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CRIMIC CR00552

Gene Vrp1

CG13503

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:22109492..22109499	58B8	FBti0199410	78948

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TTGACGGGACTTTGTGGTTATCCTCG	TGTGTGTGCTGTGGATTGTAAGCCTG

Images

Construct Design Sequences

sgRNA sequence	ACGCCCAAGGTAACCACCCAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTCCCGAGCCTGATGACAAG	GGTTACCTTGGGCGTTTTGC	GGCATCGCAGTCCATTAAAGT	CCCTAGTAATCCAGCACCGC

 

Amplicon 5'

Amplicon 3'

CTCCCGAGCCTGATGACAAGCCCGAGCTGCTGCCAAACTGATGCGAGGCATTGGCCATGTGCGTTGGTGGCGTCGATGGGGCGTTGGGCCGAATGGATGAGTGACGCTGCGGCGGAACTGGCGCTGATCCCGATCCGGAACCACCCTGTAAGAAACGAGAAGTCACTTAGCTTGATTCTACTGCGGTTTTTGGGGCTATCATAGCGTGTAGTTACGTTGCGCTTGCTGTTAAGCATATTGATAACCTTGACTGACTCGCCACTGTGCCTCCCTTGGCGATTTATATGGAGTATCTTCTTAAAGGAGTACACCGTCTTGTCTATCCAGTACTTCACTCGGTCCTGCTGGAGGAGCACATGAATGCGGGCCACTGGGCCACGGAATACGCTTAGGGCAATGGGTTGTGTGTAAAAGCCCAGTGTTTTGGTCACGTCTCGTTTGGAAAATCAATCCACCCCGCTCAACACAAAAATAGGTGAGTTATAAACGGAACACTAGCTTGGACTGATATGAACAGGCAGTGCGCAGCCAGGAGCGCTTACAAACAGGTGAGACTGCGCAGGCGCAGTGAGAATCTTAATTTAAACGAAGACAGATAGGGTGAAAAGGGTGGCCCGAGTTCAAGCGAGCAACAAATGCACTGCGCAAAACTAGATGATTACTTAAGGATAAGTGCCGAGCAAATATACATCGGCTCACCCATTATCTGGTTTACCTTTAAGAGCATTTCGAATGACTAAGAATAGTATTAATTTACGTGCTCAGTATGGTCAGTCCTAGTTTACTTAAAATATATGTATATCCCTGACTTCGGCCCCACATCTGCTCAATACATAGGCAAAACGCCCAAGGTAACC

GGCATCGCAGTCCATTAAAGTTCACCTCTGACATTCGGGCCAACCTGTTGTTCTGCGCTGATATTATTCCTGTCCGTTTGCTACTTACATTGCTCGACTGTCGCTGTGGAGGCGGCGGAGGTGGGCAGCTGCGGTGTGGTGGCGGCGCTGGCGGATTTAGAGGACCTCCATTTAGGATAATAGGTTTGACATTGTCTCGCAGCAACGATTTGGGACTGGCGCTTGCTGAGGATGGATTGGACGAGGGTGCGGGCGTCGGCGGCAGTGGCGGTGATGGTGCTCCGTTAGACATTCCGGCGCCTCCACGGAATTGGTCGCGCAGGGCAGCCACGCTATTGCTGCTGCTCGACGATGATGGCGGCTGGGTAATTGGCGGTGAGGGAGCCTGAGAACTGATGGCCGAGCTCTGGATAAGAGCTGTGTTCACCGCACTGAACATCGTCGATCCGCCGATATTGTTCAGATTGGTGTTGGAGAAACTGCGAGCCGGCGTGGGTGGCGGTGGTGGTTTTACCGATGGGCGGGCTGTAAATGTATGTTGAACAATTAGAACAAGCGTAAGTTAAATCTCATCAATTTCAATATTATCAGTAAAATATACAATTTTGAATTCGTTAAAAGATTAAGATTGCCGTATGGGTAACAAAAAAGATAAAACCATTCATTTATAGTTTGAACTTTGAAATTACCAAGGAAAGGACTCTTACACTTTGGCGGGTTAGGGGCACATCTCATACTGCCTGCACTGGTGTTGGGGCTGCTGGATTCGCTGCCATGGAACTGCAGTGGACCGCGCAGGGTGCCTAAATGCTGCTGGGTGGGGAACACAAGGCCGTTGACGATACCGGCCGCAGGCGGAGATCTGTAAAAGAAACTTCCCCAGATTAGCTGAATTCATTTTAGGTCCTCCTCGATTGTTAAAGCCAAAAAAGCAAGAAGCGTAAGAAAAACCACAAACGGAGTTTGATGAAAGCAGATGAGCGTTAGCTGCGGTGCTGGATTACTAGGG

 

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