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CRIMIC CR00604

Gene daw

CG16987

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2L:2811041..2811041	23B2	FBti0200604	80605

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	TTTGATGATCTGTCGCCGCTGGACTT	GCAGAGCTTACCAAACTGTAAAGTGCC

Images

Construct Design Sequences

sgRNA sequence	CTTATCTGGTAACTGACCCCAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATGACACTCTCCCATCCGGA	ACTCACAACTGTGCTGGGTT	TGAGTTCCTGGGGTCAGTTAC	TCAACTAGTGGCAGCTCAGG

 

Amplicon 5'

Amplicon 3'

ATGACACTCTCCCATCCGGACGATAGCACCAAGAACAAGGAGCTGGACGATTACTACGCTCGCACTAGCAAGAAGTTCATACTGCTCAATAGAGGTAAGCAAATCTAGATTTAAGCACTAGAAAGTAAAGATTGAAGCTTAACTTCAAAGGGAATCCTTTTACCATTCTGATTTTGCTAACACAACCCTTTATCCTTTATTTTTACAGAGGAGGTCGAGTGCAACCGAGCGAGAGATGGAAAATCCAATCCGTCCATGTGCTTCACCTTCAAGATAGACGACGCAGATGCCGAGGGCTTCGACGTGAGCACCGCCGTCCTGTGGCTCTTCAAGAACAAACAGAACCGCACCGACACGGCATCGGTGAACAGCACCAGTGCCCAGCAGACGATCGTAGTCTCCGAGGTGGAGGTGGATCAGCAGAAGGACTCCAAGTATTTGTCGGCGGCCAAGACCATAGCCATCCAGTCGGTGAATGTGCAAGGTGGGTACACATAGTATACATTAGTATAGCATAAACACTCATCCCCCCAGAGCGTAATCATGCCCAGAAAAGAATCGCACACGTTTGGACCTTTGGTCGGCAGCCGCGGAACGGCCAAAACTGACCCTCAATACCCTCGGGGGACAAATCAAAATATGTGACATGTCTATACTAGATGGGTATTATAAAAATTATAAAGAACGCCGATAAGTGCCAACGTTGACAACCGCTTGTTTGTGTTTTCAATTAGACAGATCATTGCGCAGATTCGTGATAGCACTGCTTGATAGCTCGGCTCGAATCTGAAATTCATGTGGGTGAATAACTTGACAAAGCATTATTCGTGGGTGCAAAAACTGAACTTTGGCCACAACCCAGCACAGTTGTGAGT

TGAGTTCCTGGGGTCAGTTACCAGATAAGAAAGATGGGATCTACGATTCTGGGAGAAGAGAAGATGGCTCTGTGGAACCCCCAACTAACCGCTCTGCCTTTCCTTTCGCATACTTTCAGATGAGTGGATGAAGATCGACATTGAGTGGCCCATCAAACACTGGATCAGTGGCCACGAGCTGAGCCACCTCATCCAAATCACCTGCGGTGGCTGCGATGTCAGCGACATGGAGGAGATCATTTCGGTGGACAAGGATTACCGGCCATTCATCGTGATCGACATGCAGAACAGGCGGCGCAAGAGTCGCCAGAAGCGCAGCATCAACTGCTCCAGCGGAATGACCGAGTGCTGTCGGGAGCACCTGTACATATCATTCCGGGACATCGGCTGGAGCAACTGGATCCTGAAGCCGGAGGGCTACAATGCCTACTTCTGTCGCGGATCCTGTAGCTCGGTGGCGAGTGTTACGCAAGCGGCCTCCCACCACAGTTCCATAATGGTAGGTACCCCATTCGACAGGAACTGATGTGTTGATGATGAAACTAATGTATATCCTCTTCCCTTGCAGAAAATTCTCTCCACGAGCGGTGCTAACAAGTCGCTGGAGTTGGTGCCCTGCTGCACCGCCAAACAGTATTCCTCGCTGCAGCTGGTCGTGATGGACTCGAGCAACACGGCGACGGTGAAGACGCTGCCCAACATGGTGGTGGAGTCATGTGGCTGCAGATAGAGAGGCGAATAGAATTAGCCAAGTTACATTTACGTTACATCGGACAAATTAGACGTAGACTCCATTTCGCTCCATTTCAGCTAGCATCCAACCATCCAACTACATTTCTCTCCAACTAATTTAAACTCATTTTGCAAAGCATTTACATTAGCGTTGTCATTTAATCGTAGTTACCTGTAGTGCCTGAGCTGCCACTAGTTGA

 

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