• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00581

Gene CG11406

CG11406

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2R:24206105..24206123	60C2	FBti0199419	78957

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CGCATCGCTGGCATGGAAACTATATG	GCGCGGCAACTTCCTAACCCTTATTT

Images

Construct Design Sequences

sgRNA sequence	GCTACACTAGCAAAAAAGAGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGCTCGCGATTTATTTGCCA	TGACAGTTGAAAGCTGTAGGA	CTAGTGTAGCTTAGAACTAAG	GTTCTACGCAGAGATAGTGG

 

Amplicon 5'

Amplicon 3'

CGCTCGCGATTTATTTGCCATATTTCACGGCAAAACAAAATTTAAATGTACCGTGTGCGCGGGAAACAAAAAGTGTTCAAGGCACCGTGGCGTATACGTAATGTGTGTTATACTCTAGTTAGCCGCCAGCTGGAGCGGCGTTTTCGGTGGGGCAGAGGGGGGAAAGTTTGGAGCACCTTTCGGTTGCACATTATGGTGGGCATTTCGGATTCTCCAGAGTCTATGCGGAAATACTCTAAATATTAAAATTTAACATACGGTTAGGAAATAAGCGATCTATTTTTATAGGCACTTATTTGGTTAATCACATAAAGAACCTACTGGTGTGCAGTTGCTAAACTAATTTAAAGGTATTTTGATATATTTCGTGTATTCAAATAATCTGGATACACAAACTTGACTCGTACGCACGCACCAATTAACCCGCTCAGTTGGGCATTTATCACCCCTTCGTAATCCCCTTTTATCCGCATCCACAATAGTTGTGGACCGGCTGGCCACCAGGAAGCTCTGCCCGGCAGGAGCAGCTCGTTTACGCATTTAAATTCAAATAACTTTTATTACCGAAACTAAGGCCTCCGCCCTTCCTCATTCGGCCCTCGATAGTACGTATGTTTGTCTGTTTCCGGGAGTTTCAGGGAAGTGGCCTTGAGGGGTGGGGGGATGGTAGGTGAGAGGCGGGAGGTGGTCCCACTCCAGAGTGCAATAAAAACTTTTGCATACAAGAGGAAAAAAGTTTCGGGCGCTTTGTTGGCTAAATTATGGCCCGCATATATAATCGTACTCACACGATCCAATGGATAGTCGCTGAAGCCCACATAGACGGAGGTGAAGAGGGTGCAGAGGGCGTAGGTGGGCCGGGAGGCGGTGCAGAAGAACTGGCCGCCCACCTGGGTCAGCGCCGACGCCGGGAGAAGTTCGTGGAGGCCAAGTTTGTTCAGCAGCAGCTGCACGGAGAAAATGCCACCAGTTTTAGTTTCAGTTGAACTTTGAGCTATGTTATTTGCAATTAAAATCAGAATCATGCTTATCCTTTAAATCTAAATGTAATATGTCCTATTTTCCTACAGCTTTCAACTGTCA

CTAGTGTAGCTTAGAACTAAGACAAATGCTAATCTTCTCTAGCAAACTGCGTACTTCCCGAGTGACAAGAGCTTATCACGCGCGCAGCACGCGTCAAGTATACGCACCGTGGCCATGGAGCTGTCGCTGGCCAGCAGCCGCAGTGGCGGACTGCTCAGGTGCTGGAGGAATGCCACCGGAGCCAACAGGGCGGCATTGGCGAACCGCGCATTGTACTCCGGCCGCTGCGAGAGCAGCACCAGGAGAACGGTGGTGCCCTGCGAGTGCCCGACGTAGTGCAGCTGGCGGCGATTCGTCCTCGCCAGCACGTAATCCACGATGGCGGGCAGGTCGTAGATGCCAATCTCGTGCCAGCTGAATCGCCAGAAGCGGGCGTCACTTGTCTGGAGCGTGCGGTGCCCGCGGGAATGGGTGGTGCCCCTGGCGTTCGCCAGCCAGACGTCAAAGCAGCGGGCATGAAGCTCCAGCGCCAGGGATCTGCCCCTTCCGCCCGACACGAAGTCCCCGGCGGATCCGAGGAGGCCGTGCATCAGGAGGAAGGGGCGCAGCTGTTGGGGGCACCAACGGTTTTGGGGAGCGGGAATGCGGTGCAGGCTCAGCCGGTAGCCATCGGCGGTCTCTATGCGGTGCACTTGGCACTGGAGCTGTTGGCGCTGCACCGCCTGGCACACTGAGTCTGACTAAACCAGGACAGTGGACCACTATCACCACCACCACCACTATCTCTGCGTAGAAC

 

---

Search Again