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CRIMIC CR00621

Gene JhI-21

CG12317

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:12053504..12053513	33B11	FBti0200607	80607

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	AAGGTGTGCGTCATAAGCTTAAGGCG	GTATTGAAGATGGCCCGGCAAATCTC

Images

Construct Design Sequences

sgRNA sequence	AGAATACCACCCAGTGGGTGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TTAATGGGTCGGGGCAAGTC	ATGCTGCCTCATCCCTAACG	CACTGGGTGGTATTCTAGGAGG	TTAATGGGCGGCTACCTTCG

 

Amplicon 5'

Amplicon 3'

TTAATGGGTCGGGGCAAGTCGGGCCTGAAAGGAATACACATTACACATTGGGTTCAGAAGAAGTCAGTGATATCACTCACCTCTTATGCCGGAGCCACAACATTCCGGCTATGCTGGCCACCACTGAGAGCCAGAGCACCGAGCTGAAGTAGTTGATCAACTGGTACACGTTATCCGTGAGCAGCATGAGCAGCGACATAAGACACTGTGGGAAAAATGCGCTTACATCAATGCACAATTCTCTGCTGATAGCTGATTGGTGCAAAATGCTCATGCCAACTCACCGTGAAAATCAGGGAGGGAATGGGCGTCTGTTGTTTCACGTGGAACAGCTGGAAAAACTTGGGCAAATGCCCCTCCTGAGCCCCAGTAGCGAAAAGGCGCGCCGATGTGAAGAGCACCCCGTTGACGCCACCAAATGTGCTTAGCGCCACGAAAATGGGCACCATGAAGGCCAGCGGTCCGAACACTCGGTTTCCAAAGGTCACCGCCACGGCCAGCGAACTGAGCATCTCCGGCTTGTTTACCACGGCGAAATAGGCCAAGTTGACCAGAACATAGATGCTCGTGACCAGGGGCATGGCTATCCAAATGGCGCGCGGCAGATTCCTGCGCAATGAGAAAACAAAGAAACCGAGATCAGTTTTATACAAAACAAAAACTTCTAACTACTGTTTTTGCAAATAACTACTATCTTTAATAGTTGACTATGATGTTTCATTCGGAACTAAAACATTAAAAAGCTTTCAGGTAAAATAAGAGATATCTTATCACTTTCGATGACCGTTGATATAGAAAACATTTACCTTATACTCGAAAAAATCATAATTGGTTTGTAATAACTAGAAACATCAGAACTAAAACTAATCTACGGGCTTTACTGCGCATAAGAATTGAGTTTCCTACTTGAAATTTGTTTCAGTGTGTCAAAGAACGTGCTATTCAGCGAGAAAGCCACTTGGCTGTGCAGGGAGCGTAGCACTTAAATTGATTGCAAATTGAATTCGTTAGGGATGAGGCAGCAT

CACTGGGTGGTATTCTAGGAGGAATGACATCAAGGACACTCAACAAGTTTCCACTTGTTGCTGCCTGCGCATGACGCACAAGGAATGAATGAGGCAAATAAACCCAGCCCAACCCAACCATTTATCCTTGTCACCACTACTCACTTGTATGGATCCTGTAGCTCCTCCGTCACAAAGTTTAAGTAGTTCCAGCCACCAAAGGCAAACAGACCGGAGTAGAAGGCGTAGCCAATGTTGCGGGCCGTGTAGATACCCTCCCACGGATTTCTGAAGTTCTCCAGCTCGCCAGTGGCCATGTAGTACAGTCCGGACAGGATAATCATGATGAGGGCCAGCAGCTTGCCCACCGTGAACACATCCTGCACTTTCATGGACACCTTCACCGACAGGCAATTGATTGTGGTGAGCAGAGCTGGGAGAGTGGGAGTTTCAATTAATTAACATTGAGTGGATTGTTTAACCAACTTACTTAGACATATGGCAGCCAGCAGTTTCACCGCATTCTGTGGGGGATCGCATTCCGGAAAGAATGGCTTGACCGCATAATGGGCAAAAGATAGAGCCACAATGGTCTAAAAAAAAAAATAAAACAATGTATTCATACTATAAATCCCACATGCCATTTGTTTCTAGCGGAGAAAGCTGTCCTACTCGTAATTGAATTCAATTCCGGTCGAAGGTAGCCGCCCATTAA

 

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