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CRIMIC CR00584

Gene CG8668

CG8668

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:8132415..8132419	28E7	FBti0199421	78959

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATGGCGGCTATGGCCAATCTTCACAA	GTGAACACGTCCTCCAGCTTCAGATA

Images

Construct Design Sequences

sgRNA sequence	ACGAAACCTGGTCAAATAAGCGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AGAGTGGGTGCGCTTAAGAC	TTCATTCTCCATTGTCGTGGA	TGCAGTCCGCTTATTTGACC	AAGTTCTCCTGGGTAAGCGC

 

Amplicon 5'

Amplicon 3'

AGAGTGGGTGCGCTTAAGACTTAGACGCTAATTAAGCTAATAATGTTTATTCCACAGGCAACATCAACAGTAGCACCAACAACAACAGCTCTGCCGACACGTTGGCTGTTAAACAATCGATTCCCGCCCAGTCACCACAACAACAACTGCCCCAGTCACAGTCGCAGGTGCAGCCAGGCCTTTCCGATGCCGACCTCCTCATACCCACTTCCAATTTGCAAAAGTTTATTGAGAACGCCGATAGGATACTGAAGAACATCACTTCGAACAGTAGCACGGGTAATTATTATACTATTACTTAGGGGGCGCTAGACCTTTGAGACCTATTAATCCAGGTTTCAAATTGTTAAACCAGATCGATAAACAGTTTAGTTGCTCCTTCAAATTGATCCTCTTTCTGTTTCATCTTGCGTAATAATTTGCCTTAGTTTTGTTTTGCTTAGACAAAGACCCGCTGGCGGCGCACCCATTTGTATAACTCCACGACAATGGAGAATGAA

TGCAGTCCGCTTATTTGACCAGGTTTCGTGTTTCATTATGAAAGAGCTGTAAACATGACATATTGTTTCATACTATTGGATTACTGTCGAACAGATGTCTATGGGGACGGACTAGAATTCGCTTGAGTGTTTGTTTGCATTTCAATTAATGGTGTGGTTTATCTTTCTAAATAAAGGCAATCCATCGGATATGGCACAACTCAACGCAGTGGACGGTAAACTGGAAGTCTCGGGCGTTGAGTCCGAAAAGCAGGAACCGGAATCCAAGGTGATACAGGAAATTAAGGAGGCCAAGAAAGAGGCGGCAGTCCCTCTGCCAAAATCCGCAAAACCACCAGTGCCACCTGTGAAAACGACGAAGAGCAAGGTGCCAAGTGCTCCTGTAGATCCCTCCAAGGGAGTGGCCACCGAGACACTCTACGAGCCAGGCCACGTGGATGAGGAGATCGATGCGGAGCGCATATGTCCGAAAGGCGGAGAGTTTATTAAGCTCCTGGTTCTTATTAGCTCAGCGATGTCTCACGATGCAGCCCGCATGTCCATCCGGCAGACTTGGATGCATTACGGGACGAGAAGGGACGTGGGCATGGCATTTGTCCTAGGACGTGGCACCAATGAAACCATAAACAAAGCGCTTACCCAGGAGAACTT

 

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