CR00599

CRIMIC CR00599

Gene klhl10

CG12423

Information

Donor Location Cytology FBti Stock number
pM37-p02:71003..71015HetFBti0200601Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L HA-R Forward Reverse
no band short band CCTGCCGTTGGACTTATGGGATGAAT GAAAGGACCTACCAACCCAGCGAATA

Images

Construct Design Sequences

sgRNA sequence GAGAGCATACTCAGTTATGATGG
sgRNA2 sequence
 
Amplicon PCR 5' Left Amplicon PCR 5' Right Amplicon PCR 3' Left Amplicon PCR 3' Right
TTCTGGCGGATCCTTCTTCG GCACTAACACTATTCCAGCCG AGTATGCTCTCTTCTACGTCGC AACGGGATTAAGCAAACGCG
 
Amplicon 5' Amplicon 3'
TTCTGGCGGATCCTTCTTCGACAGTAGACCTTTTAGACCTTTCTTTAGGTAGACCTTTTTATTTTATTAATGTCGCGGTCACTACCGAATCCAATTAGAACGACATTGCAGCTTTCCAGCTTCGGCTTGCCAATATCACAGAGATCTTCCCGTATGACATTGAACTTACTGCCCGTATCGAATAACCGTACACTTTTTATTTGCAACATAAACTTCTTTGCTGGTAAAATCATTTTTAGAAACCAAACAACAGTCATTTGTATTTAAAACATTTTTGTTTATTTTTGCGCACTCTTTAGAAATATGGGCATATTGATTTGTAACACTTTCTGCCTTTTTCTTTATTCTGACAATCATTCAGCACATGACCTCTTTCACCATAGTTGTAACATTTTACCTTTTTTTTCGCGTCTTTGATGACAATCGGATTTTTACTGACTCTTTCTTTCTCACTGTTCTCGTTGCGCTTTAACTTGTCTTTCTCACTCGGATTTTCCTTTGCAATTATTGTTCCCAAACACTTTAGTTGGTCTTTAAATTGACTCATATTTTTGGCACCATAAGTCGATAAGAAGTCATAAGTCCATAAGAAGTCAGTCGAATACATGAGCGTCGCCAGTTCTTCGTATTCTTCTATCCATACTTCGGCTGGAATAGTGTTAGTGC AGTATGCTCTCTTCTACGTCGCGAAAATTGATCGCATTCTATCATTTTCTGTAACTACGTTGCGTCTACTCCTTGCACATTTTCATCACTTCCTTCAGCATTCGTTTTCCCGCTCGGCAACCCAAACATAACGTTTTATGCTTCACCGTTGGTTTCATTGTCTGTTTTATCGTCTGTTTCGACATCGTCAACTTCGGTTGACGCCAGTAAACTGCTCATCTTCCAATTGCGAAAAATGTGTACAAGTAAGTCTTCTTTTTTGTACCCTATGCCTGAACGTTGCATATTGAAACAGCCTGCGTCAAATTATTAACTGCGTATTCCCTCTTTGCACTATATCTCTGGTCGTTTTCGTCAAAATCGAAACCAATAAATTCCCGTAATCTTTGACGATTACTTCAGTCTCCTTCATTCTCGAACAACAAACTTATGCAGTGCAGCAATTCTCTCTTTCTTGCTTGTTCGTAAGCTAGCACTTATAAAATTATTTAAACACGCCATTGCAGTAAAAAATTTCGACTGTATTATTTTTTGTTACACCTTCAACTTTCATTTAATGTCATCAATTTTGTTCACATATGTTCAGTACCGTTCACATTTGTTCAATTCCGTTCGCGTTTGCTTAATCCCGTT
 
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