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CRIMIC CR00583

Gene CG1812

CG1812

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:20512173..20512179	19D2	FBti0200594	80599

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	TATCACCACTGGACGAAACTTTGGCC	CGGAATGGCGGTCAACTCATACCAAT

Images

Construct Design Sequences

sgRNA sequence	CAGAGATCGTGCCACATCTGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGCTCCGCAATCGATAGCTA	TGGCACGATCTCTGAAGCAG	GTGGGATCAGCTGAGCTACG	GGTAGGCCCGTTCGGATAAG

 

Amplicon 5'

Amplicon 3'

CGCTCCGCAATCGATAGCTAAATGCGCATCACTAGCGGTGCTAGCCGACTTGGCAGTTCAAACGTCAAGAGAAGAAACGTCAAAAACGCGAAAAGTAAAAAGTCGCAAGAGGGAAAAGAAGCAAAGAGGTAGCAACAAGCAAAGAAATCGCACGCGAAACTTTTTTCTCTGCCAGTGTTTAGCAATAATTTAGCTAATTTCGTGCGTATATTTGTCGTAGAACGGTTCATAGTTCGCCAAATAACGGTTTGTAGACAGCTGGCCGCGAGTGTAAACAGAACGTGCTGCCCTCTCGCTCGCACTCGCACTCGCGCACCAGAAGCTCGGTCATCTCAAGAATTTCTTATCAGACGGATTCCCCAACCTTGGGACCACATAGGTTAACATATATATAGGTTAATAGCCCGCTCTAATCTTTGGTCCGTTCCGAGAGAGTACAGCGAACAGCATGGCAGCGACGCCAGCGCCAGCAGTGACGCCGGCAGCGACAGCAACGTCGGCTGCGGCCAAAATTCCTGTGAATCCCGTGTCCCCCGAGGAGGAGACCCTTCTGCGGGGCCTCAACAAGCTGCGCAACGAGGAGAAACTCACGGACGTCACTCTGATCGTCGAGGAGCACCGATTCAAGGTAGGACCCCACCCACACCATTATAGATCCGAGCAATAGCTACATGGAACAGCCCCATCTGTGGATCTGCTTCAGAGATCGTGCCA

GTGGGATCAGCTGAGCTACGAGCGGTTACGTCTAGCCTCATGGACACGGCACATCGGCGAAGGCGGATGAAGTTGGGTTGCACAAGTTTGGCTCCCAGAGTGCGCATAGCAAGTATGCATAGATTATCTTTCAGAGCAACTTGGTTGTGATTAGGCTAGGTTAGTTACGACAATACCTTCAATGGCCTGCGCATAGGACAAATAGAAATCGCACTAAACCGGATTGCTCGCATCCTAAACTCGGCGGTTTGGCAGTTTGGCTAGTTGGCCAGTTGGCCATTTGGCCATTTTGCGAGCTCGTGTTAATTATATATCGGAAACCACGAAAGCATAAGGTGACTAGTTATAGGGTTAGAGTGGCAAGTGGTAGGCGAATCGATAATCGTGGCCAGCAAAAAAGCTCATCTTTACTTTCTCTTGCTAATAATACAAATACGAAAACAAATTCGCGACACAAATTGATGTGTTTCGGCCGATGACGCATTTTCATCGCGCTACCTATGAATATTTAGGGATTCCCGGAATCAGACTCATATATTCAGATACATACATAAATCCAGAAAATTCATAGAAATGTTTTATGATACAGATTTTCAGAAATCTGATTTCAAATGTTTTCAAAAATAACATGGGGAATATCAGCGTTTTTCGTTACACTTATTACACTTATAGTAACATTTTTCCCCAAGTAATCGAACGAAATACGTAATCCCAACTTTGCAGGCTCACCGCGTCGTCCTGGCAGCCAGCAGTGACTACTTTTGCGCCATGTTCGCCGACTGTGCCATGATTGAGTCGCGTAAGGACGAGATAAATCTCTACGGTATTAAAGCCAGGGCCATGGGCTCGATCATTGACTACATATACACATCGATGCTGGAACTGAACTACGATAACATCGAGGAGATCCTGGCGGCAGCCACGCACGTCCAGGTGCGCGAGGTGATTGACCGATGCACGATCTTTCTGGGCGCAAAGATCGAGATGGAAAACTGCCTGGCCATTGCGGGAATGGCCGATATCTATGGAATTATGGACTTATCCGAACGGGCCTACC

 

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