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CRIMIC CR00545

Gene Cyp4e1

CG2062

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:8447709..8447721	44D1	FBti0199406	78944

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCCAAGGAACTGTACGAAGAGGTCTC	CATTTTCTTGCGGGAGAACTCGTCAG

Images

Construct Design Sequences

sgRNA sequence	ATCGAGCGATGTTATCTAGAGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CCACACTGAACCTGGGTCTC	GGGGACTTTGATCCCACACA	TCGCTCGATAGGTGTGACTG	TGACCTCGTGAAAGTCCTGC

 

Amplicon 5'

Amplicon 3'

CCACACTGAACCTGGGTCTCGTGATGCTGGGTTATAACGAAAAGGTGTTCAAAGATCCCCACAAGTTCCGACCTGAGCGCTTTGAGCTGGAGAAACCAGGACCTTTTGAGTATGTACCCTTCAGCGCTGGACCCCGAAACTGCATTGGTCAGAAGTTCGCCCTGCTGGAGATTAAGACTGTGGTATCCAAGATCATTAGGAACTTCGAGGTGCTTCCTGCTCTGGATGAGCTCGTCTCTAAGGATGGCTATATAAGCACCACTATTGGACTCCCTGATGCCGAGAGGAAGAAGCGCGATCCATACCGTCACAAATACGACCCTATTCTTTCCGCTGTGCTGACCCTGAAATCCGAAAATGGTCTATACATTCGGCTGAAGGAAAGGCACTAAAATGTCAATTTATTGATCTCTGTTAAGTTTGGTGCTTTAATTTTGCATACCTGTGTTTTTTTAGGTATGCCGTCCCCACAGATACAATTATTAGGAAGCTGTTAATCACTTTGTCTCATACAACACATAAAACTTTTGAAACAAATCAACTCGTTTTAATCTTTGAACCATTGAGACTATGTTAATTAGCTTAAAAAATTTGAAATTATAGTTTCCACACGTGTAAGCCATTTTCAAGAGAAATGTTCATCAAGAAACTTAGTCGCAGCATATAGAAGGTCATGCTGAGTGCCCATATATTAGTTTAAAATTTGGGAGATAGTCATAGGCTGCTTCTTATGTGTGGATGTATATCCAAGCGCGAGAGACTGAGAACTGTCTGCCGCAACCGGTTGAAGTACTGACGCAGAGAGTCTTGAGGTTTCTACTCAAATCCGATTTACTCGCTGCACCGATCGGTTGTCTGTGGTCGCATTCAAAATGTGGATCGTACTGTGCGCTTTTCTTGCCCTTCCACTGTTCTTGGTGACCTATTTCGAGCTTGGACTACTGCGGCGAAAGCGAATGCTGAACAAATTCCAAGGCCCAAGTATGCTGCCTTTGGTGGGCAATGCTCATCAGATGGGAAACACTCCCACAGGTAGGTGCTCCATCTAGACAATCTCAATGTGTGGGATCAAAGTCCCC

TCGCTCGATAGGTGTGACTGATCACATAACAGTTGTGTACGAATGATAACACTGAGACTAAATATACTAAAGGTCACTGCAGTACCGATCAATGGGATTAACTTAACAAATCTTCGTCAGGGAATTCATCCGTCGTTTTTTGCTGTATTAAAGACTATGGCTTCTATCTAATTGTAGAGCAAGTAATGATATGTTTTAACTTTGCTCTCCAGAGATTTTGAACCGGTTTTTTGGCTGGTGGCATGAGTACGGCAAGGATAACTTTCGTTATTGGATAGGTTACTACTCCAATATTATGGTCACTAATCCCAAATACATGGAGGTGAGTGTGACATGTTAATAACGCTAAGGCGAGGATGGTAACAATGCAGCTTTTGCGCAGTTTATACTTAGCAGTCAAACTCTGATATCCAAGTCCGATGTATATGATCTTACTCACCCATGGCTGGGATTGGGCCTCCTCACCAGTACTGGCAGCAAATGGCACAAACACCGGAAGATGATAACCCCAGCCTTCCACTTCAACATCCTGCAGGACTTTCACGAGGTCA

 

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