CR00538

CRIMIC CR00538

Gene Ser7

CG2045

Information

Donor Location Cytology FBti Stock number
pM37-p0X:9865673..98656779A2FBti020058280590

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK AACCAGAATAAGAGATTCCCAGGCAGC CTTGTAAAACGCCTCCTGGCACTGAT

Images

Construct Design Sequences

sgRNA sequence GAGCGTGGAAAACTGGTTGATGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CGTAGCTAATCAACGCAGCG GTGGGAAACGTGTTTGCTGA CAACCAGTTTTCCACGCTCC CCGCTCACTGTCTCGTACTC
 
Amplicon 5' Amplicon 3'
CGTAGCTAATCAACGCAGCGTCGGCGATGGAAAAGGCAAATCAAAAGCCAATCTCTGAGATTCAAAAAGCTTCCGAGGGTCGCGGCTTGAGGTTGCCGATCGCCAGTAGATCAGTAGTTGATTGCCAGCCACCGGGGACGCAGCTTTGCTAGCGACTCGAGAATCAATCGGAGCGGAAAATAGGAAAATAGAAAATTGATAGGAATTAGAAAATAGGAAAACCTCGCAAAATGCTCCTGCTGATACCGCTTTTCCTGACCCTTTTGGGGGCTGAGGCCCAGCAATTTGGCAGTGAGTTTTACTCAAGACCACCGCTCCTTCGAGATTTCCTAACCCATTTTCAATCCTTTAGAAGCTATCATGCATTTTGGCAACTGCAACTCGGTGGAATTCGGTAGGGGCACCTGTATCGAGAAGAAGGACTGCGACTTTTATGCCGTAGATAAACTTATGGAATTGGCCAGCAAACAACAGTGCTTCTCCCGCCAGCGACCCGATCTGGTAAGTGGTATTATATACTATATATATGTATATATTCGCCCGATGTTCAGCAAACACGTTTCCCAC CAACCAGTTTTCCACGCTCCGTGGAGATCTTCTTCACCTGAATAATGCTATTTATTAGCATTAACAGCTATTTTAACAGTTTATTTACGTCTTACTTTGCGATTTGTACATAATTGATTGTAATACATTTTTTGATTTAAACGGAAATTAGAGAGCTATTAAAAAAACTAAAACGGCTGATATACCCATAGTAAAACAACCCAATCTTCACTTGAGCTTATAGTTTTTATTTTCCAAAAATGTTTATATATACAATGCACCATAATATATATATATCTTATTTATTTCCCATTAGGTTTGCTGCCCCCGCGAGACGAATATTATACCGCCATTGGCACCCAGGATCAGTAATGGGACCACTAATGCCACGAGCAGCACCACAACGCTGAAACTGCTGCCTCGCACCACGCCACGCCCACCCAGCGGTATCGACCAACTGCCCGAGCATCCGTACTGCGGCTCCGCCTTTTCGTTTCGCTTGGTCGGTGGTCATAATACCGGACTCTTCGAGTTCCCGTGGACCACTCTGCTGGAGTACGAGACAGTGAGCGG
 

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