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CRIMIC CR00595

Gene nudE

CG8104

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	3L:9900367..9900369	67D1	FBti0199424	78962

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GCCGGCCAACAAATCGATAACGTTCA	GCCTTAAAGTTCCGAGCGCATTGTCA

Images

Construct Design Sequences

sgRNA sequence	AGACTCAGTTTGCGTATATGTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTCCATGCAGTGCGTTGTTG			AGTTTGCGGAGGGACTTACG

 

Amplicon 5'

Amplicon 3'

TTCCATGCAGTGCGTTGTTGTATGGGAAACTTCGTCGTAGCTATTTGTTGTTGTTGTTGTTGCTGCTGCTCATCACCATCATCCTCGTTGTTTTCGTTGTTGTTGCCGCAGCAGCTGCGTCACAAAAATGAGTTTTGTTTAATTTGATTTTATTTACATTTGATATGGACAAAACCGACTGCCCTTTTAGGGCATCGAACAAGAACAACAGCAACAGTAACTACAAAATTCAGGGCAAACACCTGTTCCTAATTGATTTTTCACTTTTCTTCGCCGACTTCTAGTCTATTCACCACCCCCAACACACAAAACAAAAAAAAAGTAAAACTACGACTTGAACAACAACTGTGTTTTGGCATTTTAATCACAGCAATTAAAAACAAAGCAGCCAAAAAACACGAATAAATAACCCAAACAGTAATAGACCAACCGTAGTACATAGCATTGTTCAGAAATTAAAAGTTAAATCAATTCGAACGAACGAAATTACAAACCCATTTTGACCGCCGATTAGTACACAAAAACCAGAAAAAGGAGTTAGCAGCTAAGGAAAATTTGCAGAAATGGAGTCGCCCCCGATGTTCAATAGTGTGGAAGATGAGTGCAGATATTGGAAGGAACGATCTAAGCAGTACCACAAAGAGTAAGTGCCCATCCCTATTACAGTCTCCGAAATCAGCCATATAGCCTAGTTTTACTTTTAATTTTCAGGTGGACAGACGTGAAACAGGAGTACGACGAGTTTGTGGAGCAGTCCCGCGAAATGGAAATAGAAATGGACGCCACTCTGGACCAGAAGCAGAGTATAATCAAAGATCTCACAGCCAAACTCACAATGTTTGAGCGGGAAAACGAGTCTCTCAAGGTAGCTATAAGCTAAGCCCGATTTACACAAACTGAGCTTAATTCTTAGACTCAGTTTGCGT

TATGTGGCTTTGAGCTCCCGACAAGATTAGTCTTTACAACTATTTCATTCTCAGCTCAAGTTGGAATCGCATGGCATCGATATGTCAAACATGGAGAAACAGCTGGAAACGGTGAAGAAGGATCGCGACACCATGAAGGTGTACCTGCGGCAGCTGGAGCAGAAAAACGACGATTTGGAACGTGCCCATCGCATACTTAACGAAAGCATAGAGAATTTCGAGAAGATGCTGGATCAGGCCTACGAGAAGAACGCCCTTCTGGAGCTGGAAGTGGATGAAAAGGGATTGCTGCAGGAAAAACTACAGCGATTAATGGATGAGACGAGAGGTGGATGCATCTATACCAGATGTCCAGCACAGACTTTAAAACTAATTCATCAAATCTATTGCAGATCTCAAACAAGAGCTGAACGTCAAGTCACGCTTTACCCCAGTGGTCAATGGAACGAGCGTTCCCACGGCAAACGACACAAATACGGTCAACAGCTCGATGAACTCGTCCGCCTCGCTGCCAAATGGTATTGTTGCCAATGGCGAATTGGTAAAACACGACAATGCTGTCGCCACAAGAGCCACCAGCGTCAGCGTCAACGCCCTCAATGGCAGTTTAGTTAATAGAAACGAATATAACCAGCAGCATTCGCTTAAGAGTAAGATACTAAGTTCCCTAAAGAAAACCAGACGCGCGCTAAAACTGTCCAAGACGAATCGCCAAACGTTCAAGCCATGCATTTAGTATTGTTTTTCCATTTGTTTCTTTTTTACTTGTTAACCAAAAAATGTAGCCCGCTTAAGTTCCTCATAATTTGTAATTGTATTGTAAATTATTAAAATTAAGAGCCCAAATGTGACGAAAGAGTTTGCAACATTTCTTGTAGGCGATTTTTGTACGATTTGTGTTTGCGTCTGTAGCTAGTAGTTGACCTAACTAACCTCTCGACTAACATGCTGTGTACGTGTGCTTCCTCTTGCAGATCCCGAGAATCAAATCAATGGCAATTCCATGAACCCCAGCTCCCGCACAACGGCGCTCAACATTGTGGCCGACATGTTAAGAAAACTAAACGTAAGTCCCTCCGCAAACT

 

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