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CRIMIC CR00631

Gene pros

CG17228

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:11390195..11390210	86E3	FBti0199432	78970

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	CCAAGAAACTTGTGGCGCTGGCATAT	TAATGCTTCGGTCGCTCGCTCTCTTT

Images

Construct Design Sequences

sgRNA sequence	GAGCCCAACCGAAACGGGGCGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGGTTTCTTTCGCCGCTTTT	GTTGGGCTCGGTTTGGTTTG	GTTGAGATCCTGGCAATCCCT	CATACTCTTAGGCGCGTCGT

 

Amplicon 5'

Amplicon 3'

GGGTTTCTTTCGCCGCTTTTCATGGCGGCAGCTACTTGAGAGTCTCGAGCACAATGGCCAGGGCCCTACTGGCAAAAGGCGCTCAAGGATACGCCCACACACATGTTAGCCCACTCACTTACACCCGCTCACACATACGCCCACACACTCGCACATCTCACGCTGTTCTTTTTCCGTGTTTTGTTTCTCATCGAGCCGCTTTCCGTTTTTTTGAGGTTAAGTGTCCTTTTGGCGCTTAGAGCAGCTTCCCAGCCATTCCCCACCACCCTCCACCTTCGACGCGTGAAAAGATCTTGCTGACAACAACAACAAGAACAACAACAACAACAAGAACAACAGCAGCAGCAGTTACAATTATATAAATACACACACTCACACATGCTACATACCACATTCAGACACTCAGATACGATACACGCTCCATTCTGTATATGTATATAGACGTATATGACTTGTACAGATGCTTATATATAGATACAATGCAAAAGTGTTGCCAATTACTTCATTAATGTATGAGGTAGAGATGTCTGCATATGTGTGGGTGAGTATGTGTGAGCTTATTAATAACATTCTACTCGTTCGTATTCGTAACCGTAATCCGTGATACGTGATTTATTGCGGAGGAAGTGAAGAAGAAGAAGAAGGTGCAGCGAAGAAGGAGACACACGCACACACGAACAAAAAATACACACACACACAGAAAACACACGCACCCACACTGTCACGCACACATTCAAACGAAGTGAGAGTGAGTTAGAGGGGTGCGGCATGGGTGAGAGATGAATGCACTTGACAAAGCAACGCCTGCCTGCCTTTCAACAAGTGTTTTAGTGGTTTTTCTTCTCACTCTGCCACGCTCTCGCCTCACTCTCTCTCTCTCTCTCTCTTCGTGCGTGTGTGTGGGTGAAGGCCCAAATAAATAGGCTACGAAATATTATATGCAAACTAATTAGCACTTCCGTCGAGCCCCAACGCCATTGCATCTGCACATCTGCAGTTCCACTTGCACGGCTTATTATCTAAATTAAGTGGCGCATTTGAAACCGCCAAGCCAAGCTTCCTTTTCCTGCCACATATGGTTGCTTGCATAAGGATCGCCTCAAGCTCCTTTTCACGCCAGCCACAAAGCTAATTGCAATTCAAACCAAACCGAGCCCAAC

GTTGAGATCCTGGCAATCCCTAATAACTATGGACACCCGGCACAATGAACTGTCACACAAAACTGTCACACAGCGATTCCTTTTCTTTCGGCCAGCCCAAGCCATCAGCCTCATTATGAAGCCATCCCAAAACCACCCACCCGATCCACATCCCCATTCCAACGCAGCACCTCCCTCCTGCTCTTCAAGTGCCAGCGCCTCCGGGGAGCCGCAAGGGGGGCGTGCCAATGGGCTGGGGGCCAGAAGGAAAAGATTTTCTATTTGCGCATTCTGCGCTTATTGTTTGGAAAATCAACACGAGCGCCGTAGCACAACATGAGAAATGCATTTGGCGCTGCTACTGGGAAATCTTGAAATTAGCGCGATTATCGGAGCGTTGTCAAGAGGGGTCGATGCGGGAGGGTTGGGGCGAGGGACTCCAAAGGATTCCCAGCGAAAAGACAACAATTAGTGCGCGGCAGACAATGCAGGAACTGCGAGAAGAGAGGAGAATCAAGGGGTTGGAGGAGGCGGAGCAGCAGCAGCAGCAGCAGCAGAGGCAGTCAGGCCGGAAGACAAGCAATCACTTACAGTTGCCAAGTTAAGCATATACCCCTAGTCCTATCCCCCTGCCACATCCTCGCCATATCCCCTTGCGAATGGCAGCTCACACACAAATCACTTGACTAAACAAGGCGACGCAGCAGCAGCACCAGTAGCAGCAACTGCGGCAGCATCGCGCTGCACTTGCTAATTAAATCCCCAACCCCCAAGGCAGTCCTGGGATAAAAAAAAAATAGGAAAGAAGGCAAAAACCGCAGCTAGAAGATGCCGCCCCGACTTGCAATTGTTATTGCACCACCACCAGCTGCTGCTGCACATTTGAGATTTATTAAAAATTCTTTACACACCCACACACACCCCCACACCCACAGCGACACCGACACCGACATATACACGACGCGCCTAAGAGTATG

 

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