CR00581

CRIMIC CR00581

Gene CG11406

CG11406

Information

Donor Location Cytology FBti Stock number
pM37-p02R:24206105..2420612360C2FBti019941978957

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
OK OK CGCATCGCTGGCATGGAAACTATATG GCGCGGCAACTTCCTAACCCTTATTT

Images

Construct Design Sequences

sgRNA sequence GCTACACTAGCAAAAAAGAGTGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
CGCTCGCGATTTATTTGCCA TGACAGTTGAAAGCTGTAGGA CTAGTGTAGCTTAGAACTAAG GTTCTACGCAGAGATAGTGG
 
Amplicon 5' Amplicon 3'
CGCTCGCGATTTATTTGCCATATTTCACGGCAAAACAAAATTTAAATGTACCGTGTGCGCGGGAAACAAAAAGTGTTCAAGGCACCGTGGCGTATACGTAATGTGTGTTATACTCTAGTTAGCCGCCAGCTGGAGCGGCGTTTTCGGTGGGGCAGAGGGGGGAAAGTTTGGAGCACCTTTCGGTTGCACATTATGGTGGGCATTTCGGATTCTCCAGAGTCTATGCGGAAATACTCTAAATATTAAAATTTAACATACGGTTAGGAAATAAGCGATCTATTTTTATAGGCACTTATTTGGTTAATCACATAAAGAACCTACTGGTGTGCAGTTGCTAAACTAATTTAAAGGTATTTTGATATATTTCGTGTATTCAAATAATCTGGATACACAAACTTGACTCGTACGCACGCACCAATTAACCCGCTCAGTTGGGCATTTATCACCCCTTCGTAATCCCCTTTTATCCGCATCCACAATAGTTGTGGACCGGCTGGCCACCAGGAAGCTCTGCCCGGCAGGAGCAGCTCGTTTACGCATTTAAATTCAAATAACTTTTATTACCGAAACTAAGGCCTCCGCCCTTCCTCATTCGGCCCTCGATAGTACGTATGTTTGTCTGTTTCCGGGAGTTTCAGGGAAGTGGCCTTGAGGGGTGGGGGGATGGTAGGTGAGAGGCGGGAGGTGGTCCCACTCCAGAGTGCAATAAAAACTTTTGCATACAAGAGGAAAAAAGTTTCGGGCGCTTTGTTGGCTAAATTATGGCCCGCATATATAATCGTACTCACACGATCCAATGGATAGTCGCTGAAGCCCACATAGACGGAGGTGAAGAGGGTGCAGAGGGCGTAGGTGGGCCGGGAGGCGGTGCAGAAGAACTGGCCGCCCACCTGGGTCAGCGCCGACGCCGGGAGAAGTTCGTGGAGGCCAAGTTTGTTCAGCAGCAGCTGCACGGAGAAAATGCCACCAGTTTTAGTTTCAGTTGAACTTTGAGCTATGTTATTTGCAATTAAAATCAGAATCATGCTTATCCTTTAAATCTAAATGTAATATGTCCTATTTTCCTACAGCTTTCAACTGTCA CTAGTGTAGCTTAGAACTAAGACAAATGCTAATCTTCTCTAGCAAACTGCGTACTTCCCGAGTGACAAGAGCTTATCACGCGCGCAGCACGCGTCAAGTATACGCACCGTGGCCATGGAGCTGTCGCTGGCCAGCAGCCGCAGTGGCGGACTGCTCAGGTGCTGGAGGAATGCCACCGGAGCCAACAGGGCGGCATTGGCGAACCGCGCATTGTACTCCGGCCGCTGCGAGAGCAGCACCAGGAGAACGGTGGTGCCCTGCGAGTGCCCGACGTAGTGCAGCTGGCGGCGATTCGTCCTCGCCAGCACGTAATCCACGATGGCGGGCAGGTCGTAGATGCCAATCTCGTGCCAGCTGAATCGCCAGAAGCGGGCGTCACTTGTCTGGAGCGTGCGGTGCCCGCGGGAATGGGTGGTGCCCCTGGCGTTCGCCAGCCAGACGTCAAAGCAGCGGGCATGAAGCTCCAGCGCCAGGGATCTGCCCCTTCCGCCCGACACGAAGTCCCCGGCGGATCCGAGGAGGCCGTGCATCAGGAGGAAGGGGCGCAGCTGTTGGGGGCACCAACGGTTTTGGGGAGCGGGAATGCGGTGCAGGCTCAGCCGGTAGCCATCGGCGGTCTCTATGCGGTGCACTTGGCACTGGAGCTGTTGGCGCTGCACCGCCTGGCACACTGAGTCTGACTAAACCAGGACAGTGGACCACTATCACCACCACCACCACTATCTCTGCGTAGAAC
 

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