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CRIMIC CR00634

Gene CG6574

CG6574

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	3R:10862153..10862165	86C7	FBti0200610	80610

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	no band	TAGTTGTTAGTGCCGACCAGGTGAGA	TTTCCTCCTCCAGATTTCTGGCCACT

Images

Construct Design Sequences

sgRNA sequence	CGAAGGCAAAAGTTTGACTTTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
ATCAGAAGAGATGCTCGCGG	TTGCCTTCGCTAGCCAAAAA	TGGAGTAGGTCACCTTCTGG	TGAAGTGCGACACGTGTGTA

 

Amplicon 5'

Amplicon 3'

ATCAGAAGAGATGCTCGCGGTCTGAGCCTTCCGGTGTGCAGATAACCAGCGGCCAAAGCACTTAGAGCAGCCAGAGCTGTCAGGACAGCATCAACGGCGCCGTTCCACGCAATAACAGGCTCCGGTTCGATCACTTTCCACAGAACCTGAACGTAGTAAGTGACCTGCAGGTAACCAGCCAGTCCAATGGCGTACCACAGACTCCACTGAACGACTCGCGGATTGGTGTATGCATTCCGAAAATGTAGCCAAAGAAGGCTCAGTGCTCCTGGCTGCTTCTGAGTATTCTCCTGCTGCTGATCCATCTCAGCCAGGTTTCCCACCCCGGGTAGCTGAGTCTGCGGCTCCGTAATGGACAACTGCTCCCGATGAAAGTAAAGACTCCTATCCACGCGGGGCAGGCCAAAGGCCCAAAGCATGGCTATAATCTGAGCTGTAGAATATATGTGCATGATTAATGCCCCAAGTTGTAGGTCATTTATTGTACGTACTGCCCAGCGTTATATAGTTAAGCTCCTTGTAGTTCATCAGCTCCAGATTGAGCATAAGTTGGGAAGTGATGCCGGAAACCAGTTTGCCTGCAAACATGGCTGCTCGGGTGTGGGAGGTCACTCTGGCATAGTATTTCTTGTCCACTTTGGCATAGATGTACGTATAGTAGGCCACCTCTGCGGCCATGTAAGTGCCGAAGAAGAACTCCAGGATCTGAAGCGACAGCAAGCTGGTGGTCCAGATGAGCATGCTCCATATTATCACACCCGCAGCTCCAATTGTGATGATCAGCGGCTTGTATCTGAAACATAATACATATAGTTATTATAAAATGAAGCCTGTTTCCGTTCGAACAGCTTTTAATTGCAAAGCTATCAAATCGAATTCAATTTGCTGAATCACAATAAATTTTTGGCTAGCGAAGGCAA

TGGAGTAGGTCACCTTCTGGTTACAAATAAAATGACTGGTTTCGTATTTGATTACGAACAATTTTTGTTATAAAATATAAATATCAAATTGGCTGATAGCAACTCAGTCCCACGTTTAATTTAAGAAAATACATTTTATCCTTAATTTAAAAGATAAATAATTTTCAGCGAATAGTTACCTGAGAAAATCTGTTATGAGAAATACAAAGACCAACTGCACCAGGTAGGAATAGGTTCCCACTGGGTAAACATCGTGCGTTAGCTGGAATGGGAAGAAATTTGATATTCTATGGAATGGATAGATGGTGGGAGATGCCACTCACCTGCTGCTCCGTGATATTCCGCCAGGGACCCAGCAGGTACTCCGTGACGTAGGGCTCCGACGGACGGATCTCGCGGATGCAGCCAAAGACACAGAGGAGGCAGGAGATCTTCAGCCACTCCTTCATTTTCGAAATTTCGTAACGGTCACTGACGTAACGTGCGCTACGCAACGAGATTCAGCGGCGCAAGCTCTTACGTGAAACACTGAAGTGGGATGCCGTTAGCTGGGCCTTTCTGCATCTGGACAGGTGAAGCTCAATATATTGCCTAGATTCACGGGCAATCTGGTGTATCAGGCCATGGTCGTCGTCGCCGGGTGCTCAAATCTAATCTCTTCGCGACGCGAATCCGAAATTATCACTTCGACGTGCCCGAATTGTATTTACACTGAAATCGCTGTTAGATAATTGCGAATTTACAATGCGGTTTGAAAGTCACAAGCAAACAATCGCATGTGGAACGCACAGGTGGGTGGTCCAGTTTAAAAGTTCTCCAGTCACCCTACACACGTGTCGCACTTCA

 

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