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CRIMIC CR00578

Gene santa-maria

CG12789

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:7447440..7447443	28A1	FBti0200593	80598

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	ACCAACCAGAACACAACTGACACGCT	ACAATGGGCAGCACCTTCAGTTGATC

Images

Construct Design Sequences

sgRNA sequence	ACTAATCGAATGCAGCTGGCCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TAGTTCGGCACATATCGGGC	AGCTGCATTCGATTAGTGGCT	CCGGGCTAACACTGGAGT	GTGTCGGGGGAAGCTTTGTA

 

Amplicon 5'

Amplicon 3'

TAGTTCGGCACATATCGGGCGTAAATAGACCAACACTATCGCCGGGCTTCAGATGCTGTGGCTGGAACTCCCCGGCGGATCCATTGGTCATACCGCAATAGGAATCAAAGAATCCAGTGTTCTCCTGGTAATTCCACGAGTGCATCTGACCCAGTTTGGCCAGTTGATCGGCGCCAGTGAAGACATTAAAAACTCCGGTAAGATCGGCACTTCCATTGCGCGTATAGAACCAACCGAACTTATCGAATGGAACCTTCACCTCATCACCAAAAATGGGCATGGCCATGGCCACGTCGATCATTGCGTCATTGTAACCGGTGAAGAGCAGCTCATCGATCGATTTTTGGACAGACATCTCGGCACCGTACATCTTTAGTCCCACATCGACAAGGGAGCGCTTAACTGGTGGCCAATATTTGGCTGTGGCGGCTGCAGACTGAAATGGAAATGTATAGTTATAGTTATATTATGTAATAAAAGGGAATAGGATACTTACCAGAGCCACTGCATTAAGCGTGGTAATCTCATCGTCCAGGCTGCCATTACTGCCCGCCGCATCAAAGTAGAACAAGCTGCGCCTGCGATAAGTGACGGATGCATTTTCTGGATGCCAGTGAATGTCCACCTTATCGGGTCGCTCGATAAAACGATAAGGACCCACCTGCTCCAGAATGGGCTTTGTGGAAAGGTTGCCGAAATCCTCCGGATTCGTCCAGTTGTACAGGTAGATGTCCAGACTGAGATCAATTGGCGGACTCTTCCAATTTTCATAGACACGTGTGTCGGGGGCCAGAGCCATTTCCTGTCGAAGAACAGAAGCCGCAATTGGTATTAATTGAAAATCTCAACGAGTTTTCCTCGATCTTTGACTCGCGCCAATTGAGAGGCGGCTGTTTTTCTTTTGACTTTCAATTGGCTTTAGCCACTAATCGAATGCAGCT

CCGGGCTAACACTGGAGTGAAGTGAAATGAACTTAACTGAACCTCTGTCAATGACTGGGATTACCTTGTGCATGATCCAATCGAACAGGTCCACCCAGAACATGCCGCACAATATGCCAAATAGTCCCAGGCAAAAGCCGAAAATGCCGATAATTAATTTGCGATTACTTTTTTGGCCCCACATTGCGCTGTTTTGTGTTGGCATGGCTCACGCTGGGCGATGGTGTGTGGATACCTAATGGTGGCTGTAATGATATGCAAGTCATTGAAACATCAATGAAAATGGATAAATTGATAGTCTATTTGCCAACATATAAAAATGGATCAAATGAAAGAACTCATAAATGTAGTGCAAGTTGAGAATGTACTGATAAGTTTTCTATTATAAAATCATTATGAACAAATCATAATACAATAATTTAAAATATAACTTTACACAATATTATTTTGTGAACTCATGTTCATGTTGCCTACTTTCAGGCAGTGCTAGTTAAAGAATAAAAAAAATGTAGAATCAAATCTCTTATTAACAGATGCATCAAGTCACATATTAATATAAGTATGGCGAACAAAGAAATCAACAAAGAATTGAATACAAATAAAATGCAAAAAGCGCAGCATTTTATTGAGTGACGAATTCCCATAGCGTCTATTTTTATCAATATAATTGAATCAATTTAAGCAAAGCGTGACTTTCAATTGCTGTTTACACTCATGTAGAATATACTAAGTCCGGTAAGTATATGTGTACATATATACAGAACTATAGTCGAATCCCCTATCAGTGGTGATAATTACAAAGCTTCCCCCGACAC

 

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