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CRIMIC CR00633

Gene Mrp4

CG14709

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:11570082..11570085	86E11	FBti0200609	80609

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	TAATGAGACCGTGCAACAGCTGTTGC	GGCAAGCAGGAATACTCCCGTAATGA

Images

Construct Design Sequences

sgRNA sequence	ACACCTAAACTAGTCATCAATGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
GGATCGGGTCAGAACGAGTC	TTTTCTTATCATCGCGCCGA	GCACCCATTGATGACTAGTTTAG	CTTCACGTTGGATCGGCAAC

 

Amplicon 5'

Amplicon 3'

GGATCGGGTCAGAACGAGTCGGACCTTCGGAGCGAGCAGATAGTCGTGGGTAGAGTAGCGCGCAGTGCCATGTGTACTTCGATACGTTCGCGATAAGTAAGATGTGCTATGAACTAAATCACATACGAAATACAAGGCAATCTATGACGAAATACGCCTGCAACAGATAGAAATACCGAATATCTCCGCGTGAAGTGATCTTTTTTGGAACCGTGATCTCGCAGCAGCACTTCTTCCAATCCGAATGCCACGCAATATTGAAGTCTGCATTGTGCCAGTGGAAAAGTTTACTTCCCGCAAACGTGAATAGAAATCGAATTCGTAGATCGGAATCTGAAATCGCTCGCATTTCCATCTGGTTTCTCCTCGGCTAATGCCAAACTAATTATATTAAAATAGAGCGCGTGTCCCCTTTCGTATCGAAAAGTGCTAATCAACCGCCGTCGTCCAAAGGCAATCCCGGTGACCGAGCAAGTCCCCTGTACTTGTAATGCTACACTCTATATAGTGCAATTTAGCTCCAATCAGTGCAATCAGCATGCAGTCGATGAAAGCCGATGAACTGCCGGAGAATCCACGGGAGCGATCCAGTCCCCTCTCGTCACTTATGTTCTGGTGAGCGTAGAAATTATATCTTTCTGGGCATTTCGAGACTAAATTTTTATTTCTTTTTAATTAAATGGTTTGTCCAACCATCTCATTGCAAAAAGAATAGAATATATATATGTATATGTATGTGGGTTATAAACTTAATAGCTCTAATAGAAACCAGTTTACAAGTCTATTAATACACATATTCAAAGATCATGGCATTGTTAGTAAACTAAAATCGAACATTTTTTTTCGGCGCGATGATAAGAAAA

GCACCCATTGATGACTAGTTTAGGTGTTATTTATTCATGTGCTTTGTTGTGTGTGTGTGTGTGGGGGGGGGGGATTTGGGGAGCATATAACGTGACAACACTGACAAGTTGATTATTTCATTCAATGGTTCGCTTAATTCGCGTGACCGAGGCGACAAAATCATTTTGCATTTCACGGGAGCTCGGATTCCCATTCAATTGGTTACCAATTGAGGGCGACACTTAGCTAAGATCCATTTTCAAAATTATTCCAGAGGAAATCTAACAGCGCTAGGTACAGAACTAGATATCCAAAGGGGAAAAACTGCTGACAAATGTTATGAATCATTGCGATAGAACACATAAACTTAAAACGCAACAAACTAATTTTGATGTACAGCTTCTGATAAGATTCCTTTTTGACATAACAGAATTTGTTTATCTTCGGCTAACGTATAGAGCTTTTATTTTTTGTTTATGTAATGTAATTATTTAATAACATATAAGATAAGGAACTCTGAACTGTTCTTTTGTGTCCGCGTATCGTAAGATATAGATTCATTTGCGTCAGCAAATATGGATGCTGTGAAAATGTACATACATGTACTCTTTTATTAATAAAACATTCGGTTTTACTGAGATAAGTACGGTAGTTATTAAAATTGAACTTTGTATGGGTTTTGCGCAGTTGCATAGAAGTTTAATTATTATTTCTGTAGTCTGTGAAATTTGAGCTTTGAGGAAAAAACATCATTCGCTTCTAACAATTTTGACCGTGACATACTCGCTTTACAACGCTGCATTGGATAGCAACTGTTGTCAGTTGTTGTCAATTGTCTGTTCGTTCGTTTTTTACTTTATCAGTATCATTGCCTGATAACAGACAGTCTTTCGCTAATTATGTTTTTATTGTACACCCAATAAAAACTACCTACGTAATGCTGTTGCCGATCCAACGTGAAG

 

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