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CRIMIC CR00594

Gene fbp

CG31692

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2L:19762353..19762366	38A2	FBti0199423	78961

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCGATGTATCAGATCTGCCGGCAGAA	AGTAAGCACCTAGCACTCCGTCTGAT

Images

Construct Design Sequences

sgRNA sequence	TAAATGTGGCTAGAAGATATAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CTAAGTGCCTCCTCCACGTC	CACATTTAATGACGTTTCCCTAT	AGGGTTAGATCTTAGAGATAGAGCC	ACCCCGCTTCAGTTGTACAG

 

Amplicon 5'

Amplicon 3'

CTAAGTGCCTCCTCCACGTCGGACTTGGATCCTAGGAATATGGGACTGCGCTCGTGGATCTTCTTGGGCACAATGTCCAAAATGCTGATCTTTCCGTCGCTGGCCAGACCTCCAGCCTGGATCATCAGATAGGCCATGGGCACGCACTCGTACAGCAGACGAAGTTTTCCGCTGGGAGCGGACTTTGTTGCCGGATAGATAAAGATGCCGCCGTATTTAATGGTGCGATGCACATCCGCGACCATGGAACCCACGTACCGCGCTCCATAGGGCTTTCCCTTGGCGGGATCCTTCTTGGCCGCAATGTAGTTGAAGACACCATCCTCCCAATCCGCTGCATATCCCTCGTTGATAGAGTATATCTTTCCCTTCTCCGGCACCCGCATGTTGGGATCGGTCAGCACGAACTCTCCGATGGCCGGGTCATAAGTGAAGCCATTCACTCCCGAACCCAGACCCAGGACAATTGCTGTGGCCGAACCGTATAGCGCGTAGCCGGCGGCCACCAGCTGATTTCCGGGCTGCAGTGCATCCTCCACTGTGGGCGGACCATCGCTTTTCTTGCGGTAAATGGCGAAGATTGAACCGATCGACACCAGGCAGTCTATGTTGGAGGATCCATCCAAGGGATCGAAGCACACGATGTATTTGCCCTGGAGGTGGAAGAGCGAATAAATTATAGGGAAACGTCATTAAATGTG

AGGGTTAGATCTTAGAGATAGAGCCATCTTAACTGACAGCTAAAAGCTAATTCACACCCTCATACCAAATTTCGTAACAATTGGTCACGTATTAAGGCTTAAAGACTCTAATATATCCCTACCTGTTTCTCCACTTCCACCTCGATCACATTCTCGTTCTCCTCGGAAACCATTAGACATGTGGTATAGGATGACTTCAGCATGTTGATGAACAGCTCGTTGGAGAGCACGTCCAGTTTCTTGACCTCCTCGCCTTGGACATTCACGTCGCCAGCGAATCCATGGAGCTTGGCGATACCTGCCTTCCGCACCGCGGATGATGTAGCCTTGATGGCGGTCTGGATGGAGTTGAGCAGCTGGGAGAGATCGCCAGTGGCGCTCTTGAACTTTCGCTGCTCCTGCAGCACGAAACGCGTCAGCGTCATCGCATTGGAGTCGAAAGCTGGCCTCTGTTGGGTCATTTTGGCTATTTTATATGCAAAAAAATTTAGTTGTACAGTATAATTCGCTTAGCTGCCTCGAGTACTTTGCACAATGCCTCGATGCAGGTAACTTAAAAATGCAGCTAACTTAATTTTTTTTTTTTCTATTTTCTATTTTCTATTTACACAAAAAAATGGAAAAATAATGCAGATATCTTGCCGATGCAGCTAACTATCGATGCAGCTAAGCGAATTATACTGTAGTATAAAACAAGATTTTAAATTTTACTGGATATGATTGGGAGAGGATTAGCATAGGATTGGAATAAATCGATTAAATATTAGTAATATTACTTTGTAAGAAAATAAAGACAACGAGCTAAGTAATACATAACATATATAGGCAGTGGAAAGGATTTAGGATGGATTAAATGTACAACTGCTGTACAACTGAAGCGGGGT

 

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