CR00600

CRIMIC CR00600

Gene CG34325

CG34325

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p0	X:16720100..16720106	15A3	FBti0200602	80604

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CGCTCTGCAAACGTTTCGCGTGTTTT	TGGCGTCAAGTGCTCTCGATTGTTGT

Images

Construct Design Sequences

sgRNA sequence	CACGACTTGGAACGCCCACTTGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
CGAAATATCGTGCGGCACAC	GGCGTTCCAAGTCGTGTTTT	GGCAGACTTGGTAACTCTTGAAA	TTCCAACTAACTGGTCGCCC

Amplicon 5'	Amplicon 3'
CGAAATATCGTGCGGCACACAGTGCGTATACGTGATGGCCATTATCCCATTTAAACTGTGAGTGAAATGCCGAAATAATGGCTTTGTAATATACGGCAAATTGTATATACATACGATTATCTGCCGGGAAACTTTTGCGGAAGATCGATGGAGCGGATTATTCAAAGACTTATGCATGGCGAAAAACATTGGAAATTTTACTATTCGGAAGGCTGCAAATTTATGACAACATCTTTTAAATAAGTTAAGATAAAAAGGAAGCTAGGCATAAATTTAGACAATTGCTAGAAAGTTTAATTTATTTCAAGATGAACAAAAAAAGCAACTAATCGCAACATAAAGAGACACTAAAATATGTTATAAATTACATAGAGGAAATTGCAAATCAAAGGCATTTAAATAACATAAACAAATAGAAACGATAATGGAAAGTTTTTGTATAAAACATATCTCAAATGAAAACAATATGCATATTTATAATAACAAGTGAGTTGTTGAAAATCTATAAAAGAAGACCCAGTTTCTATAGAAACCTCAAGTAGGCCAATCGAGTTGTTAAGAAATATAAAAGCAAATCGTTTTCATTCATCATTTGCAGCAACTATTAGAAGATCACAAAACCAAAGTCAAATGACTGGTGTGCTTAGAAAAGTGACAAGCCCAAAAAGTAACATTAATTTCCTTGTGTTTTCAGTGGTGGTTAGTCGGGTGAATAAACCAATTTCATATATCAGTCGTCGAGACACTGACCAGACTCCATCACTCCCTCCCCGATTCCCAATCCCAATCCCAATCTCCAGCCCTGTCTCTGTCCCTCTCCAGATCACGATCGTTATGGTGATGGTGATGGTGGAACATAACGATGATCGCGGTGATGACTCTTCAAAACCTTTTGGTTAAGCAGGCTTCGCCAAAGTGGCTGATTTATATGGCTCATTTTATTAAGCGGACAACGAATAAATCGCTTTGTAAATTGATGATATTTTGTGTGTAAAAAGCAGGGGGATGATATCATCCGAGGCGAGATGTTCTGGGCTTAGCTTAACCTTAGGACGCTAACTATATATTATAATGTATATGGCAAGCGGGGCCCTTAATCCGCACTTTTGCTATCGCCCACTGATAAAAAACACTATTCGAAACTCAAATATTTTCCAAAACACGACTTGGAACGCC	GGCAGACTTGGTAACTCTTGAAAATCACTACTATTTCATTATTGTTTTTTTTCTTTTTTTTTCATTTTTCAGTTTTAGTATGGTAATTAACATAAAGTCTAATAAGGCAAGGCAAACCATATTCGAGTGTGCGTGAATTTTTATTTTTGTTTGTTTTTTTTTTCTCGCTGATTTTTGTTTTTTCTTTTTCTTTGGTAGCCAAAAGCCAGATGGATGACAATTTCAGTTGGGTTTAGTTGATTTCGCTGGCAGTTGACACTCTCAAAATTCCGCTCACACATTTGTGCCATGTACATGGAAAGCTGACAACTGAAAACTAAAAACTTTCGGGCCGAAAATCGAAAGCGGAAAACTTAGCACACACACACAACACACAGCACACACCGAATGGGTGGCCAACGCGGCGTATGAGCAATCTGCAACCTGTTTGAAAAACGCCCACGCCGGAGATTGGGGATTCGGATTTGAATTGGCTTTGGGTTTTGGGTATTGGGCCCAGCCAGCTGGGCGACCAGTTAGTTGGAA

