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CRIMIC CR00572

Gene Oaz

CG42702

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:14461250..14461268	51A4	FBti0200590	80596

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ACGTTTGCCACACTTCCATCATCACC	ATTTTGGGCAAGTCCAAAGCCAGTCG

Images

Construct Design Sequences

sgRNA sequence	CCGGCTTTGATCCTAGCGCCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTTGCCCCTTTGGTGACAGT	GATCAAAGCCGGATCGCAAC	TGGTACGGTTCGGAATTCGG	CCGCGGACTATAGTTGAGGG

 

Amplicon 5'

Amplicon 3'

TTTGCCCCTTTGGTGACAGTCTTCTAAATACTTAGGACACCTTTAGGCACTCAGGGTGTCAGGACTTTGATTGACAATCTGCGGTGGTTCCATTGATTTTCATTTCCATCGAGTGAATAGGAACCAAACTGCGATTTCAATTTGTTAATTGAAAGCGAAAGTGGCATAGCTCTTAGATGTAAAGGGTTGTTTAAGTGGAAATGTCAAATCTAGCATGTACGCAAGGCTCGTTTCTCCTTTGAACAATATATGTAAAATAAAGGTACGTTCTCTGAAATCTTCCAAATGAAACAAATTAGGATCAAGTGAATTATTTAAAAATTGCAGACCTATATCATTTATCTAACACTCCTTCGGCAATCTCTAAAACAACATCTAAGACAACCCGAAGCTTTGAGCCTTTGTGGCCACCGCACTTCACCCACATGCCCCCTCATCTCCGCGAGCGAAAATTCTAATTCGGCTTAAATGCTTTTTGAACTGCTAACACCGCCGCCCATGACATCAGCAACTTGTTAACCCAATTGCAACTGCTGTTGAGGACTACCGAGCAGCGATCTTTATCGTCCACCGGGGTCTAACATGTTGCGATCCGGCTTTGATC

TGGTACGGTTCGGAATTCGGTCTTTCAGCTTATGGGATGTTGATGGCGATGGTGATTGGAGCGACAGTTTAAAAAACCATAACCAACACGAGAAGTTGTACGCTCTCTGTGGATCTGGATGCGACTAACCCGGCTAACTCATCATATATTGTGCAATTTTAAGGCTTTCCAAACTGGGGGGCTGCATACAAATGGGCTAATTTTTTTTTTCGCCGCTCTCAAAGTTCAAAGCCGGCTGCCGCTAAAAGAACCCAAAAAGCAAACCGGTTGCGGGTGTAGTTTAAGACCCAACCCCAGCCCCAGCACCTGCCCCTTTTCCAGCCCAACCTGCGAAAGAAAAAAAAAACAAAAAAAACAGTAAGCGGCGTCGCGCATTTAGATGAATTTTGTCCACAAGCCACGAGTCGCAAGTGAAACACCTACTCGTCTGGGCCTGAGTCCGAGTCGAGTCAGTCGGTATGCAGATTCAGATTCAGATTGACGCATCTATAAATTCAAGCCAGGAGTCGTCCAAGTCGTCGTCGTAGTCGTCATCGTTGTCATCGCAACTTTTAACTGGGGTAGACGGACAGTTGACGCTGCCTTGTTGTCTGGTGCTCTTCTAAGAATTTCAAATAGCCCAGTGGGCCTACGGCTAATTGCTCAGATTATCATCATGCTAATGGGATTTTCCGAATATGTGTATATTCATTACAGAGCCACGCTGAACACCTGCCTTTCAAATGTGAATACTGCTCCAAGCTCTTCAAGCACAAGCGATCCCGAGATCGTCATAAGAAACTGCATACCAACGAGCGGAACTACAAGTGTCCGCATTGCGAGGCAGCTTTTTCCAGGAGGTAAGTGAGGGAGTCTGGTTTAATATGTGACCTATTAACTAAGTCAATAAATTTCCTCTATTTCATCTTTCTCTAGTGACCACTTGAAGATCCACATGAAAACCCATGACATACAGAAGCCCTTCCAGTGCAGCATGTGCAACAGGGGCTATAATACAGCTGCTGCTCTAACCTCGCACATGCAAAAGCACAAGAAGAATGCAGCCATCCTGGCAGCCGGTGGCAATCCGAATGCCCTCAACTATAGTCCGCGG

 

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