CR00579

CRIMIC CR00579

Gene Cyp6v1

CG1829

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:20658941..20658949	19E1	FBti0199418	78956

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AACCACATTGTTCGAAAGCCCCGACT	ATTGGATACTTGCGCAGAGTCTCCTC

Images

Construct Design Sequences

sgRNA sequence	CCAGGTCCACGACACACGTTCGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACTCCACGAACATCCTGCTG	GTGTGTCGTGGACCTGGG	ACTCTTACACCGACCAACACA	TGTGAGCCGATCTCCGATTG

Amplicon 5'	Amplicon 3'
ACTCCACGAACATCCTGCTGGCGATAGTGACGATCCTGACGGGCGTGTTCATCTGGTCCCGGCGCACCTACGTCTACTGGCAGCGCCGGCGGGTGAAGTTCGTCCAGCCGACCCACCTGCTGGGCAACCTGAGCCGGGTGCTCCGACTGGAGGAGTCCTTTGCTCTCCAGTTGCGCCGCTTCTACTTCGACGAGCGGTTTCGCAACGAGCCCGTCGTGGGTATCTATCTGTTCCACCAGCCAGCCCTGCTTATCCGCGATCTCCAGCTGGTCCGCACCGTTCTGGTTGAGGATTTCGTCAGCTTCTCCAACCGATTCGCCAAGTGCGACGGGCGCAGCGATAAAATGGGCGCCTTATCGCTATTTTTGGCCAAACAACCCGAATGGCGCGAGATCCGCACCCGTTTAGCACCAGCCTTCGCTGGCGCCAAGCTGAAACAGATGTTCTCGCTGATGGAGGAGGTGAGTCACTCAACCAGCCGGGTCCAAGTTGCAAACTCCCGCTCCCAGGTCCACGACACAC	ACTCTTACACCGACCAACACAACACACATTTCCACGCTTAATCGCGGGGCCCGATTGAAGAAAAAAATAACCAACAATACTATTTTACTCTGGTTTGACTTGTAATTACACCGATTCCATTTAAAGATAGGAACTGCCTGTTATTCAAATCGAATTCCATTCCCTTACAATCGAAGTGTTTCGCCGGCATATCGTTTTGGATCATAGCTATATTTTAATATTTCGAGGTGCGGCACTTACACCAAGCTACTGGGTAAAATTATTAAATTCAATTTTTTAGGTTCCATTAAATTTCCTCTTCTGATTGTAATGTTTCCAATTTTGATCAATGAGTTACGAAATGGCATATCTTATATTATTGATTATTAATGATAAAAGAACCTCTCTAACGGGCCATGTTGCTATCTGGGGCTTATCAAATCGAACCTGTTTATTGTTACTGACTTGTTGTATCTTATCAAAGGTCACCACCAACTACGGCATTACTACGAAAAAAAAACCATAACGTTTATAGCTCAAGACTTTAGCTCTCCTCTGCTTAGTTTGTTGCCTAGTCGAACCAAAAGTTCTATGCAAATTAATGCTAAGAAATCACAAACTGTGTGCGTTTCAGTAAAACAAATCGAATTTACTAATCATTGTGATAATGCAAATTCCCATGACAAAGCTGAAACTCATGTCCAAACGGTTGACAAGTCTCTAAGGTTTTTCTGTTGTATATCTGTTATGCAAATATCAAGAATCACATCGAGGCACTAATGAAACAAACACTTAATGAAACACTGATTAGCTCTAGCAGCAACATTTGGATACCTATTTATTGATTAACAATCCGTTTGCTCTGTGCTCCTTGTGGGATGTTCTATATCTCCGTAGATCGGCTGCGACTTGGAGTGGTATCTGAAGCGTCTGACTCGAGATCTTAGACGCGGCGACGCGGAGCGAGGGGCGATTGTGAGCATCAAGGACGTGTGTGATCTGTACAACACCGACATGATAGCCAGCATCGCATTCGGACTGAGATCGTACAGCCTGAGGAACACGCAATCGGAGATCGGCTCACA

Amplicon 5'

Amplicon 3'

ACTCCACGAACATCCTGCTGGCGATAGTGACGATCCTGACGGGCGTGTTCATCTGGTCCCGGCGCACCTACGTCTACTGGCAGCGCCGGCGGGTGAAGTTCGTCCAGCCGACCCACCTGCTGGGCAACCTGAGCCGGGTGCTCCGACTGGAGGAGTCCTTTGCTCTCCAGTTGCGCCGCTTCTACTTCGACGAGCGGTTTCGCAACGAGCCCGTCGTGGGTATCTATCTGTTCCACCAGCCAGCCCTGCTTATCCGCGATCTCCAGCTGGTCCGCACCGTTCTGGTTGAGGATTTCGTCAGCTTCTCCAACCGATTCGCCAAGTGCGACGGGCGCAGCGATAAAATGGGCGCCTTATCGCTATTTTTGGCCAAACAACCCGAATGGCGCGAGATCCGCACCCGTTTAGCACCAGCCTTCGCTGGCGCCAAGCTGAAACAGATGTTCTCGCTGATGGAGGAGGTGAGTCACTCAACCAGCCGGGTCCAAGTTGCAAACTCCCGCTCCCAGGTCCACGACACAC

ACTCTTACACCGACCAACACAACACACATTTCCACGCTTAATCGCGGGGCCCGATTGAAGAAAAAAATAACCAACAATACTATTTTACTCTGGTTTGACTTGTAATTACACCGATTCCATTTAAAGATAGGAACTGCCTGTTATTCAAATCGAATTCCATTCCCTTACAATCGAAGTGTTTCGCCGGCATATCGTTTTGGATCATAGCTATATTTTAATATTTCGAGGTGCGGCACTTACACCAAGCTACTGGGTAAAATTATTAAATTCAATTTTTTAGGTTCCATTAAATTTCCTCTTCTGATTGTAATGTTTCCAATTTTGATCAATGAGTTACGAAATGGCATATCTTATATTATTGATTATTAATGATAAAAGAACCTCTCTAACGGGCCATGTTGCTATCTGGGGCTTATCAAATCGAACCTGTTTATTGTTACTGACTTGTTGTATCTTATCAAAGGTCACCACCAACTACGGCATTACTACGAAAAAAAAACCATAACGTTTATAGCTCAAGACTTTAGCTCTCCTCTGCTTAGTTTGTTGCCTAGTCGAACCAAAAGTTCTATGCAAATTAATGCTAAGAAATCACAAACTGTGTGCGTTTCAGTAAAACAAATCGAATTTACTAATCATTGTGATAATGCAAATTCCCATGACAAAGCTGAAACTCATGTCCAAACGGTTGACAAGTCTCTAAGGTTTTTCTGTTGTATATCTGTTATGCAAATATCAAGAATCACATCGAGGCACTAATGAAACAAACACTTAATGAAACACTGATTAGCTCTAGCAGCAACATTTGGATACCTATTTATTGATTAACAATCCGTTTGCTCTGTGCTCCTTGTGGGATGTTCTATATCTCCGTAGATCGGCTGCGACTTGGAGTGGTATCTGAAGCGTCTGACTCGAGATCTTAGACGCGGCGACGCGGAGCGAGGGGCGATTGTGAGCATCAAGGACGTGTGTGATCTGTACAACACCGACATGATAGCCAGCATCGCATTCGGACTGAGATCGTACAGCCTGAGGAACACGCAATCGGAGATCGGCTCACA

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