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CRIMIC CR00571

Gene CG10353

CG10353

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:11882103..11882103	10F3	FBti0199417	78955

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TATCCCGAATCTGAAATGGACGAGCG	CGCTAATGGGTATCAGCATTTGCGTG

Images

Construct Design Sequences

sgRNA sequence	CGTCCGCCACGGTGATAGGGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGCGCAGGTATATACGCCAT	GTGGTGACCAGGGGGAAATG	TGGTCACCACCCTATCACCG	CGCCGATGTCCTTATCCCTC

 

Amplicon 5'

Amplicon 3'

GGCGCAGGTATATACGCCATCCTGGACGAGCTTCTCGATGCCCAGATTGTCGGCCGTCTCCTCGCCCTCCACGAAACGCTGGCGCTGGAGAATGAAATTGATGATGGAGTAGCGGGTGCTGGCGTCGAGAAAGTTCGGGTGCTCCGTATCGTACAGTTCCAGGTAGTTGCGGGAGAACTCCAGATGGATGCGCGGTTCGCGTTCCGGAAACAGGGCCGTATTCAATTGGACGGAGCTGAAGAGACTTTTGCACATGCGACTGAAGCAGGTCTTGAACTCGTGCGCCGATTCGGCGAAGATTTGATCCTGGCCGGGAATCGGTTTGAGCGGCACCTTAATCTTGAGTATTTCCGCATAGCGATACAGCACCTCCGCCGGCGCATGGATCTTGATGAAATGGACGCGCTGCACCTTGTTGTGCTCCAGCTGCAAGCCCTCTCGCTGCAGATTCGCCTCAAAGATCTCGCATTTGCGCCGATGCTCCTCCAGCTGCGTCTCTCCGTTGTAAGCCAGCACAAAGTCCACGCTCCGTTTGCCATCGTCGAAGCGTCTGTATGTCTGCAGAGATTCCGAGAGATCCGGTAAGAAGCACGTGATGAACAGCGGAAAGAGAGCAACCGTAGATGGAGAGCATTAGTAAAAATCAGTGTGAGTCACTCGAGAGCGATCTTAAAAATATACATTTGCTTATCAACTAGACGGAAACAAAGCGTGTGGGTGCGTGTTTGCCTAATGGCCATTTCCCCCTGGTCACCAC

TGGTCACCACCCTATCACCGTGGCGGACGCCTCCCGCCGATCTCCCGAGCGCAATCCGCGCAGGAGGCAGCAGTTCAGCATGATGACAGCCCGGGGAACCAAACCAAATCGAATCGAACCGAAACACAACCGACGTGGTCGAGACACTACACGCAAATATTGGATTCTGGCCGCTCGGCTGGCTCTACTGCAGATGGCCCAGCTGTTTGCTGCAATCAGTAGCTGATCCAAAGTAACAAAACGCCCGACTTAGATACTAAAGATATCAGTATACAAAATTCGCATTAAGTGTTCATACCCAATCCCGCAAGCAAACTATAGGAAATAGAGAAATATAAACAAACAAAGGAGAGCGTGAAAGAAGGCGGCAAGAACTTGATCAAGTGCAGAATATGTAAATGTGTGTGTGTGTGTGCATCATTACAGCAGCGGGGCAATTGTATGTGTGACTGGTGTGGGTACAGTGGTCGCTCCTTAACGCGAAATCCTCTTCGATTCCCTACTATGAATATGAAAGTTACAGAGCCTCTAATTCAGTCAGTGACTCATAGAATCTGATAATGATCTTAATTCAAAGAGTCTATTTTTAGAAGATCTTCTATAGATTTTTACAAAATATAGCTAAGTACTTGTTAGATTGGCATTTAAAAACATTGTTAATATATTAAGTAATGGGAATTGTTTCGAATTTCGATGAGTAATCAAGGGAGTGCGTAGGAGCAACCACTGCACCAGTGACCATCGAAACTCGTGTGTGAAAGCTGGAATGAGCGATCCGGCAGATGGTTGAAAGTGACTGGCGATTGGCGGATGAGATGACTGACTGACTCGGCATCCACGCAACTGCAAGACAAACATGGTGGTGAGATCGATGGACGTGCGAGGGATAAGGACATCGGCG

 

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