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CRIMIC CR00616

Gene CAH8

CG5379

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p1	3R:22388159..22388174	94A11	FBti0199429	78967

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	TCTCAAGAGTCTGCAGTCTGCTTACC	CAGTACTGTAACGCCATCGCTGTACT

Images

Construct Design Sequences

sgRNA sequence	AGGCTACGCAATAGGTGAATAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ACCTCCTGTTACGTAGGGCT	GTAGCCTTTGCCCGTACGG	GGTTGGTTCCGGGTCGAAAA	CCAAAGAGCCGCGTATCTGA

 

Amplicon 5'

Amplicon 3'

ACCTCCTGTTACGTAGGGCTTCACGCCATAGATCGTCGTCGGAACCTTAAATTCCACTATCAACGGAAATGGATTAGTTTTCCCTTTCACTTGGAAGTCCGTCTTATAAAAACCTGTGTGTCCACTATTCCTAATTGTCACCAGCTCATCGAAGAACTCATCATAAAGTGCGAAAATCAGAGGCGGCAAGTTAAGGGGTATCGCCTTGCGTCTGCTAATCGCAATGGGCGACTGTTCTGATCCACCACAATTTGGGTATTTTTCCGGCCACCTCTCCGGGGATTTGTAGTCGAAATCAGTCGATCTGAAATCTGTAACAAAGTTATATAAGTCTTTACAATTCCCACACATTCTTGATTTATCTTGGGCGAGCTTCTCCATATTTGACGAAATACCCAAGAGCATCCACCACCAATGGACGTGGTCACTTTGCAAAACCATTAGGAAGGCCGTTGGAGCAATTTCTGCACTTGCCAATTTCCCAAAACGTCTTCGCAATTTCAGCTATTTCGCAGAGCCTACGGATTATGTCTAAGCGTAGCATAATTGCTTGATTTTGGGTCCGGACCATGGAAACCTCCTTTTGTTTACATTGTTCCGAGCGATGGATCGCACACGCCGCTCTTATAACAATGGATGGTACTTCGACATCTACTCCGCCCCCGAAATTGACGCGCTCATTGGGTTTCATGGGGCATTTGTGGATGCAAATTTAATTATTGTTATTATTTACAGCCACCGGGTTGTTTGCTATCCATTAGATGAACCGCGATGAGACAGCTATATGGGCGTCGAGTGTGACATATGAGGGTTCGGTTCGGTTCGGTTCGGATTGGTTTAGTTCGGTTCAGCCGGAGGGGATGGCTCATAAATTCCCGTACGGGCAAAGGCTAC

GGTTGGTTCCGGGTCGAAAAATCTCAAGATCTGGGGCACGTTGTCTGGGGACGACGATCAGTCTGCTATCGATGGCGTCTCAATACCAAGGTGATCCACAGTTGTATCGTATTTCGTTGGATGCGACACACCTCACTGGCTTGCATTTCGGTACTCACTTGCGGCGAACGGCAGCAGAAACAGAAGACGCTGATAGAAGTCATCAAGAGCCGGAAGATGCATTTTCCACCTGATCACTCGCTATACACTACTACAGGCTATAGGAGTTGTCTTCGCCAATCTAGTCCAAATACGCTCCACACAGTAACCCCTGACCGAAGACCGAAGATTTCCAAGCGCGCGTAGCTCTGCAGACTGAACCACGATCGGCTTAGGCCAAAACCTTTATACCAAAGGGCCACAGTCTGGGCTTTGGTTTGGCTTCCGTCTCCGTCTCGTGGACTGGGAGAGCCACATTGTCAAACGCTCCACGGAGCAGCAATAGATGGTAATCTCGACTCGAAGTTGTCATACCCGCTACGCGTACTGCTGTCTGAGCACCGTTACCCCGAAATTAAAAACTATAATTGAAAAACTTGGTTCGATGGGGACAAATGCAGATTACACAAATTGAAGTTGAAATTCCTTGGCATAACTTAACCCGTCTATGGTATAATATCTAGGTTCTGCAAGTTCCATTTATAAGGCGTTTTATTTATATAACGGAGAAAGGAAAAGTTTATTCATATATTTAGGTTCCAAATAAAGTACTTCATACTTTAACATTGTTTAATAACTTAATATTGCGTATATAGGTAAGTTTGGACATATTTGAAAGTTTAAAATACCCTGCATCTTTTTTGGTTACAATATTTTAAAATATTTCAAATGTTTTTTTTTTTATTAAAACGCAAGTAAATCGTGTTTGCTTTGATCTCTGCTTATGACATCCTCTTGTAAGTCAGATACGCGGCTCTTTGG

 

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