CR00548

CRIMIC CR00548

Gene CarT

CG9317

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:20729802..20729808	38E4	FBti0199409	78947

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGGCAAGGAACAGTGGAGTAACGAAG	GCGGATAGCAAGGCTTTGGATACTAG

Images

Construct Design Sequences

sgRNA sequence	CATTGATTGTGCGTTGCTTAAGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGATTCCGGCATGACGAACA	TGAAGGGTCGATACGAGGGT	AGCAACGCACAATCAATGGG	TTACGAATCGAGCCCACTGC

Amplicon 5'	Amplicon 3'
TGATTCCGGCATGACGAACATGTACAGGAAATAGGCGTAAAACGGCAGGGATGTGATGTAGGAGAGGCGCACCCAATCCCGCTCCAGATAAGTGACGCCGGACAGGAGCATGATGCCCGAAGTGTAAAAGGTGCACGTCATCACAGTCACGAAGGATCGATAGTTTTCACCCACCAGTTCCAGAGCTGCAGACGCAGAAAAGTTAATGGTTAAAGTTAGAAGCAACAATGAAATACCCACATATTATAAATGGTATCTGGTAGACAGCTGGAATGGTCAGTCCTACGATGACTCGACTGCCGGCCCAAGTCCAAAAATCCTTGCTCAGCGAAGTCATTAGGCTGCCAGCCAGCAAAGTGGCCAAACAGACGAAATAGGAGAGCCTTCGTCCACCACGATCATTGAGTAATCCGAAAAGATAGACTCCCACTGGACCGCCAGTATTAAGAGCAGCCAATCCAATCGTAGGATAAATATCCTGATCGCACACTAGATCGAACTGAAATAGGAGGATTTAATGGTTTATAGTAAGCCAATCCTTCGCTCCTAGGTAAAGGTAGTACTGATGCTTGGGAAATGATTCGAGCAACTCCTACATGAATGACGTTGTAGTTTCAAATGAAGGCTTGGTGGTCTGGATTGCGCACATCAATCACAATGGAGGACCAGACGACGCTTGTGTTGTACTCCCATCCCTGGGGGCACTTGATCAGTGGCCAACTGGCATTCGGCTCCATGGTCGTCAGGTCATCCTCTTCCCCCGATTCAAGGAGCTGCGTCCAGTTGACTCCGTAGGTATAACATTTGCTGTAGACCAGTTCGTCGTTATCCTTGAAATAATTATTTCATCAGTTATTTCAAAATATATTTAAATAATAGAAGGCATAGAATATAAGGTTTACCTCTCCCCCATTTCATTGTTAGCCCCTTCGATTACTCATCGAACCACCCTCGTATCGACCCTTCA	AGCAACGCACAATCAATGGGCTGACTTGATTGCCTTAGCCTCGGCACTCGTATGGCTGCAATTACTCACCAGTTCCTTGGGTATGGACAGCGATTTTCGCTGCTCCAGGGGCAAGTCGAGTAGCTCCGGGATGCGACACCAATAGTCATCCGGAGTATCAGCCATAAACAACTGGTTAAACGCACAAAAACCGCACGGTATGCAGGCGGGCAGGCAAATTCCGAAAACCAAGAGCTTCTGGTATTTGCCGAATTCGCCAATGGTGGGCAGAAGGTCGTCCAGATCGAAGGCCTCCTGGAAAGAAAACAGCAAATCTAGAGAAGATCAAGGACCGTGTCTCACAGATATGCACATCCACCGAGGGCTGGGATTCGGGCTTGTGGCGTTGTCGCAGCTCCGAAAGTTCATCGTATTCGTCCCCATCGTTGTCCTCAATATCTGACATGGATGCGCCTCTAGACAAGCTCTGAGCTGGAAATGTTTACTCACACTGCCCGATTGGCTCAGCTCACGGAAGCCATAGAAGCAACCACTGACAACTGTCAGTTGTCTAGGGTTGAAAGAATGAGCTGGTTCTGTTGATAGTAAAAAGTTTGTCGCAGTGGGCTCGATTCGTAA

Amplicon 5'

Amplicon 3'

TGATTCCGGCATGACGAACATGTACAGGAAATAGGCGTAAAACGGCAGGGATGTGATGTAGGAGAGGCGCACCCAATCCCGCTCCAGATAAGTGACGCCGGACAGGAGCATGATGCCCGAAGTGTAAAAGGTGCACGTCATCACAGTCACGAAGGATCGATAGTTTTCACCCACCAGTTCCAGAGCTGCAGACGCAGAAAAGTTAATGGTTAAAGTTAGAAGCAACAATGAAATACCCACATATTATAAATGGTATCTGGTAGACAGCTGGAATGGTCAGTCCTACGATGACTCGACTGCCGGCCCAAGTCCAAAAATCCTTGCTCAGCGAAGTCATTAGGCTGCCAGCCAGCAAAGTGGCCAAACAGACGAAATAGGAGAGCCTTCGTCCACCACGATCATTGAGTAATCCGAAAAGATAGACTCCCACTGGACCGCCAGTATTAAGAGCAGCCAATCCAATCGTAGGATAAATATCCTGATCGCACACTAGATCGAACTGAAATAGGAGGATTTAATGGTTTATAGTAAGCCAATCCTTCGCTCCTAGGTAAAGGTAGTACTGATGCTTGGGAAATGATTCGAGCAACTCCTACATGAATGACGTTGTAGTTTCAAATGAAGGCTTGGTGGTCTGGATTGCGCACATCAATCACAATGGAGGACCAGACGACGCTTGTGTTGTACTCCCATCCCTGGGGGCACTTGATCAGTGGCCAACTGGCATTCGGCTCCATGGTCGTCAGGTCATCCTCTTCCCCCGATTCAAGGAGCTGCGTCCAGTTGACTCCGTAGGTATAACATTTGCTGTAGACCAGTTCGTCGTTATCCTTGAAATAATTATTTCATCAGTTATTTCAAAATATATTTAAATAATAGAAGGCATAGAATATAAGGTTTACCTCTCCCCCATTTCATTGTTAGCCCCTTCGATTACTCATCGAACCACCCTCGTATCGACCCTTCA

AGCAACGCACAATCAATGGGCTGACTTGATTGCCTTAGCCTCGGCACTCGTATGGCTGCAATTACTCACCAGTTCCTTGGGTATGGACAGCGATTTTCGCTGCTCCAGGGGCAAGTCGAGTAGCTCCGGGATGCGACACCAATAGTCATCCGGAGTATCAGCCATAAACAACTGGTTAAACGCACAAAAACCGCACGGTATGCAGGCGGGCAGGCAAATTCCGAAAACCAAGAGCTTCTGGTATTTGCCGAATTCGCCAATGGTGGGCAGAAGGTCGTCCAGATCGAAGGCCTCCTGGAAAGAAAACAGCAAATCTAGAGAAGATCAAGGACCGTGTCTCACAGATATGCACATCCACCGAGGGCTGGGATTCGGGCTTGTGGCGTTGTCGCAGCTCCGAAAGTTCATCGTATTCGTCCCCATCGTTGTCCTCAATATCTGACATGGATGCGCCTCTAGACAAGCTCTGAGCTGGAAATGTTTACTCACACTGCCCGATTGGCTCAGCTCACGGAAGCCATAGAAGCAACCACTGACAACTGTCAGTTGTCTAGGGTTGAAAGAATGAGCTGGTTCTGTTGATAGTAAAAAGTTTGTCGCAGTGGGCTCGATTCGTAA

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