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CRIMIC CR00559

Gene Sod2

CG8905

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:16773696..16773696	53D8	FBti0200586	80593

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	GCAGATCCATGTCGCAGTTGGTTATC	GCACAGCTGATTTGCTAGAGATGGCA

Images

Construct Design Sequences

sgRNA sequence	ATTACATAAATAGCAACAAATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GAGGGACGCACGTTCTTGTA	CGACCCTGATCTTGAGGACA	GGGTCGCCATTTGTTGCTAT	GGCAAATCCATTGGATCCGC

 

Amplicon 5'

Amplicon 3'

GAGGGACGCACGTTCTTGTACTGCAGATAGTAGGCGTGCTCCCAGACATCGATGCCGAAGAGCGGGATCAGGCCGGTGGAGGCCTCCAGGGGATCCTGGTTGGGCAGGGCGGCCAGTTGCAGTTTGCCCGACTTCTTGTTGAAGCCCAGCCAGCCCCAGCCGGAGCCCTGGACCGCCACGGTCAGCGTGGTCAGCTCCTTTTTGAACTCCTCGAGGCTCTTCCACTGCGACTCGATGGCCTTCTTCAGATCATCGCTGGGCTGGGTCTTGTTGGGCGAGAGGTTCTGCCAGAAGATGGTGTGGTTGATGTGGCCACCGCCATTGAAACGCAGGGCAGGAGCCAGCTGAATCAGCTTGGTGGTGTCGCTCTTCGACTTGGCCTCCTCCAGCTGCTCCTCGGCGGCATTTAGATTGTTGACGTAGGTCTGGTGGTGCTTCTGGTGATGCAGCTCCATGATCTCCCGGCAGATGATAGGCTCCAGGGCGGCATAGTCGTAGGGCAGCTTCGGTAGGGTGTGCTTGCCACGCACCGCCAGGCTGCAAAGGGTTAAGTGTGTCTTGTTTTATAATGTCCTCAAGATCAGGGTCG

GGGTCGCCATTTGTTGCTATTTATGTAATCAGCCGGCATCGATTTGGGCATACTTTTTTTCGCAACTTACCTTGCAGTTTGCGAAATTTTACGGGCCACGAACATATCTGCGTTTTATTCGACTGCTAATAAGGCTAAGTCCCGATATCAGATTTTATATCAAATAAAACAAGGCTTACGTTCTCGTTATTTCGCTTGTTCTATTTGAACAATTTACTGCTAGAGATGAGCAGATGAGAAAATATCGAAAGACCCCAATCAGTCAGTGATGTGAGATCAACTTATATATATTGAAGTTAAATAGTAAAACTAAAAGAAATTAAAAACTATTTTTAATTGAAACATTGCACTGAAACATATTCAAATCATATTGAGGATTTCTTAAATATTTCTTATGTTTAAATACTACTTTAGTGACTATTAGCATATTTTAGCTGCTTACGTATCGACTGCATCCATTCGATTGATACTTGAATTAATCGATTTTTGGCCTCTGTATGTCATGGCGCTAAATTGGAAATAAACCATGAAATTAACGTCATAAGTTTAAAAATCCGACTGGAACACAGCACACAACATGTCTACATTTCAAATACCTTCCCGAATCAAAATCGATATAACAAATAAACGGGCACAGAACATTCTTCACAAATATCTACATTTACGTAAGTTGCTTAAATAAGCTAAAGATTTTATGATTTGACCATGATTCGTTCAGCAACCTCTCCTGGAAACATGAGCAGCGAGGTAGATAGTAATTCACACCACGCCGCAGTGGTTATTGACAACGGCTCTGGAGTATGTAAGGCTGGATTCTCCCCAGAAGATACTCCCCGTGCTGTCTTCCCGTCGATTGTGGGCAGACCTCGTCACCTGAACGTCCTGCTGGATTCCGTGATCGGTGATAGTGTTATTGGCGAGGCGGCAGCCCGAAAAAGGGGAATACTAACCCTAAAGTATCCCATCGAGCACGGAATGGTCAAGAACTGGGATGAAATGGAGATGGTTTGGCAGCACACGTACGAGCTCTTGCGGGCGGATCCAATGGATTTGCC

 

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