGTCTGTCCGTCCGTATATCTGTCCGTATGAACAGAAACTATAAAAGAATGTACAACAGGAAGTTGAGATTAAGCATATTGGTTTTAGTTAACTTTTACGCAGTGCAACCAAAATTTAAAAAAATGTTATAATTTTAATAGATTCTATCATTTGTCTTGCCAATTTCTATTGAAAGTTCGCCTGCCACAAATTTCACTTATATTAAGTGTGACCGTCACCACGTAGTGCAAGTGACCCT

ACCCTCCCTAGACCACCAAACTGGTAGTTTCTACTCTTTCAATAAGTCTAAATACCAGTAGATCTTATTTATGTTGGTTTAAAGCTCTCCCTAACTTCTTTTCTATTTTAACTAGGCCCTTCTCACCGCCCTGGTTCTCTCGGTCATTATCGTGCTGACCATCATCGGGAACATCCTGGTGATTCTGAGTGTGTTCACCTACAAGCCGCTGCGCATCGTCCAGAACTTCTTCATAGTTTCGCTGGCGGTGGCCGATCTCACGGTGGCCCTTCTGGTGCTGCCCTTCAACGTGGCTTACTCGATCCTGGGGCGCTGGGAGTTCGGCATCCACCTGTGCAAGCTGTGGCTCACCTGCGACGTGCTGTGCTGCACTAGCTCCATCCTGAACCTGTGTGCCATAGCCCTCGACCGGTACTGGGCCATTACGGACCCCATCAACTATGCCCAGAAGAGGACCGTTGGTCGCGTCCTGCTCCTCATCTCCGGGGTGTGGCTACTTTCGCTGCTGATAAGTAGTCCGCCGTTGATCGGCTGGAACGACTGGCCGGACGAGTTCACAAGCGCCACGCCCTGCGAGCTGACCTCGCAGCGAGGCTACGTGATCTACTCCTCGCTGGGCTCCTTCTTTATTCCGCTGGCCATCATGACGATCGTCTACATCGAGATCTTCGTGGCCACGCGGCGCCGCCTAAGGGAGCGAGCCAGGGCCAACAAGCTTAACACGATCGCTCTGAAGTCCACTGAGCTCGAGCCGATGGCAAACTCCTCGCCCGTCGCCGCCTCCAACTCCGGCTCCAAGTCGCGTCTCCTAGCCAGCTGGCTTTGCTGCGGCCGGGATCGGGCCCAGTTCGCCACGCCTATGATCCAGAACGACCAGGAGAGCATCAGCAGTGAAACCCACCAG

 

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