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CRIMIC CR00540

Gene CG9170

CG9170

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:15982226..15982226	14A5	FBti0200583	80591

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	CCCTTGAAACGCTAACGAAAAGGTAAGGG	CCGGTATAGTCTTTGGTATAGTCTTATTCGCG

Images

Construct Design Sequences

sgRNA sequence	TTACATGTGTATAACGATTGAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AATACCCTTGACACCGGACG	GAGGTAGGCGTGTTGACCAT	CGCCTACCTCAATCGTTATACAC	TTGAGGCTCTGTTCGTCTGC

 

Amplicon 5'

Amplicon 3'

AATACCCTTGACACCGGACGGCGAGGTATATCCATCGCTTTCGAACTGTCTATGTGCCTTTTGAAACATCGATACTGTACCCGAAGGTTTCGTACCTCCTTCGTTCTCCTCTGACCTATCGGTTTTTCCATTCATTTGACTAGTGGGCGCGGGTTTATTCGCTATTTGTGGCTTTGACTTGGTCACCTCGAAGCCAAGCTGATGCAATCGACCCATATCCTTGGATCCAGATTCCAAAAATCGACTCTTGGCACTACTAAATAAGCTGGCCTTGCTGGCCACCGACCATTTCCGGGCCAAACTGTTCAGGGGCAGTGGACCCGGCGATGGAATATCATCGATACCCTGCATGCTGCGTATTCCGGAGTCCAGTTGCGACAGATCATCGTTATCCCCCACCGAACCCGTGTCCAAAGGCTTCTGGCGCGCCTGATTAACCAAATCGCGATATATGGCATCTAGTGGATGGTCCCATTGGGACTCTTTGGTGCGCGTATTATGGTAGTAATGGCCATTCTTCTCCTCCGAGTAGCTGCCATGAAGTCATCCAAAATATAATTACTTTTGCATGCATTTTCAGTCATCTTGCAATCCTTACCATATCTTCCAGTCTGTTGGCAATGCGGCCATCAATCCTTCCTTGGCCAGATAGAGCAAATGCGGTTCCTTCTGAGGATCGATTCCAATCAGGGAGGCATACTCATTGATTTCTGTGCAGGAAAAAATGGTATATACATATTTAAATAAAAATTTGTTGCACAACTTTTAACAATTAATAATGAAATATGCTCTCAATATATTGTTTTAGCATAGTGAAGGAGATGAAAATGTGTTTGGTAATTTTTACAGGCTATAAAAAAAAGACGATAAAAACTAGTATATAACCCTATTTTATATACATATATATTTTTTTGTAAAATTTTAAATTTCTATTCTTTTGATTTTCTTCTTTATTTTCAACTTGAATATATAATATATAATAAAATTGATTTTTGCAGGATAAAAAATGTCTTTCATTGATGTATATATATGTATATACCTAAGAACATATGTATATGCTCCAAATGGTCAACACGCCTACCTC

CGCCTACCTCAATCGTTATACACATGTAAGAAATCACTTTTTATTGGATTCGAGGCAAACGAATTGCTTGCCAAACGTGAATTTTCACCGGAAAAGCCGATATTACATACTCTCACCCACAATTGGGGTAACTAGTCAACGGAACTTACCCTCCGATGAGGGGAAACACGCCTCGTCGAAAACCTCCTCGCAGATGACGGACGACGAGCCCTGGGATGATGATGATGGTGTGGCTGGGAGGGATTTGGTTAACTCAGCGGACGCCGTGCTCGAGGAGATCCTCTTGCACACGCTGGTCGTCGTCATAATGGGTACTTTAACTGGATTTCACTAACCGAAGAAACTCGAGCGGAGCTAGCGTTTTCGTTGCTCCGGGAGTTATCCGTACTCGAAGTGTCGTTCAATATATTTGCATATATTGTTATACTTTTCGGATTTGACATTCATAGTTCTCCATACTATTCCTTAGCGAGTCCTGCCGACAGCGGAGCAAATGCCAGAATAATCGGATGCAGTTTGACCTAAAAATGAAAATACAAATACCTACTGAGTCATCGCTATACCACTCAGTATGTGCGTGTGTCCATGTCGGAGCATGCTCCGAACGTGGGTTGCTGGCCAATTATCTGGTTGGCAACAGGATATTGCAGCGAGTTTGCGTGTCCGCGCAGCCCGACGACTCCCAGGTTCCAGGTCCCTGTCCTTTCCACCACGAAAGCAGACGAACAGAGCCTCAA

 

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