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CRIMIC CR00622

Gene ppk

CG3478

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:14380406..14380430	35B1	FBti0199605	79278

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	AGAGTATCCCACTGGATTCCAGGAAC	CCAGGAAACTCAGACATGAGATTCCC

Images

Construct Design Sequences

sgRNA sequence	CAAAGCTTTAAAGACAAATAAGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GTTTTGCATCAGCGTGTAGA	GATTTGTAAAATCACCAGTCT	TTGAAAAGAATAATCGAGATCAGC	CTCTGTCAGCCTGCAAAGGA

 

Amplicon 5'

Amplicon 3'

GTTTTGCATCAGCGTGTAGAGTTCATCCTGGGCCGATGTGTAGCAATTGATGTCCTTGAGGCCACAAACGGGAGTGCCAAGAGGTTCTATACAGAAAATTCGATTAAATCGAGTTAATATTTTATCAATTCCAAAGTTCACTCACTGGGCATCCAGAATTTGGCACAACCGCATTTGGAAACCGTATAGTTGGCCAGACATTCGGTTTGGCAGTTGCTCTGGGAATAGGAACGGAAGAATCGTAGCGATCGCTCATCATCGAACAGACATTGGCGTCTAAATATGGATATAAATATGTTTTGTATCGCATAGTGTCATCCCTTTTAACACGAATCCACTCACTTTTCGGGTGTAATTTCGTGCAAATTGTCAGTGGACACGACATATGCTGGCAGCACACTGACCAGGACCTCATCGCCATGAGGGACTAGGATATAATTGCCCGTCGTCAGCGGCACACTCTCCGGGGAGTTGAGAAAGACCTGTCAAATTACACATTAGCTGCGCACCGGTCCACACCCAGTTCTAACCACACACCTTATAGCCCTGTTTGAAACTGCGACAATCGTAGTCGAAATTATGCTGCAAACCCTGCAGAAAGGCGAAGAACCCATTTTTCACCGATGAGAACACCGTCCGTTGGGGATACGCATTCTGACCCTGGTCCACGAATCCAGTGTCCAGTGACCAATTGCCAGTTATGTTGTTCCACGGAGGCAAATCCACATCGAAGTACTTGTTGAAGTCCACCACATCCGGATCCACATAGCTGATGAACTTCTCGTCACGATAAATGTCCTTGGGTCGCAGTCCATTGAATTGATAGCAGATACCCTCGTCGGTGGCCACAAAGTCGAAGATCTTGTCACAGAAGTAAAAGCGACCATTCCACTTGCAAAACGGACCCGTCTCATTCTTCGATATACTCAAGTCAATCAGAGTTTGGGTTACGTCCACGTTCGGCGGATTTAGCTGCGATAGCAATGGAGTAAACCGCTGCACCACTTCCGAATCACAGATGTGCATTGCAACCGCCATTCGAGCCAGGCTAAAGATATGGAAAATGATTGGTGTTAAGACTGGTGATTTTACAAATC

TTGAAAAGAATAATCGAGATCAGCAGATCATCTGTATATAAAATTTGTTAAATTACTAAGTGATCTAAGGGCCTACCCTTAACTATAAAAAGTATATGCTGAAATACTCAACTCCTCATCATCCAAATCGCTGATATTGCCATTCTGTTTGTACTCCTCCCAAAGTTGTCGCGATACGTTAGTATAGTCAAAGACATTGCGTTCCATTTTGATTTCCGGACAAATGGTAATTGTGGGAAACGGTATCTTATGCACTGGCACCAAAGTCTCGTCGAAACCTAGAATAACCGGCGATTCCTGCCACTTTAGATAGGTGTCCCAGATAAGTGAAACGGCAAAGTAAACGGAAATGCACGTGAAGAAAAGCCAATAAAGTCTGGAAGAAAATCAAAGTTATTCTATAGGTTCCTAAAGGGCTCTATTCTCTACAGACTTTTCATAGATCGGTCTGTGAACCTCGAAGATGTAGCGTATGCCGTGAATGGTCGTGCTCTTCGCATACTCCAAAAGTAATCCTTTGCAGGCTGACAGAG

 

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