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CRIMIC CR00526

Gene CG6012

CG6012

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:16860858..16860868	36B2	FBti0199401	78939

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGGAGGCGTGTGTGTACTTAGCATTT	CTAGCGAAGCTTCAAACCAGTTGTCG

Images

Construct Design Sequences

sgRNA sequence	TATGATTGAAAGCCATGCAATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGCCGATGGGATTAGGAGAC	CGGAAGTATATGCCGATTGACA	CCATTGCATGGCTTTCAATCA	CCTCGCCAACACGACTTTTC

 

Amplicon 5'

Amplicon 3'

CGCCGATGGGATTAGGAGACCACCCTTCATGATCTGCCTGGAGTACGAGTTGATTTTGGTAACCACAAAGTTCGGGGACAACATCTGCACATGGATGCCATAAGGCTTCGCCTCGTGGTACAAGGCCAGGGTTAAACTACGGGTGTACGCCTTGGAGGCGGCATAGTAGGCTCCATTGGGAAGTGGCTGCAGTTCCGTGCCGGAACCAACATTCACAATGGCTCCCTTCAATTTAGACGCCTTCATGCGCTGGAAGAAGATGCGAGACAGCTGGGAAACGGCCACCACGTTGGTGTCGATGATGTTTTGCGTTTCTTCCTGGTTATATTTCAGCAAGGATTTCGGCGTGGCGATTCCAACGTTGTTTACTGCGATAAGGTGAAATTGTTTATGCAAGTGAAATAAATACCACTTACATAAGTAAATGCTACTTACCAAGTATGGAAATGGGTATATTGGCAGTTTCCTTTTCTATGTGCTCATAGACCTGCGAGCCCTTGGTAAAATCAGCTATCACTATTTTGGTCTGAACACCGGCACCGCAATCCGCTAAAAACAAACAAAATGAATCGAAAAAAATGTATGTTACCAAAATTAGATGTCAATCGGCATATACTTCCG

CCATTGCATGGCTTTCAATCATAATTTCGACAAGTGCTTTGCGTGTTCTTTAAAAATGTCAGTCTGTTGTAACGGAAACAATCCAAACTACAGTCTTTAAATGCAGTTAACCAAAATTTAAAGCTATTGAACTTGTTTGTTTAATTACTGGTATTTACTGGTATGGTAAGATTACATAACGATTAACAACTGTATTATCTTTTAACGATTATACAAAATAACTGTTGATAAGTCGTATTTGGCATTTTTAATCTCTCCACACCTTGACTTACCGATTTCCTTGGCAACCGCCTGGAGTTTCTCCTCGGTTCTAGCGATTAGGACCACATTGATGTTCTGGCGGGCCAGCTCCTTGGCATATTCCTTGCCAATTCCATCGCTGGCACCCGTGACAGCGGCCCAATCTCCGAAACGCTCCTTCAGAGTTGGCCTAGTCCCACTGAAATACCTGATTTTGATTAATTTGTACGGCGTTCTTAACTGCTCGTAGAGATAAGAGGACAGGGCATAGACACCAACGAATGTGAGGAATGCTGATAGGGCACAGAACATTTTGTCGATTTATTTAGTAATTTTAATCGACTGCGCTACAGCGGAAAGTACGTGTGTGTTATACGGTCGACACACTTGAACGGACTGTAAAAAAATTGTAAGCAAAATTGCACAGTATTCGCCGGGCAGGGCGCTGAATTTAAATGGCTTGAATTAATAGAACTCGGTGCTATTAAAAGGTGTTCGACTTGCCGTTAAAAATAGCCCGAAAAATTGTATTCATAATACAACTTTGCCTATTCAATTATGGTTTGCCTAATGGTTTGACTTGCTGTTGGCCTGGAAATGGTCAAATTAAATAGGCACATCCATTAACAAATATGATTGCATTACACTGGAAATGTGTGAGAAAAGTCGTGTTGGCGAGG

 

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