• About
• Search
• MiMIC
  • Technique
  • Constructs
  • RMCE Lines
• Transposons
  • GDP
  • Others
• CRIMIC
  • Technique
  • Constructs
• Order
• Downloads

CRIMIC CR00623

Gene Tep3

CG7068

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2L:7705142..7705142	28C1	FBti0199430	78968

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	ATCATTCCCTTCCCTATCGACTCACC	GAGGGCATCCAAAAGGTATTGTCGCT

Images

Construct Design Sequences

sgRNA sequence	CTATTCATTCACGCGTCTAACGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TCGTTTAACACCCCATGGCA	ACAATACCCCAACCCGAAAG	GTCTTCCGTTAGACGCGTGA	TGCTCTTGTCCGTCTGAACC

 

Amplicon 5'

Amplicon 3'

TCGTTTAACACCCCATGGCAGAATCAATCAGGTGGAACATCGCTACTGCAACTGGAAACGTGAAGCCTGCATTTTGCGGACCATTTGAGCAAAAGCGAAATTGTTTTTGCCATTTACCTGTTGGTTGAAAACCGTTTTGCGAGAGCGACCACCATCGAGGGAACCTGTTTTTTTAGGCTAACAAACGTGACATAAACACAAAAGAAAATTTTTTGGTGGGAACAGGTTAAACCCGCCGCACCAATTGAAATTAGAACAGGCGGTAAGCAGTGAATTACCAGAGAAGGTGGCATAGTTAGGAGGCAGCTCATAAGTTTAGCAATTTAGCTGACAACTAAATGCATAGCCCGAGCATTTTAAAAAGGCAGCCAAATGTAAAAAGCACCAAAAAGGATAAAAAAAAGAAAAAGCTTACGTAAAAAACTAAAGTTACTCAAGCAACATAAGGCACCTTTCGAAAACAAGTTAGGTGTACGTTAGTTTGGCTGCAAAATAAATCCATTAGTTATTAGTTACAACTTTTAAATCCTGTTTTTTTTCATAAATTAACTGGTAATAAGGTAGCTTAAATATACCATGATAAATATATTCAAATAAGCCGAAAAATCTTTGATTAACAGCTGCTTATTGTATGGCTTAGCTGATTTAGGAAGCTTCCACATTCTGAATTTCGATGAGCCCACAAAAGAAAACCACACAAAAGTAGTTATAGCTCGAGCCACTCTGCCGTACTTCCCACAACCCCGACCCCAAGACCACAGACCACCAAACCACCTCCAACCATTTCCGACAGATACCTCACCGCAGCGATTGATGCTGACCCGACCGAAAGTTAAAGTACAATACCCTCTTTCGGGTTGGGGTATTGT

GTCTTCCGTTAGACGCGTGAATGAATAGCGAAAAAAAGATTCAGATAGCATAACAGCATAGGTGTAATCCACTTATCTACGAACATTAAATCTGTAGCTCTTTTTGTTTATTTATTTTACGGCAGTCGCCGGCTTGAGGTTAAGTCGGGTTTCGTTTGGAAAAAATGTTGTTCAAAATGTAAGGGGTATAGTAATAAGTTTAAAATCTTCACACATAGTTTTAAAGATATATTTCTCGTTTTCACTATTTTTGTAATAATTTAATTGTAGTTTATACTCCATCATTGCTCCGAATACTTTGCGTCCGAACTCGCAATTTCATGTGGCTGTGAGCTTGCACAATGCACCTGAATCTGCCACATTTAAGGTGGGAATCCTGGGGAGCTCTTACACGGACTTTAAGACCGTGGAGTTACGGCCCTTTTCCACACAGCTATTGCACTTTGAGGTAGGTGGTAACTATACATGATATAGCAAAAGACCTCCTATTTATCACAAATCAACTTTAGATTCCCGCCTTAAGAACTGACAGATATCGCCTAACTGCTGAGGGCCTTGGAGGAGTTCAGTTTACCAATGAGACTCAACTGCACTTTGAGTCCAAACAACATACGGTTTTGGTTCAGACGGACAAGAGCA

 

---

Search Again