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CRIMIC CR00582

Gene AnxB10

CG9579

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:20173285..20173299	19C1	FBti0199420	78958

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCGCTGTACTCCTTTGAGCAGTACAT	TTCTTCGAGATCCACTTACCACCACG

Images

Construct Design Sequences

sgRNA sequence	TAGCGAGTGACGCATCGCCGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGTACTCCGGTCACGATCAG	TCGCTACACGGGAAGAAAAA	GTGGCGAATCAATAACAGGCA	GTCGCCAAGAAATCAGGGGA

 

Amplicon 5'

Amplicon 3'

CGTACTCCGGTCACGATCAGCGTGAGCAGGCGGCGGAAAAAGCCGGAGGTCTCGCTGCACATCTGCTCGGCCAGCGGGCGCTGGTAGCGCTCCTCGTAGACGGCCACGATCTGGGCCATCTCCTCGTTGGTCTTGGTGCACAGGATCTCGACGAGCGTGGCCTCCTCGGTTCCGATGCCCGCCATGGCGGCGTGCAGTTGCTTGCACAGGTACTCCACTGGTGGCATCATTAGACCCACGATCACGTCCTCGAACTTGCCTCCCAGCTCGTCCTTAAGATCGTCCACCAGGTCGCGCTCGAACTCCGCTTCGTAAACCGCCTTGATCGTCTGCCTCTGCTGGTTGCTCCTGCCGACGAGCACGTCGATGATTTCCTGCTCGTCGGTGCCGAATCCCTTCATCGCCGCCCGCAGCACCTGGGCGTCCTGGGAGGCGTCGAAGGGAGCTGCGTCCTTAACCGTGGGCACGGGCTGCACAGTAAAAATTTAGTTGTATATTAAATATAATTTTAGCAAAAAAAAAAAAGCCTAATAGTTTAGCAGCCTAACAAATATTATGGTCATATTCAAATCATTCATTTACACATATAACATTTCAAATATCAGAAAGTAGCTGCATACAATGAGGATTGAACTTCCTATGGTAACTCTCTGTTTTCGATAGAAACTAATATTTTGGAAAGTTAATACATTCAAACTCAAATCATTAAGCCATATGCATTTTTCTTCCCGTGTAGCGA

GTGGCGAATCAATAACAGGCATTAGTGCAATAATGGAATCATCTCTAGCCTGGCAATCCAATCCACTCACTTTGTATTCCATTATGCAGCTGGAAGATTCCTTGGGTTCCTTGTGGCTCTTCAGTAGACGACTTCACACGAATCAGTCTTTCTCCTGGGACTTTTTTGCGAATTCTTCTTGCGACGTCTTGTCGCCTCTGCCTGCTTTTGCTAAATAATTCGGATTGGGAGGCACTGCACCGAGGTCTCTTTTCAGTGCTCTCCGGTTTGCGCTCGGTGCAGAGCACAAGTTTTACACACGAAGAAAAATGAGACTTGTTTTTGGATGGCAGAATCTGAAGGCAGCACTCGGCTACGTAAATTAAATACACATATTGTATTGCTTTTGGCTCAAAATCGATCGGTTTTTATCTAATTTCTTTTGTTTTAACATAGAAGGAATATTTTTGAATTCTTTTCGAATTTGAAATCGAAAGTTTTTTTTTAATTTTAAAGCATTTGTTATCTGTTTTAGATAATGGATGCGATGTATTGCAACATAGGTACCTGCCATTTCCCCGTTTGAAGCCAATCTTGTTTCTCTCAGTGTATAAGCTTACGCGAGCGCGAGAGAGAGCTTTTAATTCCGCTTCAATTGGGGTTGCACTTGAATTCCAAATCAACTGATTCATTCGGCACATTTCTTGAATTAATTAATTAAAACCTGGCGATGAGATCGTAAAAGCTTAAAATGTGCACAAGCATCTAGGTTTTAATGGAATTTTTAATGCGCTTTATGCCGAAAGAGTCGAAAATGGATTCAAGTGTCTTGGAAAAAGTCGCTTCAAATGTTTTCAGATCTTAAAAAACAAAATATCGAATAAAATCATTTCTGAGGGTATACACTTTATATCCGAGCCAAATAATAGACATCTTTTTCTCGTAGGATATCTTGTATAGATATTATGTTTATTTAATCTTGTTAAATAGGCTTCGCTATAATACATTTACATTTGGGCTCTACTAAAACAAACAATGGATTACCAATCAAATATGCGGGTAGTTCTATTCTTATGTATATATATATGTACATATGTATGTATGTTTGTCTATATTCTGTCAAGTAGAGAATGTTGCAGTTTCCCCTGATTTCTTGGCGAC

 

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