CR00531

CRIMIC CR00531

Gene Vha100-1

CG1709

Information

Donor Location Cytology FBti Stock number
pM37-p03R:29131914..2913193298F12FBti0200579Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK CACGGTGCAATTATGGTCCGATCCAT GATAGCGCCGATATTTGCTGGATGAG

Images

Construct Design Sequences

sgRNA sequence GCGTTAAAGGGGACGTTTCATGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
ACTTTCTTTTTGTCCGCGGC TCGCTAATGAGGTAAGGGGT ACGTCCCCTTTAACGCGAAT GATATACGTGCCGAGTGGGG
 
Amplicon 5' Amplicon 3'
ACTTTCTTTTTGTCCGCGGCGGAGAAAAATCAATATACGCATGGGGTATAGAAATCGAATCTCGGCGCATATATAGTATAGATTACAGACAAATAATCTGGGGGACAAGCGAACGTCCGCCCACCGAAACGATAACCAAATCAGCATGAAAATATGTATGCGACACGGCCGCAAAACTTAGGAAGAAACCCAGTTTGGCCATGAATCATGCAATTGAATGGGCCCATTGTGTACTATATATGTACATATGCATTCTGATTTAGTGTCTGTTCTCCATTCGCAGATATCGGGAATCCGGCTGCCACAATGGGTTCCCTATTCCGCAGCGAGGAAATGGCGCTGTGCCAGCTGTTCCTGCAGAGCGAGGCGGCCTACGCCTGCGTCTCCGAGTTGGGAGAACTGGGATTGGTCCAGTTCCGAGATGTGAGTAGGATGGGTTACATAAAGCTGCGGTCGAGGCAGCCGCACTGACTAACCATAAACCACAATCATACCCCTTACCTCATTAGCGA ACGTCCCCTTTAACGCGAATGCAAAGTAAATGCAAAGCTGGTGACACCAGCTACTAAGTGTATAGTATAACTACGTGTGTTACCTCATTTAAATAAAATTGAAAAATATAGTGGCATAAGTGCTATATCTTGATCGCACAAATGAAGTATCTTTGAGATCCCCCAATAACAAACCAATCTTTTGAAATCCGCAGCTCAATCCCGATGTCAACGCTTTCCAGAGGAAGTTCGTCAACGAGGTGCGCCGCTGCGATGAGATGGAGCGCAAGCTGCGTTACCTGGAGAAGGAGATCAAGAAGGACGGCATCCCCATGTTGGACACCGGGGAGAGTCCCGAGGCCCCGCAGCCCCGCGAGATGATCGACCTGGAGGTATACGCCGCCATACTCCCTGTTTTCTGCTAAACACTGTTGAACTTATATGGCTTTCTTGTCTGCTGCTCAGGCCACCTTTGAGAAGCTGGAGAACGAGCTGAGGGAGGTGAATCAGAACGCCGAGGCCCTGAAGCGCAACTTTCTGGAGCTGACCGAGCTGAAGCATATTTTGCGCAAAACCCAAGTGTTCTTCGATGAGGTAAGCTGCGGCATGAGTAACTCCATATGGGTATCAGCTAACAAACTGCGATCGGTTACCGATTTACCATTAACAAATGCCGGCCATTGAAGCGGCCGGTAACTAACCAAATTTTCTAAGCAATAACCAATTTCCCAACCGACAACACAAGTGTGGCAGCCACGACTTCTAGTCACATAAGCAATCTATCCCAAATTACACTCTAAAATTATTTCCCCGTATCTCCCCACTCGGCACGTATATC
 
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