CR00599

CRIMIC CR00599

Gene klhl10

CG12423

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	2:71003..71015	Het	FBti0200601	Order from Bellen Lab

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	short band	CCTGCCGTTGGACTTATGGGATGAAT	GAAAGGACCTACCAACCCAGCGAATA

Images

Construct Design Sequences

sgRNA sequence	GAGAGCATACTCAGTTATGATGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTCTGGCGGATCCTTCTTCG	GCACTAACACTATTCCAGCCG	AGTATGCTCTCTTCTACGTCGC	AACGGGATTAAGCAAACGCG

Amplicon 5'	Amplicon 3'
TTCTGGCGGATCCTTCTTCGACAGTAGACCTTTTAGACCTTTCTTTAGGTAGACCTTTTTATTTTATTAATGTCGCGGTCACTACCGAATCCAATTAGAACGACATTGCAGCTTTCCAGCTTCGGCTTGCCAATATCACAGAGATCTTCCCGTATGACATTGAACTTACTGCCCGTATCGAATAACCGTACACTTTTTATTTGCAACATAAACTTCTTTGCTGGTAAAATCATTTTTAGAAACCAAACAACAGTCATTTGTATTTAAAACATTTTTGTTTATTTTTGCGCACTCTTTAGAAATATGGGCATATTGATTTGTAACACTTTCTGCCTTTTTCTTTATTCTGACAATCATTCAGCACATGACCTCTTTCACCATAGTTGTAACATTTTACCTTTTTTTTCGCGTCTTTGATGACAATCGGATTTTTACTGACTCTTTCTTTCTCACTGTTCTCGTTGCGCTTTAACTTGTCTTTCTCACTCGGATTTTCCTTTGCAATTATTGTTCCCAAACACTTTAGTTGGTCTTTAAATTGACTCATATTTTTGGCACCATAAGTCGATAAGAAGTCATAAGTCCATAAGAAGTCAGTCGAATACATGAGCGTCGCCAGTTCTTCGTATTCTTCTATCCATACTTCGGCTGGAATAGTGTTAGTGC	AGTATGCTCTCTTCTACGTCGCGAAAATTGATCGCATTCTATCATTTTCTGTAACTACGTTGCGTCTACTCCTTGCACATTTTCATCACTTCCTTCAGCATTCGTTTTCCCGCTCGGCAACCCAAACATAACGTTTTATGCTTCACCGTTGGTTTCATTGTCTGTTTTATCGTCTGTTTCGACATCGTCAACTTCGGTTGACGCCAGTAAACTGCTCATCTTCCAATTGCGAAAAATGTGTACAAGTAAGTCTTCTTTTTTGTACCCTATGCCTGAACGTTGCATATTGAAACAGCCTGCGTCAAATTATTAACTGCGTATTCCCTCTTTGCACTATATCTCTGGTCGTTTTCGTCAAAATCGAAACCAATAAATTCCCGTAATCTTTGACGATTACTTCAGTCTCCTTCATTCTCGAACAACAAACTTATGCAGTGCAGCAATTCTCTCTTTCTTGCTTGTTCGTAAGCTAGCACTTATAAAATTATTTAAACACGCCATTGCAGTAAAAAATTTCGACTGTATTATTTTTTGTTACACCTTCAACTTTCATTTAATGTCATCAATTTTGTTCACATATGTTCAGTACCGTTCACATTTGTTCAATTCCGTTCGCGTTTGCTTAATCCCGTT

Amplicon 5'

Amplicon 3'

TTCTGGCGGATCCTTCTTCGACAGTAGACCTTTTAGACCTTTCTTTAGGTAGACCTTTTTATTTTATTAATGTCGCGGTCACTACCGAATCCAATTAGAACGACATTGCAGCTTTCCAGCTTCGGCTTGCCAATATCACAGAGATCTTCCCGTATGACATTGAACTTACTGCCCGTATCGAATAACCGTACACTTTTTATTTGCAACATAAACTTCTTTGCTGGTAAAATCATTTTTAGAAACCAAACAACAGTCATTTGTATTTAAAACATTTTTGTTTATTTTTGCGCACTCTTTAGAAATATGGGCATATTGATTTGTAACACTTTCTGCCTTTTTCTTTATTCTGACAATCATTCAGCACATGACCTCTTTCACCATAGTTGTAACATTTTACCTTTTTTTTCGCGTCTTTGATGACAATCGGATTTTTACTGACTCTTTCTTTCTCACTGTTCTCGTTGCGCTTTAACTTGTCTTTCTCACTCGGATTTTCCTTTGCAATTATTGTTCCCAAACACTTTAGTTGGTCTTTAAATTGACTCATATTTTTGGCACCATAAGTCGATAAGAAGTCATAAGTCCATAAGAAGTCAGTCGAATACATGAGCGTCGCCAGTTCTTCGTATTCTTCTATCCATACTTCGGCTGGAATAGTGTTAGTGC

AGTATGCTCTCTTCTACGTCGCGAAAATTGATCGCATTCTATCATTTTCTGTAACTACGTTGCGTCTACTCCTTGCACATTTTCATCACTTCCTTCAGCATTCGTTTTCCCGCTCGGCAACCCAAACATAACGTTTTATGCTTCACCGTTGGTTTCATTGTCTGTTTTATCGTCTGTTTCGACATCGTCAACTTCGGTTGACGCCAGTAAACTGCTCATCTTCCAATTGCGAAAAATGTGTACAAGTAAGTCTTCTTTTTTGTACCCTATGCCTGAACGTTGCATATTGAAACAGCCTGCGTCAAATTATTAACTGCGTATTCCCTCTTTGCACTATATCTCTGGTCGTTTTCGTCAAAATCGAAACCAATAAATTCCCGTAATCTTTGACGATTACTTCAGTCTCCTTCATTCTCGAACAACAAACTTATGCAGTGCAGCAATTCTCTCTTTCTTGCTTGTTCGTAAGCTAGCACTTATAAAATTATTTAAACACGCCATTGCAGTAAAAAATTTCGACTGTATTATTTTTTGTTACACCTTCAACTTTCATTTAATGTCATCAATTTTGTTCACATATGTTCAGTACCGTTCACATTTGTTCAATTCCGTTCGCGTTTGCTTAATCCCGTT

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