CR00574

CRIMIC CR00574

Gene CG42255

CG42255

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:11813792..11813794	68E1	FBti0200591	80597

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	TTGTCCATCCCACGAATTTGTGACGC	TAGCACCATGTTCCGGTTTGTGCGTA

Images

Construct Design Sequences

sgRNA sequence	GTATGGGGACGTCGAGATCTTGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATCCGGTAATAGGCGGCATG	AATAAGAGAGCCAAAAATCCGAC	ACCAAGATCTCGACGTCCCC	CCGCCACCGTGACTATCAAT

Amplicon 5'	Amplicon 3'
ATCCGGTAATAGGCGGCATGTTCCACCATTTAGGCAGGGACTTGGGTGGCACGCATCCGATGAGTTGCACACAACGCCGGAATAGCCAGGCTGGCATATGCACGTGGTTCCCCTTCCGTTCTGGACGCAAGTACCGTTGTTCTGGCAAGGATGCTGGTCGCAGGGCAGTCGACTGGAGTCCGCGCTGCATCCATCGGCACCATAGCCATGTCCAAAGCTGCCCAATGGGCAGGTGCACACCACCATGTCGGATACATACTCGCACTTGGCCAGCGGATGGCAAATCCCCTCATTGTTGCACGAGTTTGAATCCGATGCTGTGCATGTTCGTCCGTCGCCCGTCCAACCGGCTGGACATCGGCCACAGCGATGTGAGCCCTCTGTGTTGGTGCAGGTTACCCGTGGCTGGAGGCTACAGCCGCCATTATCCTCGGAGGCACATTCGTCTATATCGCGGCAGAAGCGCCCATCACCCGTGTATCCCGTTGGACAGGCTCCGCATCGAAAACTTCCCGGCAGATTAATGCACTCATCGTGGCAAGGATTCACGCGCGGCTCACACTCATCCACGTCGCGGACGCAGGCACTTGGGCTGGCTGAGGTCACATTGGCATCTGCCCAGGTCCATCCCTGATCGCAGATGCACACATAGCCAGCGGAATTGGCCGCCTGGATGCAGGTGCCATGCTCGCCGCACAGCTCCCGGGAGCCGCCGAACAGACAAGTGTTGTGACGAAGGCGACAGTGGGTGCCAGTGAATCCATTGCGGCAAACGCACCTAAAACAGCAATTTTGCGGTACATATTTCATTTCCTGCACGTGGAACTTCTCGTCAACTTACCGGTAACTTCCTGGAGTGTTAATGCACTGACCATTGTTTAGACACCCATCGAGATCTGTTCCGGCCAAAGTGAAGCACTCGTTTACGTCGTCCTCACAAGAGTCACCCTAAAAATAAACGAATTTACTAATCCCAATAAACTTCTGTGCTGGAACTATGTACATATGTATGTACATGGAGTGATAATTCTAACCTCAAACCTCTATAACTTCTTTAACCATGAAACGCCCCAATAACTGTATTCAAAAGTCAAATACAGTCGGATTTTTGGCTCTCTTATT	ACCAAGATCTCGACGTCCCCATACGGACAAACGGACAAACATGATCAAGAATTCTTCATACGGTGACTTTTGGTAACTAACTTGCATTGTAATTAAGCCAACGCATTTCCAGTCATATATGTAAGTTACTCTTTCGTGTGAAAATACGCCCCATAATCTCTTGAGAGACGCCCCCGCTTACTCACTTGCCAGCCCGCCGGGCACTCGCACTGGAATCCCTTGTAGGCATCGTGGCAAGTTCCACCGTTTTTGCACGGATTCGATAAGCACTCGTTTCTGGCCAGGTTTCCCGCGAGGGTCTGCAGTATTCCTTTGACGCGTTCCACGCGCTGCAATTGCCGGCGCAGGATGCGTTGATTGAGCCCACTTCTTTTCGTGGAGTTTCTCGCACTGAAGATCCTTCGCTGAATCCTCGTCATTTTAAGCTCCACATCGTGGAACATATCCCTAACGGCGTCCACTTTCAGGGGCTCCCTTCGCGCCGCCTGACGATCCGCAATAATCCTTTGACGCCGCCGAAGCACCGTCATCATGTCTACATCATTGATAGTCACGGTGGCGG

