CR00575

CRIMIC CR00575

Gene amd

CG10501

Information

Donor Location Cytology FBti Stock number
pM37-p02L:19112448..1911245437C1FBti020059286332

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band OK GCTCAGGAGATAGGCACTCAACGATT ACTGTTTGGCCAACTCGATGTGCTTG

Images

Construct Design Sequences

sgRNA sequence GTGAACAACTCTCTTCGGTCCGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
GGCGATCGGTTTCAGAGTGA TGGCAACCTGTTTGTCGAGT CCGGACCGAAGAGAGTTGTT GCTCCTCTCAGTGTATCGCC
 
Amplicon 5' Amplicon 3'
GGCGATCGGTTTCAGAGTGAACCTCAGGCAACTTGGAGGAGCATCAACGGATCGGGAACTGAAATCGAGTTGGGCAAACAAATCAAAAACGAAAACGGGGAAATAAAACCAAAACAAACAGAACGTAAAAAGTGCAAATAGAAAACGATATCGCAACATTGTCAGCGGTATGGATGCCAAGGAGTTTCGGGAATTCGGCAAGGCCGCCATTGACTACATAGCCGACTATCTGGAGAATATTCGGGATGACGACGTACTGCCCAATGTGGAGCCAGGCTATCTGTTGGACCTGCTGCCCACAGAGATGCCGGAAGAGCCCGAAGCGTGGAAGGATGTCCTCGGCGACATTAGTCGCGTCATCAAGCCGGGACTGACCCACTGGCAGTCGCCTCACATGCATGCCTACTACCCCACCAGCACCTCGTATCCCTCCATTGTGGGCGAGATGCTGGCCAGCGGGTTCGGCGTCATCGGATTCAGCTGGGTATGTTGGTTTATGGTGAAATCTGCTGCTGCTGCTGCTGCTGCTTCTGGCCCATTGTTTTGGCCGGCTGAATGGGCGCTCATTGTGCGCGGTGGGTGAAAGTGAATCCAAGAACTCGACAAACAGGTTGCCA CCGGACCGAAGAGAGTTGTTCACACAAATCAATCGGCAATTGTCACCAAATAAAAGTTATTGGGCAGCAGACTCACCTTAAAGGCATACAAATAAATTTAAATGTATATAGAATTGATTAGCCAAACCCAAACTAGACGTTTCGCAAACAATATTTGTCATTGCGAACAAAGAAGTTACCACCGAACAAAAACTTAGTGAAATAAACCCTAGTTTAAATTATAATATATTTGTAAAAATTACTATATGTATGTATTCCGGATTTAATAGTGTATTACAAACGGATGGAGTTATCTTCAATGCATAATTTCTTACATAATAATTCGTATAATCCCCCACAGATCTGCAGTCCCGCCTGCACAGAACTGGAGGTGGTGGTCATGGACTGGCTGGCCAAGTTCCTGAAGCTGCCCGCACACTTCCAGCACGCCAGCGATGGACCAGGAGGCGGGGTGATCCAGGGATCAGCTAGCGAGGCTGTGTTGGTGGCTGTCCTAGCTGCCAGGGAACAAGCTGTGGCCAACTACAGGGAATCGCATCCGGAGCTGAGCGAAAGTGAGGTGCGTGGCCGCTTGGTGGCCTACTCCTCGGACCAGAGTAACAGCTGCATTGAGAAGGCTGGAGTCCTGGCTGCCATGCCGATTCGATTGCTGCCGGCTGGAGAGGATTTCGTACTTAGAGGCGATACACTGAGAGGAGC
 
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