• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00593

Gene Hexo2

CG1787

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	X:8710234..8710239	8A2	FBti0200600	80603

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	GTGTATCTTTGGAGGGATAAGACGGTG	GAAGATCTCCTTGAGCACTGCGTACA

Images

Construct Design Sequences

sgRNA sequence	GCCAACGGATAACAAATGGTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTATGTGGGCCACTCTCCAG	TTTGTTATCCGTTGGCAGTTTT	TGGTGTCTATGCTCAGCAGG	AGTTACGGGCCGTATCCAGA

 

Amplicon 5'

Amplicon 3'

GTATGTGGGCCACTCTCCAGGGGGTGGGGTTTGCTTCAAAGAGTGGGAGATATGCTGATTTCACAGCACTACACAGGCAGAAAAGACGTGGCACTTCATGCATCAAGTGTCCTGTTAGTATAGGGAAGCTCATTTAATAAAGCTGTAAAATCTAATTGCCAAGCATTAATGTGGAGTAGCAGTGTAATGCAAGGACTTGGCGTTTCTCGCAGTGTAATTTCAATCCGCGCTCTCTCGCGCTCTTGCACTCTCTTCAATTCAATTGACGATAAGCTCGACTTGTTATTGCTGTTGTTGCGGCCGCTACTGCGCTGCAAGTGAAATTTCGCTGGATTTATTTTATTCGCTTTTTCGATTTCAGCTCTTCAGTGTTGTTGTGCTCGGCGAGCGGAGCGGGTGGGCGGCCGGTTGAAAATAGCGAATACACCGTACACACCCCCCTAACACAACAAATATAGCAAATACCGAACAGAAATTATAAAAGCGAGCGGCGCAGCCAAAGAAGCCGTTAGAAAGAAACGATGCGGTTCAGCGGCTACAATCGTTATCAGTGCTTTTGCTCTGCTGTTGGATCGCTGCTGCTATTATCCCTATTATCCTTCGCGGTCGGCGCTGCGCTGACGCGGGCAGATGACGTCGGCAGCGACGCCGACGCCGGCAGCGCCAGCAGGTTTGTGTCTTTGCTTCATAAAGGAAATCAGTGCAACAAGTGCAAGTTTTTTTTTAAATACATGTTTTGTTTTGGAAGTATAACAATTTTTGCTAACAAAATGCAGTGAAAATACAAAAAACTGACTGTTCTTTATTTTCAATGAAATTTCAAGTGTTATATGCGCTCAGCAGACTAGTGAAAAAAAGTCCAAAAAGTCCACTACTTTTAAATATATTAAAGGAAATCTATTCATTCTACAAAAGAGAACTTTAAAAAACTGCCAACGGATAACAAA

TGGTGTCTATGCTCAGCAGGATATCCAATATCTCCTCAAATAACTGAGAGCAACAAGTGAAATCAGTCTTAAAAACTAGAAAATAAAACAAACAGAAGAAAACAGGTGAAAATTTAACTGTGCCAAATGCATTTACATGTTAATTTGCATTCGCTCCAAGTGCAATTTTCGTAAAGGGAACAAGTGCAAAAGGCAAAGCGACAGAATAAGAACTACTTTCTATGCAGCTGCATATATTATTGAAAAGAATTTGCAATAAATAAAGTGAAATCTGCTTTATGTATCTAAATATATATGTACTTAGCTTTATTATAGCAATATCTTCCGAATTTCCAGGAAATGGCTGTGCAGCCGGACGGATATCTGCACCGCGGAGGGCGAGATGGTGGCCGGACTGCAGTACGCGCCGGAGATCTTCGAGAGCCAGCGGGATTGCCGACTGTCGTGCGGCAAGTACGGAGCCATCTGGCCCATGCCCACCGGCAAGGAGTGCACCATATCGCACAGGCGGGTACGATTCGATCCGTGGAAGGTGCGTTTCCATGTTGTGGCGCCCGGCGAGGCGGCCACCCAGTTTCTCAGGGAGACGAACCGGCTGTTCGTCTCGAATCTGCTGAAGGAGTGCATACGAAACTGCACGCTGGAGACCAGCAAGCAGATTCTGGTCAGATCCACGGTGGCCAACGAGAGCCTCGTTCTGGACTGGCCCACCGACGAGAGCTACGCCCTGGTGGTGCGAACCACTGAAACGGCCACCTTTGTGGACATCCAAGCGACGACGGTGTACGGTGCACGTCATGCCTTCGAGACGCTGAGCAACCTGGTCACCGGCAGCCTGTCCAATGGCCTGCTGATGGTCACCACGGCCAACATCACCGATCGTCCGGCCTTTTCGCATCGCGGCGTCCTTCTGGATACGGCCCGTAACT

 

---

Search Again