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CRIMIC CR00565

Gene ND-MWFE

CG34439

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:13158889..13158901	49F4	FBti0200588	80595

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CAACAAATCGCTGAAGCTCTTGAGGC	CGGCAGCCATGGCTGTGTTTTATGTA

Images

Construct Design Sequences

sgRNA sequence	GATTGGTGCACAAACAGCTGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACTCGACAATACCGCTGGTG	AGAATTTCGGGCTGCTGGG	AGCTGTTTGTGCACCAATCG	TTTCCTGTCACGAGCACAGG

 

Amplicon 5'

Amplicon 3'

ACTCGACAATACCGCTGGTGGCCATCCTGCTCTCCGCTGATTTGTCGCTCTGCTCTTTGTTGGTAGTCTTCTTGCTGCTATTGCTGCGCTCAGAACTCTCTGCGAATTCCTTCGTAGCTGTTCAATGAAGAATCGGATTGGTAAGACCGAGATCTGAGTGCAAAAAAATGGTCCAGCTGGATGTACGAAAAGTGCATTGGGAAAAATACCAACCCGAATAGAGCGTAAAACGGTCACGCAGCTTTACACTGGAATTTGTTTTCTATATACTTTCGAGCTTTGAAATTGATTATAAACATTGCGTACATTTATCAGCTCTACTAGATATATCAGATCTATTTACAAATTATTTAAGCCAACTATTGGTCAAAAACTATTTTCAAATCAACATTGACGTTTTTCTAAACGCAGTAGCAGAATAGAATTCAGTCACCGTTACTTTCTTATCGTTACTGGCCCACCTCTAGTTCAACATTCAAGTTTTTGGTATATTTTGTAAGGCTACCTGTATATTGGAACGGACTATCCACGGTCACACTGGGTGTCGTCTTGCAGTAAATATCATAACAATCTATTTCCATTGACAATATGTGGTTCGAAATCCTACCTGGTGCGGTGATCATCACCACGCTCCTCTCGGTGCCCATATACGCCATGTACGGCCTGGACAAGCTGATGATCGGCAATGTGAGTCCCACGCTGGCCTTCTGGCCCAGCAGCCCGAAATTCT

AGCTGTTTGTGCACCAATCGGAATCTGTTAAACATTATTTAACCGCCTGCTTTTCGCAACGAGTTCGACGGAACACTACGGAGTTACTAATTGTTTGCTAATCGTTTGCTCAACAGGCTTTCCGGCGCAACATGGACGAGCGTTTCAGCCGAGTTATGTACCAGCGCGATTTCCGACTGACCGACAATCCCTACAAGATGAACGTAAGTGTTGTGCTATAGTCTGGCCTGCCTACAACTGATTTTTGTGCGGCAGGCCAACCATCACGTTTTTACCCTATTTTTGGCAGCAGTTTTGTTATCTCACAGCACGCACTTCATTATTATGCACGCTCATTTTGGTTTCGTTGTTTATGTTTTTCTCTGAAGTATGTTAAATAGATTGTTTTTGGTGTTATGAACTAGCTTTCTTCACTAATAAAATATTAGTTTTAAAGTTTTCTATATTATAAGGTAATTGCATTTAAAATCTTAGTTTTCTTGGTTAATTTCGTTTTTATTGTTTATTGATCATTGGTGGCGGTAGTTAATGTTACTTTATTTCCTACGAATTCATACCATCCATTTTTTTACGCTTATTCGAAGAGTTATAAGCTTAAATCATTTACAAAAAACCGAACCAATCTTCTTGCAGGGTCTGGATGCCATACCGGATGAGAAAACGAACTAATTTTAATTAAGTAAAGTTTTCCGTTTAAGCTTAAGTGCAAAAGTGAATAAACGAAACGTGCAGTTCGGAAGTGATAGTTGAAATGGTCGTTTTTGGTTTTTCCTGTAGTTCCTTACAGATTTTTTTTTGCTGCCTCAGGTGTAAAGCTTCTTTCTCTATAGGGTCTTGAACAAATTCCCGATGAAGAGGTGAAGAAGGAGGAGAAGGACCCCAATGAGGACAGCGATGATCCCGCAATCGTGAAGAAGCGGGAGAAAGAGCGGAAGCTAAGGGAGAAACAGCTGAAGAAGGAGGAAAAGCTCAGGGAGAAACAGCTGAAGGAGGAGGAGAAGCAGAAGAAAAATTAATATCAATAAAAACAAACATTTCGCCAGTGGAGCTCTCAAGCCGAGCACAGGACTTTCGGCCGCCTTTGCCGCGCATTTAGCAACCCTGTGCTCGTGACAGGAAA

 

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