Amplicon 5'

Amplicon 3'

CGAAATATCGTGCGGCACACAGTGCGTATACGTGATGGCCATTATCCCATTTAAACTGTGAGTGAAATGCCGAAATAATGGCTTTGTAATATACGGCAAATTGTATATACATACGATTATCTGCCGGGAAACTTTTGCGGAAGATCGATGGAGCGGATTATTCAAAGACTTATGCATGGCGAAAAACATTGGAAATTTTACTATTCGGAAGGCTGCAAATTTATGACAACATCTTTTAAATAAGTTAAGATAAAAAGGAAGCTAGGCATAAATTTAGACAATTGCTAGAAAGTTTAATTTATTTCAAGATGAACAAAAAAAGCAACTAATCGCAACATAAAGAGACACTAAAATATGTTATAAATTACATAGAGGAAATTGCAAATCAAAGGCATTTAAATAACATAAACAAATAGAAACGATAATGGAAAGTTTTTGTATAAAACATATCTCAAATGAAAACAATATGCATATTTATAATAACAAGTGAGTTGTTGAAAATCTATAAAAGAAGACCCAGTTTCTATAGAAACCTCAAGTAGGCCAATCGAGTTGTTAAGAAATATAAAAGCAAATCGTTTTCATTCATCATTTGCAGCAACTATTAGAAGATCACAAAACCAAAGTCAAATGACTGGTGTGCTTAGAAAAGTGACAAGCCCAAAAAGTAACATTAATTTCCTTGTGTTTTCAGTGGTGGTTAGTCGGGTGAATAAACCAATTTCATATATCAGTCGTCGAGACACTGACCAGACTCCATCACTCCCTCCCCGATTCCCAATCCCAATCCCAATCTCCAGCCCTGTCTCTGTCCCTCTCCAGATCACGATCGTTATGGTGATGGTGATGGTGGAACATAACGATGATCGCGGTGATGACTCTTCAAAACCTTTTGGTTAAGCAGGCTTCGCCAAAGTGGCTGATTTATATGGCTCATTTTATTAAGCGGACAACGAATAAATCGCTTTGTAAATTGATGATATTTTGTGTGTAAAAAGCAGGGGGATGATATCATCCGAGGCGAGATGTTCTGGGCTTAGCTTAACCTTAGGACGCTAACTATATATTATAATGTATATGGCAAGCGGGGCCCTTAATCCGCACTTTTGCTATCGCCCACTGATAAAAAACACTATTCGAAACTCAAATATTTTCCAAAACACGACTTGGAACGCC

GGCAGACTTGGTAACTCTTGAAAATCACTACTATTTCATTATTGTTTTTTTTCTTTTTTTTTCATTTTTCAGTTTTAGTATGGTAATTAACATAAAGTCTAATAAGGCAAGGCAAACCATATTCGAGTGTGCGTGAATTTTTATTTTTGTTTGTTTTTTTTTTCTCGCTGATTTTTGTTTTTTCTTTTTCTTTGGTAGCCAAAAGCCAGATGGATGACAATTTCAGTTGGGTTTAGTTGATTTCGCTGGCAGTTGACACTCTCAAAATTCCGCTCACACATTTGTGCCATGTACATGGAAAGCTGACAACTGAAAACTAAAAACTTTCGGGCCGAAAATCGAAAGCGGAAAACTTAGCACACACACACAACACACAGCACACACCGAATGGGTGGCCAACGCGGCGTATGAGCAATCTGCAACCTGTTTGAAAAACGCCCACGCCGGAGATTGGGGATTCGGATTTGAATTGGCTTTGGGTTTTGGGTATTGGGCCCAGCCAGCTGGGCGACCAGTTAGTTGGAA

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