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CRIMIC CR00546

Gene Ent1

CG11907

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:357318..357318	21C1	FBti0199407	78945

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TGGGAGATGAGTTGCTTAAGCCGACT	CCATTATCGACGAAGCCAGCTCCTTT

Images

Construct Design Sequences

sgRNA sequence	CTGGGCCAAGCGCAATTCTAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCGACATGACCTGACGCAAA	AAGGCGCGGTGCTTATCAG	CCTTAGAATTGCGCTTGGCC	CAGTGAGGGGGTCAGGAAAC

 

Amplicon 5'

Amplicon 3'

TCGACATGACCTGACGCAAAATAGGCTCAAGCGGCAAGCCCTCAGCGCTTCCATTCCGGTTGTGCGAGGGCACTGCCCGGATGACCTTGTACTTGTCGCCACCTTCGAGATAATACCTAAAGAAAGGCTTTCGAGCCAGGATTACGTAGCACACAATGCAAAGCAGGATGAGCACTCCTCCCACAATGAAGAAGATGAATGCAGTGGTATTGGGACCCGTGTCGAATGCAAGCACAAGAATGAAGGCCAGGGCTGTGAGGATACCACCAAGAGCCTGTCCGCTAACCACAGCGGTGATAAACTCGGAGGGAAATAGACCAGCGACTCCGTAGAGGGCTCCCGACATTGTAGCCGCAGATACTATATATAATAAATATTAAGTAAACACTCAAGGAGTACTTCATGGTACCCACTATTTAGCAGCACTACAATGATGAGAGTGATTAGGAAAAACTGTTCCTGCCACGTGTCTGTGTTGATCTCCACGAAGCCAGTTGTAACCCCGAAAAGGATGAGGATCATCCACAGCGTTCCTAACATCTTGGTGCGCAGGGACACATGGTGTCCGAAAATGGCGTTTAGCAGCAGAAATGTAGTCCCAGAAATAGTGGCCGTGAGAGCCAAGTCGCAGGTAAAACTCTTCTGCAACGGGGTGAGCTCTTCCTCCAGGTCTGTGTTATTAATCGATGCATTCCGGAACTTGTACTTCCAGTACTGAAAGAGAAGCGAACAGGGTTAAAGCGTAAGTCAGCGGAAAACAAAGAAAATCAAAAAAGAGAGTTTTCGTCTGGGTGTGGGTCGTAAAGAAACGACGACAAAGCTGATAAGCACCGCGCCTT

CCTTAGAATTGCGCTTGGCCCAGTTGTTCAGCTGTTTGGAATTGATATGTCCGAAGTTCAGTTAAATTGACTAGGATGCGGGTGCGGAAATTTCCACTCGTTAAACTTACATCTTCCGCAGTCACAAAGAAATTCCACGGGGTCATGGTACCAATGCCCAACAGGTAGAACACGAGGTAGGTGAAGAGTCGTCCACTCTCGGGTTCGTTGGACACCAAAACTACCTCGTCCTGCTGGCGTTCCATGAGACATTGCTCATCCGCATGGTGGTCCTGCGGACGAAAAGCGGGAACGAAGCTGCCCTCCGTGCCCAGAATCTCGTCATCCTCGTCCAGGGCGTCCACCAGTCGTCGTGTGTCACTGGTGCCTGGCATTGCCACTGAATTGCACTACTGTTTTGTACGCAGCCCTTGGTATTCATAAATTTACAACACCTCTGGCGGATTATTCACATCTGTAATTGTGGAGAAGTATTCGATGTAACTTAATCTGATGCAATTTACTTTCATGTGTATCATTTAGCACGGACGAACTGCGTAACATAAAAACGAATTTGTAAACAACAAAGCAAGACATTATACAAAAAATATCGATGGCTTATCGAGAGGAATCGATTAAATGAAAATATCTAAGATATCGCACTAACACCACAAAACCGTTCACCCAGGTTGGCAGCCCTTGGCGCGAAATGTGATGGGATTCAAACTGCATCTTAGTTTTGCATCTTAGTCCTATATTTTAGAGGACTGTGAAATTGGATTTTATTTGCAAAACGAGCGTAGCATCCACCTCAAATCGTAATTTAATTGCCAGCCAGAGCAGCAACAATAAAAACACCAATATCAAGCAGACCCTAGAGCTGATGATGAATGAACGCAATTTGTATTTAATCAATAAGCACGCCCCGCAGTGTTTTAAATCAGCGCACAGCTTCGTTTTACGGCTTAGCGCCATTCGCAGGATGCGATCCGCCCTCCACCCCTCGCCCATTGATCGCTTGCGAAATTTTAATTGGATTTGCATTCGCGTTCGTCCCGAAACCGGTTAACACACGCTCCTTCCCTCTGGACATGCGTTTCCTGACCCCCTCACTG

 

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