CR00625

CRIMIC CR00625

Gene CG10505

CG10505

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	2R:21289894..21289913	57D8	FBti0200608	80608

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	ATTGCCTGCAATCGCTCATCTATCGC	TAGTCTCACTTTGTACGCAGTGGAGC

Images

Construct Design Sequences

sgRNA sequence	GCAAATGGCATGTCTGATCCAGG
sgRNA2 sequence

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GATGGCACCGATGATCTCGT	TTGCAAGTCGCCTACCTTGG	GGCATTACTACGTGACTGCC	TGCGCTATCGATTACGCCTT

Amplicon 5'	Amplicon 3'
GATGGCACCGATGATCTCGTTCATGAGCTTTACCCGTGCATCCCGATGCTTGGCGGATCGGGTGCCGAAACTAGCAGAAGCCTTGGCGGCCCAGGCTTGCAGGGGTATCAATATCACAATGAAGGCGATTCCAATCAGTGATGTCCAGCCCACCTGGCGGGTCATCAAGTAGCCAAAGATACAGGCCTCCACGGGACCCTTCCACAGGTCGTGAAAGAAGTAAAAAGTCAAGTCGAACTGCGAGAGATCGATGGACATCACCGAAATGGCCTGGCCTCCAAGTCCTTCGCCACTGCTCGCCGAGGCGCGCAGACATTTCCGGTAGACCAGGCCGGCACAGGCCAATCTGACCCGCGTTCCCACCGCGAATACGTAGAACATAAATGGGTGAAACACTAGGGAGGTGACCAGCGAGCACAGGACAATGCCCATGGCGTACAGATAGGCACTCTCCTTGGTCACCGTCGTCTGGCCGTAGGCGAAGTAGGAGATCAATCCTCCGAGCAGCAAAGGCTGGAAGGAACTGCAATTAAAGCGGAAGTGAAAAGTGAGATTGCTGTGATTTCCGGAAGTATATTTAGATTCTGTACTCACTGAATAGCCATTTCCAACAGAGAATATAAAATGCAAACGGGAACAAACTGCCATCCGTAGACCTTAAAGATCAGCCGCATAAGACTCGGATTTTTCTTCTTGGACTCCTTCTCCCAGTGGGGCTGTAGGTTTCTGGTCAAGGACTCACTATCGAAACTGGGCAGGTGTTCGTACAGCTTACTCGCGTCCAACTCCTCCCGCCTGCCCTTCCGAAAAACACTGTGCATCCATCTGAAGGAGGAAAAATGATAAGTCGTTCAATATCTTATCAAAGTATCTCCAAGGTAGGCGACTTGCAA	GGCATTACTACGTGACTGCCCAACAGTCAATGGTCAATCAATATCGCGAAATTCCGATTTATGACCGAAATCTCATGTGGAAACTATGGGTTAAAAATAACCATTATCACTTAATGAGTTTTGAAAAAGATATTCCAAAGCTGACTTCTGGGCATTACAAGTATCTTAACTTTTTTGCTTAAACTTTTCGGAGATGTTGTTTATCAGCACTTATTAGCAACAATTAGCACTATCCTTATCGCGAGTTGTAAACTATTCACTGATTGACCATGACCATCGTTTCCGCAGAGATTTTCACTTTTCACCAATAAAAAAAAAATATTGACGACTCACAGGAACAGCCACTTAGAGATTATATTGGCCTTGATAAACGGATTACTCTCTCTTTTCGGACTCGACTGCTTAGAAGCATCCATGGCTCCATATGCTCGCGGCGCCAGACGGATTGAATATGCCCCAAAACGGACGGTCGAAGGCGCTTTTAACGACTTCAGCACTCACAGACGGAGCAGTGACGTCTGTGGATTATTTGTCCGGAGAACCACCATCTTCTACGGTTTGCACTTGGCACAACGCCTGTCCAAACGGCGATAAAGTGGCCAACTCAACACGTTTTTTGAGAGTTCACCGCTTTTGATAGTGATACGTAGTGATAGTGACTAAGGCGTAATCGATAGCGCA

Amplicon 5'

Amplicon 3'

GATGGCACCGATGATCTCGTTCATGAGCTTTACCCGTGCATCCCGATGCTTGGCGGATCGGGTGCCGAAACTAGCAGAAGCCTTGGCGGCCCAGGCTTGCAGGGGTATCAATATCACAATGAAGGCGATTCCAATCAGTGATGTCCAGCCCACCTGGCGGGTCATCAAGTAGCCAAAGATACAGGCCTCCACGGGACCCTTCCACAGGTCGTGAAAGAAGTAAAAAGTCAAGTCGAACTGCGAGAGATCGATGGACATCACCGAAATGGCCTGGCCTCCAAGTCCTTCGCCACTGCTCGCCGAGGCGCGCAGACATTTCCGGTAGACCAGGCCGGCACAGGCCAATCTGACCCGCGTTCCCACCGCGAATACGTAGAACATAAATGGGTGAAACACTAGGGAGGTGACCAGCGAGCACAGGACAATGCCCATGGCGTACAGATAGGCACTCTCCTTGGTCACCGTCGTCTGGCCGTAGGCGAAGTAGGAGATCAATCCTCCGAGCAGCAAAGGCTGGAAGGAACTGCAATTAAAGCGGAAGTGAAAAGTGAGATTGCTGTGATTTCCGGAAGTATATTTAGATTCTGTACTCACTGAATAGCCATTTCCAACAGAGAATATAAAATGCAAACGGGAACAAACTGCCATCCGTAGACCTTAAAGATCAGCCGCATAAGACTCGGATTTTTCTTCTTGGACTCCTTCTCCCAGTGGGGCTGTAGGTTTCTGGTCAAGGACTCACTATCGAAACTGGGCAGGTGTTCGTACAGCTTACTCGCGTCCAACTCCTCCCGCCTGCCCTTCCGAAAAACACTGTGCATCCATCTGAAGGAGGAAAAATGATAAGTCGTTCAATATCTTATCAAAGTATCTCCAAGGTAGGCGACTTGCAA

GGCATTACTACGTGACTGCCCAACAGTCAATGGTCAATCAATATCGCGAAATTCCGATTTATGACCGAAATCTCATGTGGAAACTATGGGTTAAAAATAACCATTATCACTTAATGAGTTTTGAAAAAGATATTCCAAAGCTGACTTCTGGGCATTACAAGTATCTTAACTTTTTTGCTTAAACTTTTCGGAGATGTTGTTTATCAGCACTTATTAGCAACAATTAGCACTATCCTTATCGCGAGTTGTAAACTATTCACTGATTGACCATGACCATCGTTTCCGCAGAGATTTTCACTTTTCACCAATAAAAAAAAAATATTGACGACTCACAGGAACAGCCACTTAGAGATTATATTGGCCTTGATAAACGGATTACTCTCTCTTTTCGGACTCGACTGCTTAGAAGCATCCATGGCTCCATATGCTCGCGGCGCCAGACGGATTGAATATGCCCCAAAACGGACGGTCGAAGGCGCTTTTAACGACTTCAGCACTCACAGACGGAGCAGTGACGTCTGTGGATTATTTGTCCGGAGAACCACCATCTTCTACGGTTTGCACTTGGCACAACGCCTGTCCAAACGGCGATAAAGTGGCCAACTCAACACGTTTTTTGAGAGTTCACCGCTTTTGATAGTGATACGTAGTGATAGTGACTAAGGCGTAATCGATAGCGCA

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