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CRIMIC CR00591

Gene Oatp74D

CG7571

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3L:17509920..17509923	74D1	FBti0200599	80602

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
short band	OK	ATTTCATCTTCGGCGAGCAGCTGATG	TTGGAAATCCTGCAGCTTGTGCAAGG

Images

Construct Design Sequences

sgRNA sequence	TTGCATACTTATGAGGGCAGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTGCACTCGCTGGTATGTCA	TGGTTCATGTTGGTTGGATCTC	ACCACTGCCCTCATAAGTATGC	GCCGACTGTATCACACCCAT

 

Amplicon 5'

Amplicon 3'

CTGCACTCGCTGGTATGTCAACTTCTCGAATCCCGGCTTCGACGCCACGGATCCGCGCTGGATTGGCGCCTGGTGGCTGGGGCCTGTGGCCATTGGCAGCCTCATGCTGCTGGCCTCCATCGCCATGTTCTCGTTTCCCAAGCAGTTGAGAGGCAAGCAGAAGCCGCCGGGGCAGACAGCAACTCCAGCAGCTCCAGTTGAGCCGGAGGAGAAGCCCAAGCTAAAAGGTGAGAATGTCAAAGATAGCTTGGTCTTTAGTATTTGATGATAAAGATTTTAGATTATAATCATGCCTCTGTCATCAACAGATCAGCCAATCAATGCACCTTACTCCATCTGGAACTAAATCTTAAAATTTACCCATACAGATTTTCCCAAGACAGTCCGTCGCCAGCTGAGCAACGACATCCTGATGTTCCGCACCGCCTCGTGCGTGTTCCACCTGCTGCCCATCGCCGGTCTCTATACGTTCCTGCCCAAGTATCTGGAGACGCAGTTCCGGCTGGCCACCTATGATGCCAACATGATCGCCGCCTTCTGTGGCATCCTGGTCATGGGCATAGGTATTGTCATTTCCGGGCTCTTCATCCTGAAGCGAAAGCCCACTGCCAGGGGCGTGGCCGCCTGGATCGCCTTTACAGCCCTCGTCTACTCGGCGGGCATGATCATCCTGATGTTCATCGGCTGCAGCATGAACGACTTTGCCGGCTACAAGCCAAGCGATGGCAACAGGTGGGTCATCGAAGAAGTTCTTGCTTAATTACATATTAGTTATAATTATATAATATAAGTAAACTATAAAGATAGCATTTGAAAAGGTAGAGATCCAACCAACATGAACCA

ACCACTGCCCTCATAAGTATGCAAACTTTTTCCGCAACAATAGTATCCTTTTAATTTTTGGATACATGTAGAATTAATATATTGCACTAAGTGGGACTGCGGGAAAGTGGGGCTATAAGCTAGCTATCAAATCCAAAATGTAATTCGTGTTAAGTCGACTTTTTACACTTGATATACGGAGTAACTCTAGTAACATGTAACTTATTTTAATCCATCGCTAGTCCCGCCTTGATCGAGCCCACGTGCAGTGCCGCTCTCAACTGTACCTGTGATAAGGAGAACTTCGCGCCCATCTGCGCCGACGGCAAAATGTACATCTCGGCCTGCCACGCCGGATGCAGCAGCTCCTCACTGCGGCCCAGCGACAATCGCACACTCTACTCCGATTGTGCTTGCATTCCAGATGGTGAGATGCACCCATTGCCATAGCCCTAAGATTCAGTATCTTAATCCCTTTGCACCTTTCAGCTCCGGAGGCGGTCAACGGTTACTGTGATAATAACTGCAAGAACTTCATCTACTTTATACTGATCTTTGCCATTTGCGTATTTATGCATTCCACCTCCGAGGTGGGCAGCATGCTGCTCGTCATGCGCTGCACACACCCCAAAGGTAATAACCAGGATTGGCAAACAGATGTTACACTAACGATAACAAAACCCTTAATTCCCCGCAGATAAGGCCATGGCCATGGGTGTGATACAGTCGGC

 

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