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CRIMIC CR00561

Gene UK114

CG15261

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:15274503..15274520	35D1	FBti0199414	78952

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	short band	GCTCGAGCTACAGAAACAACAAAGCG	CGTGCGTCGAAATTAATTAGCCGCAG

Images

Construct Design Sequences

sgRNA sequence	ACGATGCCGATTGCCTTATCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAAACACGAGTCCGGGAGTC	GCATCGTTAGCGCCTTATCG	CGATTGCGATCAAAAGGCCG	TACTGCCAGGTTTCACGCAA

 

Amplicon 5'

Amplicon 3'

AAAACACGAGTCCGGGAGTCGGAGTCCGTAAAAGGCGAAAAGAGCCTGAAAGGCAGCCGCATCAATATGGAGCATACACTGTAAACAATTAGGGGTGGCAACAGAAGTGGTAGATACACGTTTAACTGCATTGCAAATCAAAACTTTATTTGATGGCCAATACTTACGCTGCTTATTTATGCTGGTTTTCTAATCAATTTAAGGTAAGCACTAATATATTTCTGCAGTGTATGGGCAATGTCCTACAACAGCAACAACAGCTCAAAGTCGGCATTATGAATAACTTTTAAATTTGAATGTGAAATAATCGCTTTGTCTGCGCGGGCTACCGTTAGCAACATGCTGCTGTTGCAAGTGTTGCTGGAGCTGCAGTGGCTGCTGTTGCTGCCAGTGATTTATGCAGCAACATTTGATGAAATATAATACAAAAGGCGCACAGCAGCCGCAGCAACAACAAAGATAAATTGCCGCACGCTAAATTGTAAGCTATATAAGGTTTAAATGCAAAGTGTGTTGTCCGTAGCCTGTTGCACGTTGCATGTTGCATGCTGTTGCTCGCCGCTTGGCTGCTGCATGTTGCAACAAATGTTGCTCGGCATTTATGGCTTCATTTGACAACAGTATTGAAATCACTACGTTTTCCAGCACTTTTCCTACGCTTTTTTTTTTATTCATTTGGTTCTACTTTTCTACTTTTCTCACAATTTATTAGACATTAGTTGCTATTGCACGGTTTTCGTCTCCACAGATCCGGTCAAGGCAATGCACTCGATCTCCACCAGAGCATCCATGGGCAGCTTGGCCACCTGGAAACAGCTGCGGGCCGGGAAATCCTTATTGAACACTGAAACGGCAGATAAGGAGACATAAAGCATATAGTTGTTTTTGGAAATATAGCATAGCTGCCATAAAGTGTTTACAGTTAACAATAAATGGGCGATAAGGCGCTAACGATGC

CGATTGCGATCAAAAGGCCGGTGTATACAGTAGGTATTGCATTATAAACGACCCTTATAAATAGTGCTTAGTATTCACAAGCCATATGCCTAGTACTTACCCCGCTTGTAGACCTCATTGACTGCCCCGAAATCGTTGAGATCCTTCAAGAACACTGTGTTCTTGATGACTTTGTCCACTCCGGAATCGGCCGCCTTTAGAACAGCCTCCAGATTCTCCAATGCCTTCTGCGCTTGTTCAGTGGGTCCTCCAGGAACCAGTTTCATGGTGTCCTTGTCCAGACCCAGACATCCGGACACATACACAGTACGATCGGCCACCACAGCCTGGCTACATAATTGTAAATCACAATGAAATAGAAGGTGTGTCCAGCAGCTTTTACAGGTACAATTGTTTTCTTACTTGTAGGGAGCCACTGGCTTGGCAGCATTTGCAGTGCTGATAAGTTTCCTCACAATCGTCGACATTTTGATCTCGAAATACGATACTATATAAGTGGCAAGAACAAGCAAAGTCAATTGCAGCTGCTAAAAGTGAACTGATCGAAAGCTTTGACTTGCGTTCAACAAGTGACTTTTGTTATCAGCTTTTGCTTTTCACAAGCTCATTCGCTTTTTCCCGCGCAAACAGGTGCCATCAGCTGATTTGATGAACAGCTGTTCAATGGATCTGTTATCATAATGTTAATATAATGTCAAATATAATGCTCATTCTGTTGCAAATGTAAAATATTGGGTAATATAATGGAAAAAATTATATTAATGGGTGAATTGCGAATGCAAAAAACGCTTATTCCACATAGCTTAGATATTTGTTTCATTTTTGCTTTGCGTGAAACCTGGCAGTA

 

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