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CRIMIC CR00553

Gene Syt14

CG9778

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3R:4317574..4317574	82A3	FBti0199411	78949

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GAGCCAAGATGATTGTTACCTCCGCT	ATCTCTTGTCCGATGCTGCTGACCAT

Images

Construct Design Sequences

sgRNA sequence	AAAGGCATTCAACTTTCGGTCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTGACCCCGTTGTAGCTCAA	TCGGACTCGTAAAGTTTACCCA	CGAGTCCGACCGAAAGTTGA	GGATACGGGCTGGGAATCAG

 

Amplicon 5'

Amplicon 3'

GTGACCCCGTTGTAGCTCAAACCCAGCAGCAACTCGGAGACGCCCCCGTGCTGCATGGACGAGGTGGAGCCCGCGCTTTCTGACCGAGCCAGGCTTAACAGATCGCTAGTAGAACCCAGGCCCGACGAGGTGTTTCGTGGCTCCAGCGGCAGCCATAGGTTTGTTTCCAGTTCAAGGTCAACAGTGGCGAAGCTCACATACGCCTCCCCAATCAGGCGTTCCTTGCGCAGCCGCTCGCAGCCGTAAAGCCGCACACGCAGCCCCATGTTGTTCACATCTTCTGGGTTGACGCGATGGAGAAGAAAGCTCTCCATATACTGTGGATTTTCGCCGCTACGGATCTTGGTTTTGAGCTTCTGCTTTTTGCTTGGCAGCATCAGCATCCGGACCTGCGTGTGGGTGGTCTGCCCACTACCCAGGGGCGGAAGATTTTTTGCCTGCATCACGTGCACCGTCATTTTCCGCATTGGGGCGTCGTACAGCAGCGACAGCTCCAGCTCGATGGGACCCCTCTGCACGGCCGGCTCCTTCTGGTCCACACCAACCAGTAGGTCATCCGACTTGATCGAGCGCAAATCGCTGGTGTCAAATAGGGGTTCCTGGAAGTTGAAAAGTTTTGTATGTTAAAAAAAGTTTCAATGAAAAGCCCTAAAGCATGGTACAAGAAAATATGAAATGCTATCGTTGAGTGCCTCGACTATCAAGAACGCATTACCCATCAAAAAGATTTTATAATAAGAGCCTAGGGATAAATAGTTTTTGGAATATCAATTGGTACTCGCAAGTAAAGTAACTTAAACACTTTAATTTATTTTTTAGCAAAGTATCGGATTTTGACAGATTAATTATATTTCTTAGAAAGAATTTTTAATTTATGCGGTGCACATAATTTTTCAAAGTTAAGATAATAAACTTTTATTTTACACATGCAACGCATTAATGAATTTATACAACGCATTAATGAATTTTAACTTGTCAAGCAAAAAAAGATTGTTAACAGACTATATGGACCATAAGAGTCGGTTAGACTCGACTATGCTTGTTGGGTAAACTTTACGAGTCCGA

CGAGTCCGACCGAAAGTTGAATGCCTTTCTAGGCATAGTTTCTTTTAAAGGAATAGGTATTTAAAGTACCTCTTATGTTGTAAAACTTACGTCATCGTTGTTGTTGCTGCTGGGATGGTTATTGCTGGTTGTTATCGAGTTGTTGTTGTTGTTAATGCTGTAGGCATTGACCATGGGCACCACCAGCACGTTGTCCCGTTCCTTGTCCAGCTTTGGCGGAGCAGCCACGTTGACCCGGTGCTGGTACCTCAGTGCCTGGGTCTTGGCCCGGTGTTCTCCACACGGATTGCGGAAGGCTGTCGCCGGGTGGGGAACTGTGGTTGTGGCTGTCAAAAGGTCGTGACTCATTGCAACGGCTGTCAATTAGCTGGCCCTGACCCCGCCTTGCTTACCTTTGCTCAGACCCGTCAGGACGCCTGTGTGACTGTCCACGGAGACCTCCATGGAATCGTTCGAGCTGCACTCACTGTGCGATGACGGGATTCCAACCTTGCCACGCTCGGCGATGGCTAAGGGGTCACGCGACACCGTCACACGCTGCAGGTCGGCACTTGCGCCTGCTTGGTGGTAGTTGAAGCTCTGCGCCGTCAGCGGATTAGCCTGGCTGATTCCCAGCCCGTATCC

 

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