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CRIMIC CR00562

Gene Vha68-1

CG12403

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:13003403..13003416	34A4	FBti0199604	79277

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	ACACGGATGCAGGTCCTTTGCTTTCT	CACCAGCTGAACGATCTCAGACAGAT

Images

Construct Design Sequences

sgRNA sequence	GCATAATGAGGAAGATTATGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGTAGATGGAGGCCTCACGA	TCCTCATTATGCTGCTCTCT	ACAGGAGGAGGGGGAAGAAA	CTGGAGCCCTCGACGATAAC

 

Amplicon 5'

Amplicon 3'

TGTAGATGGAGGCCTCACGAGCAGCCACCGGCATGTTGGAGGTGTTGGCCACCAGAGCAGTTCGCTTCATAATGGACTCGGTGACGCCATCTATCTCGCAGGTCAGTTCGGGAAAGTCACGCAGTACCTCAGACATCTCGTTACCGCGCTCGCCGCAACCGACGTAGATGATCACATCAGAGTTGGAGTACTTGGACAGGGCCTACGGGTGGTTGAAGTGCAGATTGGAAGGGATGGTCATTTAATGAGATGTAAGATGTGGATTAAGTGAATAATTTGCAACTTGCCTGCGAAATGACGGTCTTGCCGCAGCCAAAGGCACCGGGGATGGCAGTGGTGCCGCCCTGTACGCAGGGGAAGAGCGAGTCAAGGACGCGTTGGCCCGTGAAGAGCGGATGGTTGGCTGGCAGCTTCTCTGTGACGGGACGTGGCTGCCGCACTGGCCAGACCTGCAACATGGTGTGCTTGGTTATCTCGCCGTCGAACTCCGTCTCCAGGACAATGTCCTCCAGGTTGTAGTTGCCCGCGGGGGCAATGTATCGAACGGTTCCCTTAGCCCTGGGTGCCACAATCATGCGCTGCTTCACCAGCGTGTTCTCGTGTACCACTCCATACAGATCTCCTCCGGTGATGTGGGATCCCACCCGCACATTCAGCGGATTAAATTCCCACATCTCCGAGCGCGACAAAGCAGTTGTGTTGACACCTTTGGGTATATAGATGGAGTTGGTCATGACACCAATGTCCCGCAAAGGACGTTGGATGCCGTCGAAGATGCTGCCCATAATGCCGGGTCCAAGTTCCACGGAAAGAGGTTTGCCGGTACGCAGCACCGGATCGCCGACAGTCACGCCAGAGGTCTCCTCGTACACCTGGATGGTGGCCATGTCGCCCTCCAGACGGATGATCTCGCCCACCAGCTCGTAGTAGCCGACGCGGACCAACTCGTACATAGCTGATCCAGACATGGCCTCAGCGGTGACCACTGAAGTGGATTTAAAGTTGTTGAAGGAGGAAAAGCAGGAGAAATATGATTAGCAAGGAGTGGAGAGAGCAGCATAATGAGGA

ACAGGAGGAGGGGGAAGAAAGCATACAGAAATATGAAAAGGAGAAGGATAATAAGGAAGGATAAAAAGGAAGATGTGGAGGAGTATAATTAAGAACAGAAGAAAGGCAGGATTAAGGATTACAGAACTTGGGGGTCAGCGCATGAAGGACGCTGGTGACGTCGCTTACCTGGTCCGGATACCGCGTAGACACGGCCATATTCCGACTCGCGCTCCTCGTCTTTGAATTTCCTCAAGTTGGACATGGTCGCGGTTGCTGCTCTTCTTCTTCTGCTTCGGTAACGGTCAAATCGTTCGTTGGCTGTGGGAAAAAGTAACCGTTTTTCTTATAAACAAAAACACGCATTTTCCATGCCCCCGAATGAGAGAGCGTTTTCCGCGAGGAAACGCATAGGTGGCGCTGATGTGCAGCAGCAGCCCCCAGTGAAAAGCTTCACACCCCCGCAGCACAGCATCGATTTTCCAATACAATTTTCACACATGCCACCAACGGCAAGGTGAATTTTCCACCTTTTACCCATCACAAAAGCAATTTTCACACTTTCTTTTCATTCAACACCTTGTGGTCAGTGGTTTTGTGGCGTAAAAACTGCTTTTTCAGCTGCTCTAAAATTAAATAAGCTCACATTTGTTTCAACAGCTCTTATGAAAATTCTCTTGCTTTTTTGATTTCACTCTAGTATTGATCTATCGGTTATCGTCGAGGGCTCCAG

 

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