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CRIMIC CR00530

Gene CG3078

CG3078

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:2257691..2257691	2F2	FBti0199403	78941

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CACTAATAGCAGCGGCTAATAGGCAC	TCGCCGTGCTCGTTCCTATTGTAGAA

Images

Construct Design Sequences

sgRNA sequence	AAGGGGTGCCCTGTGGTCATTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATAGGCGGGTAGAAGGGTGT	TGGTGTGTCGTGAAAGGTGA	ACACACCAATGACCACAGGG	GACACAGCCTTATACGCGGA

 

Amplicon 5'

Amplicon 3'

ATAGGCGGGTAGAAGGGTGTAAATGGAGGCGTACAGGTGCGACAGAAGAAATAGGAGCACTGTCATATAGTTTGCCACAATTAGTGGCCCATAACCATATCGTTTCATCAGTCGAATGCTGGGCAGGCTCATACCAGCGGCGACCAGGAAGAGAGCACTCAGCAGAAGGGATCCAACCTCCGGACCGAGGGGCAACCATTCCTCCCGATGGTGCCACACCGCATTGGAGCCCTGGAGCGCCATCACCGGCAGCAGAACGCAGTTCATCAGTCCGTGGGCCAAGCTTAAACAGCTAAACTGGCGCCACACTTTTTGGCGCACATGGGCTGGCACCTTGTGCCGGCAATTCCTGTTGTTCAGCGTTATCAATCGCCGTATGGAGGTGGCTCCAATGGAGCTGTTTATGGACTCCCGACGACGAGAGGCCGCCAGAGCAGCCTGCACCTGGTACGAGGACACCGGATTTATGCGATGCGACCTCGTCGAAATGGGTGACGGCGAGTATGTGGTGAAGTGCTGCTGGAAAAGATGCAAGAAAAGGGTATTAGGATATAGCAAGAATCCAATCATCTGGCAGGCTAATGGTATTACAGCCAGCTACCTTTCACTTTAACAATAGCAAAGGGGGAGTAGTCGGAGTAGGGAAGTAGTTGTAGTTCAACTTGGAAAGAGAGTCCGTGTCCGAACACTCACCTTTCACGACACACCA

ACACACCAATGACCACAGGGCACCCCTTTTGTGCACACATCGCCACATGATAGTTGAGTGTCCAGGAAAAAAGGGAGATAAGCTCCCAGATTCTATTTATATGCGACAGGTGTCACCATGCCGGAAAGCAAGTGACATGAATTATATAAAGGCTTTTCACACAGAGGCGGACTTTTTATGTGTTCACCTGAGAACTCACCTCCATCATCTGCTCTTCCCAGACGTTCTGGGCTCCTCCTGAACTGCGGGTTCGAAGCACTCTGTGCCTGGTGGTCAGCGAATGGCGGTGCTGATGGTGCTGGTGCTGGTGCTGATGCTGGTGCTGATGCTGATGGTGGTGTGAGTGTGGGGCATACTTCTTGCCCAGTCCCAGCATCGCAAAGCCACTGGTGGCCACCGGAGCTCCCGAGCTGGTGGCCAATCCAGAAGCTGGGCCCACTGCCTGGTTCGGACCCTTTCCGCCAGTTGTCCCATTGCCACCGAGCAGCTCTCCGTTGCCTGTTGCAGTTCCTGAGGTGGCATTGAGGCTCAAGGATCCGCCACCAATCCCCAGACCAGCCATTATGTAAGCATTATACGACGATTGCTGAGTGGAGTAGAAAAATACATAAGTCGTAAGGAGGGGGGATCTACTCAGGCGATCTCACCTTGCGCTGCTGAGCAAACGCGGAGAGACGCTCCCGCTCGCGACTGGTGTCCCGAGCCCGCTGCTCCAGCAGAAGTTCGTACTCCCGTCGCTTCTCCCGCTTGCGCTCCGCCTCCTCCAGCTCGTGAAGATTCGGCAGACTGCCCATTTCCGGCGATTATTGCCAACTTATCAAAACCTAGCGCCCGTAGTGCGAGTTATGTGGATTGTGGGGCCACTGCGAATCCAGTACTGCCATCGCTCCAAAACGGCGAACTCATGCTGCTGCTCCACGGGCATCCCATTCCAATCCAGTCTGCAATTATTGTCCTTGCTGCGTAGGCGGGTTCATTGTCCGCCGCCGACCTGCCACGCCCCCAATTCCGCGTATAAGGCTGTGTC

 

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