CR00547

CRIMIC CR00547

Gene CG12118

CG12118

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:9195410..9195410	8D1	FBti0199408	78946

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAATCCAGTAGCAGCTGTGCGTCAAA	AGCTGTGCTGTTAGGCGCATATCTTG

Images

Construct Design Sequences

sgRNA sequence	AGTTTAGCACGTTGAAAGCTAGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGCTGGAGCAACTCAACCTC	TAGGTGGGTTGACCTTCCTT	ACCCACCTAGCTTTCAACGT	GGATGCCGGCGGATACTTTA

Amplicon 5'	Amplicon 3'
AGCTGGAGCAACTCAACCTCGATGCAGCTGCCGTCGACGAGCTGAGTCGCGTTTACGAGGACAGCCGCAAGGATCTAATTCTCCGCCAACTGCAAATAGGCCATAGCTTTCCGCACATTACCGACATCCAGTGGCGCATCGTGTGCGACGTGAAGTCTTCCACCTCGGACTGCAGCACGGGCGCGGCCTGCTTCCACATCAATCTGGGCAACTTTCGGCAGAGCAGCGGCGAGCGGGTGACCATCGTTGAGTTTGTCTGCAATGCCGAGGAGCTGCAGTCGCTAATCAATCGTCTCAAGGAGATCGAGCGGCACTGCCAACAGATGGCCGTGGTCTAGGGAAACAAGCTCTTCAAATTCTTATCGATTATATTTCGCAGCATATAACACCAGCATTGTCGGCTATATACAGTGTCGGAACAGGAAACCTAAACAGTTAACACAGTCATCATCGCTTAAAAGGCTACGGCTTCATCATTTGGAGTCCAACTCCAATGGCAACCACTTCGTTGATCATCCGCGTCAATGGGCAACCTTGTGCGGCGGCGGCACAAGCAGCTTCATCAGCTGCCCATAGTGATTCGGCGCTGTGCTATAGATGGTGCCCGAGAAGCGGGTGCTGTCGTTCTTCTCGGCGGAAATCACTGTGCAACGGTTCTGTTCCGAAAGCCGCATGTTTAGACCACGGAAGCGTGAGTCCAGCCTGTATAGATTAGCCCCTTCCCGTGGTAGAAAGAATGGACGTCCGCTAAACGGATTCATGAAGTCCGCCCAGTAGCCGTACGATAGCAGGCGATCGCACATCTCCCGCGCTGCCAGGACAAACTGCAAAGGTAGTTGGCATTAGTTATGTATGTAGATATACTCGTTTAGATAATAGGTAAATACCAACAAACTAACTTCTCTGTATTCTCATTCATCAACCCAAATCTAAAACTTATTACAAATGATTGAAAATGTTATGGCTTACTTTAAAATTGGGTGCCAAACTTAAGGAAGGTCAACCCACCTA	ACCCACCTAGCTTTCAACGTGCTAAACTGGTTTGATTGTATATAAACAAATGATCAGAGCAATAACGAGGTCATTCCTCCAATTCATCCAAATACACTGCAGTGTGATTTCCCGCTACAGTGTGGATGCGTGAATAAAACTGATTTCTCACCTTGGCAGCGCCCATTTCGATGTCTCCTTCGTGGCTGAGAACAAGCATGGTGATGTTTATGCACTGCTGTGCGACGACGGGTACCGGGAAGATCTCCAAGAGTGGCACACGGATAAGCAGGGGACACTTACAGCAGGTGAATTCCAGGCGCGATTTGTCCGTATTGGGACCCTTGTGAAAGTTAACCAGGTGGCCAAGCGGCTCGAGCAGACCGACGGTGGTGCTGTCGCCCTGCCAGGCGGGCCCAATGCCATTTGGCAGATAGAAGCGATTGCCTCTTTCGGCGGTCAGTTCCCAGTTGGGCTCACTCTCCAAACTATTTAGCTCATCGTCGTCGTCGAGATCCCGCGACTTGACGGTCTTAAAGTATCCGCCGGCATCC

Amplicon 5'

Amplicon 3'

AGCTGGAGCAACTCAACCTCGATGCAGCTGCCGTCGACGAGCTGAGTCGCGTTTACGAGGACAGCCGCAAGGATCTAATTCTCCGCCAACTGCAAATAGGCCATAGCTTTCCGCACATTACCGACATCCAGTGGCGCATCGTGTGCGACGTGAAGTCTTCCACCTCGGACTGCAGCACGGGCGCGGCCTGCTTCCACATCAATCTGGGCAACTTTCGGCAGAGCAGCGGCGAGCGGGTGACCATCGTTGAGTTTGTCTGCAATGCCGAGGAGCTGCAGTCGCTAATCAATCGTCTCAAGGAGATCGAGCGGCACTGCCAACAGATGGCCGTGGTCTAGGGAAACAAGCTCTTCAAATTCTTATCGATTATATTTCGCAGCATATAACACCAGCATTGTCGGCTATATACAGTGTCGGAACAGGAAACCTAAACAGTTAACACAGTCATCATCGCTTAAAAGGCTACGGCTTCATCATTTGGAGTCCAACTCCAATGGCAACCACTTCGTTGATCATCCGCGTCAATGGGCAACCTTGTGCGGCGGCGGCACAAGCAGCTTCATCAGCTGCCCATAGTGATTCGGCGCTGTGCTATAGATGGTGCCCGAGAAGCGGGTGCTGTCGTTCTTCTCGGCGGAAATCACTGTGCAACGGTTCTGTTCCGAAAGCCGCATGTTTAGACCACGGAAGCGTGAGTCCAGCCTGTATAGATTAGCCCCTTCCCGTGGTAGAAAGAATGGACGTCCGCTAAACGGATTCATGAAGTCCGCCCAGTAGCCGTACGATAGCAGGCGATCGCACATCTCCCGCGCTGCCAGGACAAACTGCAAAGGTAGTTGGCATTAGTTATGTATGTAGATATACTCGTTTAGATAATAGGTAAATACCAACAAACTAACTTCTCTGTATTCTCATTCATCAACCCAAATCTAAAACTTATTACAAATGATTGAAAATGTTATGGCTTACTTTAAAATTGGGTGCCAAACTTAAGGAAGGTCAACCCACCTA

ACCCACCTAGCTTTCAACGTGCTAAACTGGTTTGATTGTATATAAACAAATGATCAGAGCAATAACGAGGTCATTCCTCCAATTCATCCAAATACACTGCAGTGTGATTTCCCGCTACAGTGTGGATGCGTGAATAAAACTGATTTCTCACCTTGGCAGCGCCCATTTCGATGTCTCCTTCGTGGCTGAGAACAAGCATGGTGATGTTTATGCACTGCTGTGCGACGACGGGTACCGGGAAGATCTCCAAGAGTGGCACACGGATAAGCAGGGGACACTTACAGCAGGTGAATTCCAGGCGCGATTTGTCCGTATTGGGACCCTTGTGAAAGTTAACCAGGTGGCCAAGCGGCTCGAGCAGACCGACGGTGGTGCTGTCGCCCTGCCAGGCGGGCCCAATGCCATTTGGCAGATAGAAGCGATTGCCTCTTTCGGCGGTCAGTTCCCAGTTGGGCTCACTCTCCAAACTATTTAGCTCATCGTCGTCGTCGAGATCCCGCGACTTGACGGTCTTAAAGTATCCGCCGGCATCC

Search Again