• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00608

Gene CG6472

CG6472

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	2R:16882597..16882607	53E1	FBti0199427	78965

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCGCTAGAATCATAAAGCCGGTACCC	GACCAATGCTCACCAATGATTCCCAG

Images

Construct Design Sequences

sgRNA sequence	CGCCAAGTTCATGGCCAAGTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AAACACCCACGCTTCGATGA	CCATGAACTTGGCGGGAGTA	TCTTCGTTAGGCCAGTTTCGA	CTGGATCGAGGGCCGTTATC

 

Amplicon 5'

Amplicon 3'

AAACACCCACGCTTCGATGATAATATACAAGTTTGGCTTACAGCATAATTTATTAAATTTAGGAATTAATTATCAGCACTTGTTCAACAATTAGAAACTCAGGGGGAGAAGACCATTCCAAAGTTGATATGGTTTTTTATTGAGCAAAACGGAAGGTGATAAGTGGACGGAATCGCAAATGAGTCCGGCAGTGAGTAAGGTCAACGTCATTGTTAGTTGGCATCTAATCAAGTGTATTATCCGTATGCATTCGCAGCTGACGATAAAAATGTGTAGCAAAACAGAGAGTACGAAAAAGCCGTCATTGACATCCCAATGTGGTTGACCCACTGACGGTGGTGTTGCCTCTTAAAGACACCACCCACTCGCGAATCGATCTGATTAAGCACTTGGCCGTGTGAACATCGAAAACCTGATGGAAATGAAAACTTGTTTTCATTTTGCCACCGCTGCTACGTAACACGCGGCGTATGCGTCACGTGCTGCCTAGTGGCTACTGCCACTTGTGTTTTTGTTGGCCCCACAATACATGCCATTCCACTCGATTTCATTTACTCCCGCCAAGTTCATGG

TCTTCGTTAGGCCAGTTTCGATTTCTTTGCTACTGTTTTTGTTTGACTTTTGGTTTTCATCTTTGTTATTACTTTTTTTTGGATTGTTATGTTATGGCTGCGGCTACCTGGTTGTTGGACGCGTGGGTTAATGAAGCTAATGAATCAATTAGTTTTGTATTCCAAGCTCTCAACAAATTGGCTTGCGCTTACCACTTGACAAATTCGATTTCCGCCTTCATTTTGTGGCTACGTTGTAAGAAGTTGCAGAACAATTTGCTTACAAACATTTTGTTAATTGGTTTCCTTTCAGTAAGTCTTTTGACAAAATAACTTCTTATTTCTTTTATAATTTCCTATTTGGATTGTATTTTTTTTGTATATCCCAGCTGGTTCACCGATTTTCTACAGCGCCGCTCCGCGTGGATCCTGCTCCACTTGCTGTGCCATTAAAGAGCGCGAGGACATAAAGTTCATGCTGTACACCAGGTAAGCAAGCGGCGACCCATCTTCATTTCATCCCAGCTCATGCATATTGAGGGGCCATCAAAAATGGGTCCACCTTTTGCAGTCGGAACCGCAATTCCGCACAACTGCTCCACCTGAGCGACGATGCCCGGCTGGCGCAGAGCAATTTCAATTTCAATTACCCGCTGGCCATCTATCTGCACGGCTTCTCGGAATCGGCCACCGGCGAACGGCAGAGCAGCCAGGAGCTAAAGGACGGTGAGTGGGGACTTCCTGCCACCTCGACCAAAAAAATCAACAATATATACCCTGTCCATCTTCCATCCCTGTCCGATTTCCCCCGGCAGCGTTTCTCAGGAGGGGCAACTACAATGTCATCCTCATCGACTGGAGCGCGATGACAGCGGTGCCCTGGTACTCGAATGCCGTCGAGAATCTGCCGGTGTCGGGTCGCTATTTGGCCAGGTTCCTGCGATTCCTGGTGGACAAGGGTTACCCAGCGAAGTACATCCACTTGATTGGCTTCAGTCTGGGCGCCGAGGTGGCCGGTTTTGCGGGCAAGCAGCTGCAGGAGTGGGGCATCAAGCTGCCCAGGATAACGGCCCTCGATCCAG

 

---

Search Again