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CRIMIC CR00529

Gene Cyp4e2

CG2060

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:8445037..8445047	44D1	FBti0199602	79275

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	CGATTAGTCACTAGGTGGAATGCCGT	CGGAATTACCCATTACTTCGCGTTGC

Images

Construct Design Sequences

sgRNA sequence	TGCGCATCGAATCTACTCAGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AACTCAAAACGTGACGGAGC	TCGATGCGCAGTTACCTCAG	GCGGCGAGGCTATTCAAGTA	TCCTGCTATAGGCCGGGTAG

 

Amplicon 5'

Amplicon 3'

AACTCAAAACGTGACGGAGCGGAACAGAATAATCGAAAAATAAACGCACACTGTGCTGATATCATTTCTTAAATGTAAATGTAAATTTAAATTTGCAGTGGATTTTACCTTACGTCTGTGATTTCTACCAAATTTAAGTCTTGCTGCTGATAAGCTAAGAAGAGCAAGCGACTTTCTCTCTCTTCTCTTCAATTTCTGTAAGAGTAATCTTAAAAACATTTTGCAAACATAATGTGTTCATCAATTCTCCCGATTTCTCTTTCTTGTTGTGAGCGAATGTTTATCAACGTTTTGTTTTATTCCATTTTTTGCTGCCATAAAACAATATATACTGGATACAGTTTTAAAAAAAGTTAACTGTTACACATTTAAGCGCAGTCTCGAAAACTATTGGTTCTCCATCTCATCTAACGATTAATCAGGGGGCTCATCTTAAAAAACCTGATATTGATTCGGCAAGTCATGCTGAAAGCACCAAAATATATACTTATATTCGGGAGACTACAGTTGGATTAGGAAAACGGCAGATAAGGGAGCAAGCGACTGGGGAGCTGGCTGATATTGGTATCTCTCGTATTTAAACCGCCCGCCGGCCCAAATAAGCTCTCATATTTCCAGTGATTCGCGGGCTGGACGGTTGTCGTGGAACTCGCGATCGAAAATGTGGTTCGTGTTGTATATTTTTCTGGCCCTACCACTGCTGCTGGTTGCATATCTGGAGTTGAGCACATTCCGCCGGAGAAGAGTGCTTAACAAGTTCAACGGCCCCCGCGGCCTGCCGCTGATGGGAAACGCTCATCAAATGGGCAAGAATCCGTCAGGTGGGTTTTTCATAGGAGCCGGAAAAGTCGTAGCGTTAGCCACGCAGAGGACACACCTGAAATTACCCATCTCGAATAGCGTTGTGACTGAGGTAACTGCGCATCGA

GCGGCGAGGCTATTCAAGTAAGGTTATTTTGGGCCAGCGACTTTACGACCGGAAAACAAGGGTGTTAACTATCAGATAATGGGGCTACTGGCACTTGTAGAAATGGGCTAATCGTAGCCTAGGTCGAGTATCGCAAAAATAATGACGAGGCTTTGATTTGTGTTGTGTAATATACGATGTATGTATGTATGTATTATACTGCACTAATGAATACTGAAAATCGCGTGCCAAAAACAAGCAATGATTATCTACGGAATACCGATATTGGTACGCGATCCGCCTACTAATGGCATTTTCTCACGTCTATATAGAAATACTCGACACGGTTTTCTCCTGGTGGCATCAGTATGGAAAAGACAACTTCGTCTTTTGGATTGGAACGTATTCTAATGTATTGGTCACCAGCTCCAAGTACCTTGAAGTGAGTTACAGCGAATTTCCACCAATCATGTAATCATGTGACTCTATTATTTTCGTACTTCTTCAGTTCATACTCAGCAGTCAGACGCTGATCACAAAGTCCGACATTTATCAACTAACACACCCTTGGCTGGGATTAGGTCTCCTCACCAGTACTGGCAGCAAATGGCATAAACACCGCAAGATGATCACCCCAGCATTCCACTTCAACATCCTGCAGGACTTCCACGAGGTGATGAACGAGAACTCCACCAAGTTTATCAAGCACTTAAAGACAGTTGCTGCCGGGGACAATATATTCGACTTCCAAGAACAGGCCCACTATTTGACCCTGGATGTCATTTGCGACACAGCGATGGGTGTGTCCATCAATGCCATGGAAAACCGCAGCTCTTCCATTGTGCAGGCCTTCAAAGAGTTAGTTTCGTACCTCGTAGTCGAACTATCAGATTGCATGACTCTTGATCCTTTTCAGCATGTGCTACAACATCAACATGAGAGCCTTCCACCCGCTGAAGCGCAATGAACTGCTGTATCGTTTGGCTCCGGACTACCCGGCCTATAGCAGGA

 

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