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CRIMIC CR00558

Gene parvin

CG32528

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	X:19746418..19746431	18E5	FBti0200585	80592

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	AATTCTGACGGCGCCCTTAGCTAC	CTCGTTCCATCCGGGTTTCACTCA

Images

Construct Design Sequences

sgRNA sequence	AGTGGCCCAAAATGACCCAATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GCCTCGAGTATTCGGAGGTC	GGCCACTTTTCGGAGGTTCA	ATGGATCCTCCCCCTGCG	AATCACGACGGTCACGAACA

 

Amplicon 5'

Amplicon 3'

GCCTCGAGTATTCGGAGGTCTTCTCGCCGATCTTCGTTCTGTTGCAGCTCGTGGCTGTGCGCGCCTGCCAGTTGCCCAACTCCATCTAAACCATAGACGTAGTATAGCCCTAGTTCCGTATTATACATATATGTAACTATTCTGAAAGCCTTTAAATCATTGGATTGGTAGTTATCTGTATACGCAATGTGGTACAATAATTTTTAAGCGGTACGAAATTTATCTTTCTGGATATAGCATATCTAATATATACGAATTATATCTGATCTGATTCGGTATGAAGGACATACATACATACATATGTTGTTGTGCTTTTTAATCAATGATCTAATGATCTACGCATTATAATTTTTTTAAAATTTCCAACAATTTAATTTGATGTACAAATTCAAAAACTATTAAGCTGATATGAATTTCTTCTAGCTGTTGTTGTCAAAAGCCACATTTTTTTTAAATTATAAATGAAAAATAACATTTAAGCTCATTGTTTTAATTTAATCTAAGGTCTTTTTAAACAATTTTTATAATTGGCAATTTAGTTTTTATTCGATTTTTTTGGAAGTTTCTCTTGAGTCCGAACGCCAGTGTTGGACAACATGTGTGGTGGGCCTGCGATCAGGTGGCAGCCCCAAGGGAAACTGATTGCTCAGCTCCGCCCGAAACGAATCGAAACAATAACACAAACTGTTCACTTAGTCCGGAATTCTTGGAATCCAGCAGCACCATGTCCACACTGAACCGCCCTAAATCCCCGCACACGCCCACCGCCATTAAAAAGGGCGAGAAGGAGGACAGCTTCTGGGACAAGTTCAGCACTTTGGGCCGCAAACGCGGCACCCGCGAAGGTAAGTCCACTTGATGATTGGAGCTCTCCAGATGGCTATTGTCGGCCAGGAATTGATGGGTATCCCAGAGTATTCCATTCTTGGACCCCTGAACCTCCGAAAAGTGGCC

ATGGATCCTCCCCCTGCGCACTGGAAGCTCTTCTGGGAACAGATGTGCGGATGCGCTGATTAATCAGAACATTGCGTCACTGTTGACCCACACAAGCAAACGGCGCTGATAATCGAGAGTGTGTGCGTTTGTGTGAGATGGCCTTGTGTCGTCACCGTTTTTGTTTACCTTGTTGATACCCTAAATACGCATCCGCTTGCTCCATTTGCAGTGAAGAAGGTCCAAGAGGAGGGCAAGTACGCCATCGACTCGCCTGGATCGCCCAGCCAGTATGACATCCCGCCGGAGGACTATGCCCTGCGTAAGTGCATTCGTTTTGAAGGGGCATCTCCTCGTATACCCATCGCATTTCCCACAACTCATCACATTTGATCCTACAGGCGAGCACGAACAGCGCGCCGTCATCGATCCACAGTCCATCAGTGATCCCCAGGTGATAAAGCTGCAGCGCATCCTCGTCGACTGGATCAACGACGAGCTGGCCGAGCAGCGCATAATTGTTCAGCACCTGGAAGAGGACATGTATGACGGCCAGGTGCTGCACAAGCTGTGGGAGAAGCTCACCGGCAAGAAGCTGGACGTTCCTGAGGTCACCCAGTCAGAGCAGGGCCAGCACGAGAAGCTCAACATCGTCCTGAAGGCCGTCAACCATGTAAGTAGTAGTAGTTTATGTTTTGCAGGATTTAAAAATCGGAGTTACCAACATTTAATTATTTATCCATTCTTAAAGTCGAATAGAATAATTTTTATTCGATTTACAATTAACATTCCCTTTTCTCGCGATATAAGAATAAATTTTATCCTTGCCCACACCCACAGACACTCGGCTTTCACCAAAAGATTCCCAAGTGGTCGGTGGCCAGCGTGCACTCGAAGAACATTGTGGCCATATTACATCTGCTGGTGGCACTGGTGCGTCACTTCCGGGCGCCCGTTCGCCTGCCAGAGAACGTGTTCGTGACCGTCGTGATT

 

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