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CRIMIC CR00537

Gene Lnk

CG17367

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:25890495..25890495	96F6	FBti0199404	78942

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCTACGCCGCAAGTTTGTTTTCGTT	ATGCCGTCGATGAACCCTGACCATTA

Images

Construct Design Sequences

sgRNA sequence	CCACTTCAGTGAGGTCTAATCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGCACAGGAGCGAATACGAG	TCGGACTTATGGACAGAATGCA	AGTCCGATTAGACCTCACTGA	CCAGGGAAACACGCTCATCT

 

Amplicon 5'

Amplicon 3'

AGCACAGGAGCGAATACGAGCGCCTTCAGCGCTGGCGGTTACATTGGGCCCACGTCGGCCAGCAGTCATCACAGCCTGGGAACTTCATCGGCGGCAGCGGCAGCAGCAGCAGGAAGTGACCTGATACCCGCACCAATTGGCACGGGCAACGCCATGGGAGTGTCTTCGTATGCATACGGTGGAACCAGTTGGGAGGAGTTCTGCGAACGACACGCCAGAGTGGCTGCCTCGGATTTCGCCAAGGCGTGCATCACGTACATTAATGGCAATCTGCCGCCGGAGGAGGCGAGGAACATCCAGCATCGCAGCTTTGCTCAGAAATTTGTGGAATCCTTTTCGGCGCACTACGACACGGAGTTCTTCAAGCGGAGGAGCACTCTTAAATCAGGTGCGGGCTCGCTGGACTTCGAGGAGGAGCACGAGGTGCCAAAACTGCTCTCAAAGTCTCTATTAAGACGACTATCATTCAAAGGACTGCGCAAGGGCAAGGTGAGTTGATGCTCTTCTCAAATCCTCTCGAAATCATTGAATAGATTGATTTTTGATTATTTCGAGGCCACACTTGATGCTTATACTTTTTTTTTAGACATGATTGTTGTTTTCTGTCCGATAATACCCATTAAGAAGGTCACGAATCACGAACTAAAAGTATGCAGATAAAAGCATTCACTTTAGCATTCTTTGCATTCTGTCCATAAGTCCGA

AGTCCGATTAGACCTCACTGAAGTGGTATTATTATCTTCTATTTGATTTGTTGAATACGTCATGATTATCAGAACATGCTGTCAATTATGCAAGTCTTTCTTTCAATTTTGCAGATCATGAAGTATTTCACATTGATTAAAAAGCTTTTCAGTGCCAGGATGTTTTATAGTCGGAAACTCCGGCTATATTGTCACCACTTGTTTTAACTGTCAAAGATCTCTTTGCTGCAGGCCTTCTTCCACAAGAACTCGGATGACTTGGATGGCAGCGGTGGCAGCGGCAAGCAGAGCAAGACGAAGCTGGCCAAGATCGTTGTGGAGTGCCGGAAGGAGGGCACAGTGAACAACCTGACGCCGGAAAGTCTGGATCAACCGACGGGCTCCCAAAAGTGGGAGAAGTGTCGACTTGTACTGGTCAAGGCCGTGGGTGGCTACATGCTAGAGTTTTACACGCCGCACAAGGCGACTAAGCCGCGCAGTGGAGTCTTTTGTTTCCTCATCTCGGAGGCTCGCGAAACAACGGCACTTGAGATGCCGGACAGGCTGAACACATTCGTCCTCAAGGCAGACAACAACATGGAGTATGTGATTGAGGCGGAGAGCGCGGAGGAGATGCGGAGTTGGCTAGCCACAATACGGTACTGCATGCGAACGCCACCCACTCAGCAGCCAACGATCGAGTCGGATGGTGTTATGGCGTCCGCCATGCAAACATCGCCGACACTTCCGAGTCCCAATCCCATTGGTGGGATTCAGAATCCGCAGTACCAGCAGCAGCGCGGCTCGAATGGCAATCTGGTGGGAGGTGGAGCTCCGCTCACCTCATCGCTGTCTGCAGACAGTGCTTTGGGCCAGGGAGGAGCCACTTCTGCCAGCGAACTAAATGTCATCAACGAATTGGGCACCTCACCGACCTCCGGGCCACCTGACATACCTGTAAGACCCCATCGAGGTGAACAGCGCCTGTCCGCCTCAAGCAACTTCGATGGCATCGAGGGCACGGAAAATGATGCAGATGTGGCGGATCTGACGGCTGAGATGAGCGTGTTTCCCTGG

 

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