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CRIMIC CR00568

Gene Gug

CG6964

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:8462860..8462877	66D1	FBti0200589	86331

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGTAGTACCCTTGTGATAGCTACACC	GCGTTGGCTGATTGATCCTTAGACGA

Images

Construct Design Sequences

sgRNA sequence	TGGTAATCCCCCCGGATTGCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTCAATCGTCCGACAGCGTA	TTACCAGCACCCACGAGATG	GCACTAGCTGTCTTGCCTGT	CTTCGGAGGAGTGTTGCTGT

 

Amplicon 5'

Amplicon 3'

CTCAATCGTCCGACAGCGTAAACCCCTAATTTCCCGGCCCCGTCAGCCGTGTCCCCCGGCCACTCCATCCACTCCATCAGCTCCAGCACTCACTCATCGCCACTCCATCGTTCGCACATTACACGGCATTATTTTTATGGATTATTTATTTATGCATGTTGATATGAACTTCATCGCTTTATTGAAAATGCTTTTTACAACACCGCCCCTATCGCCGTTGTCTCTCTATATGTTTGAATCATTTTTTTTTTAACGAATTTTCTCTGTGTGCTTGGATTTATTTGCAGTTGAATTTTCATATCGTAGAGGGCATAAAAAGGCTTAACACTGGACCGGAAGCGTGTTTTTAAAGGGTGATGGCGGCCTCCACTCAAGGAGAAATTCGAGTGGGTCCCGGCCACCAGGTAAACGATGTCTATGTACGTACATAACTCTCACAAATCAGAAATTCAGCTCACGGTAACAAAAATGAAATCAAAGAACTACAAAATTTAACAATTATGCAAAACGAAAATGTAGACCCTATATTTCTTTTGTTTATTGCAAAAGTTGTACTTTATATTCCTTTGTTCTTCCTCTTGGATGTCTTCAACAAAAAAGTTCGTTTACCAACTGTTTTTAAGCGTGTAGCCTTTGAAGGAACAGAAAATAATTAATAACGAAAATAATGGTTCAGTTTCCGTTTCTAATATCAGTCAAGAGAATGTGTAAAAAATTACTTTTCTGCCATATTTCGGAAACTTTAAACAATTCCCTTAGGAAACAAGATAAACTTCTACAAGTTCATGTATTACCGATGTTCTTCCTTTCTGTATACAAATCTTTGCAACCTGAACTGATTAATATTCCATGTCATAAGATTCTATTGTTCCTTCTGATAAGGCTACAGCGAAATGCATTTCTTTAAACATATACATATATCGGTTACACTTAGCATAATCTTGTATATAACGCCTAATTCCTGTAAATAATTTGTAGTTCTAGCGTTTATGCAATTGGCTATTAATAGTTCGAATCCTCGATGAGTAGAAGTTGACCCAGTTCCAAATCCCAACCTTCTTTTCCTGCTTCCCTACCGAAATCCGTTCGAGGAAATTCGTAGCACACATTATACATTATTCATTCATAATCATGCATTAGACTTAAGTTTGTGTATCATCTCGTGGGTGCTGGTAA

GCACTAGCTGTCTTGCCTGTGGTGTGTAGTGTGCGTGGTGTGGTTGTGTGACTAACCCATCAATCGTTCTCCTGGAGTTTTTGAACTAATCCCAAAACTTTTCGTTTGTCTCCTGTGACGACTGAAGGCAAAACTGCCCGATTATAATCCAATCTCAAGCTTCCCCATCGACAAGGAAACCGATGAACGTGAACTAGAGGAATCAAGATGGAGTCCAGGCGTTGTGGCCGATGGCGACTTGTTAATGTTCTTGCGTGCGGCTCGATCCATGGCTGCATTTCAAGGAATGTGTGATGGTGGTTTAGAAGACGGTTGTTTGGCTGCTAGTCGCGACGACACTACAATAAACGCACTCGACGTGGTGAGTTGCCCGAGATGATATATGCATATGGCAGCACTCTTTAATCCGCAATGCCCATTTCAGCTCCACGATTCTGGCTACGATCCAGGCAAAGCTCTACAAGCGCTCGTAAAGTGCCCCGTTTCGAAGGGCATCGACAAGAAGTGGACCGAGGACGAAACAAAGAAATTCATCAAGGGTCTGCGTCAGTTTGGGAAGAACTTCTTCCGCATCCATAAGGACTTGCTGCCGCACAAGGACACGCCGGAGCTGGTCGAGTTCTACTATCTGTGGAAGAAGACGCCCGGCGCGAACAATAATCGGCCACACAGGCGACGCCGCCAAAGCGCCCTGCGACGCAACCGTGTCACGCGGGCCAACAACAGCAACAGCAACACTCCTCCGAAG

 

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