Amplicon 5'

Amplicon 3'

ATCCGGTAATAGGCGGCATGTTCCACCATTTAGGCAGGGACTTGGGTGGCACGCATCCGATGAGTTGCACACAACGCCGGAATAGCCAGGCTGGCATATGCACGTGGTTCCCCTTCCGTTCTGGACGCAAGTACCGTTGTTCTGGCAAGGATGCTGGTCGCAGGGCAGTCGACTGGAGTCCGCGCTGCATCCATCGGCACCATAGCCATGTCCAAAGCTGCCCAATGGGCAGGTGCACACCACCATGTCGGATACATACTCGCACTTGGCCAGCGGATGGCAAATCCCCTCATTGTTGCACGAGTTTGAATCCGATGCTGTGCATGTTCGTCCGTCGCCCGTCCAACCGGCTGGACATCGGCCACAGCGATGTGAGCCCTCTGTGTTGGTGCAGGTTACCCGTGGCTGGAGGCTACAGCCGCCATTATCCTCGGAGGCACATTCGTCTATATCGCGGCAGAAGCGCCCATCACCCGTGTATCCCGTTGGACAGGCTCCGCATCGAAAACTTCCCGGCAGATTAATGCACTCATCGTGGCAAGGATTCACGCGCGGCTCACACTCATCCACGTCGCGGACGCAGGCACTTGGGCTGGCTGAGGTCACATTGGCATCTGCCCAGGTCCATCCCTGATCGCAGATGCACACATAGCCAGCGGAATTGGCCGCCTGGATGCAGGTGCCATGCTCGCCGCACAGCTCCCGGGAGCCGCCGAACAGACAAGTGTTGTGACGAAGGCGACAGTGGGTGCCAGTGAATCCATTGCGGCAAACGCACCTAAAACAGCAATTTTGCGGTACATATTTCATTTCCTGCACGTGGAACTTCTCGTCAACTTACCGGTAACTTCCTGGAGTGTTAATGCACTGACCATTGTTTAGACACCCATCGAGATCTGTTCCGGCCAAAGTGAAGCACTCGTTTACGTCGTCCTCACAAGAGTCACCCTAAAAATAAACGAATTTACTAATCCCAATAAACTTCTGTGCTGGAACTATGTACATATGTATGTACATGGAGTGATAATTCTAACCTCAAACCTCTATAACTTCTTTAACCATGAAACGCCCCAATAACTGTATTCAAAAGTCAAATACAGTCGGATTTTTGGCTCTCTTATT

ACCAAGATCTCGACGTCCCCATACGGACAAACGGACAAACATGATCAAGAATTCTTCATACGGTGACTTTTGGTAACTAACTTGCATTGTAATTAAGCCAACGCATTTCCAGTCATATATGTAAGTTACTCTTTCGTGTGAAAATACGCCCCATAATCTCTTGAGAGACGCCCCCGCTTACTCACTTGCCAGCCCGCCGGGCACTCGCACTGGAATCCCTTGTAGGCATCGTGGCAAGTTCCACCGTTTTTGCACGGATTCGATAAGCACTCGTTTCTGGCCAGGTTTCCCGCGAGGGTCTGCAGTATTCCTTTGACGCGTTCCACGCGCTGCAATTGCCGGCGCAGGATGCGTTGATTGAGCCCACTTCTTTTCGTGGAGTTTCTCGCACTGAAGATCCTTCGCTGAATCCTCGTCATTTTAAGCTCCACATCGTGGAACATATCCCTAACGGCGTCCACTTTCAGGGGCTCCCTTCGCGCCGCCTGACGATCCGCAATAATCCTTTGACGCCGCCGAAGCACCGTCATCATGTCTACATCATTGATAGTCACGGTGGCGG

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