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CRIMIC CR00636

Gene CG9657

CG9657

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	X:7419709..7419709	7B2	FBti0199433	78971

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
OK	OK	GGCAACAGCACAATTGCACTTCCATC	TTTGTCCCTCGGTCAGTGGACTCTTT

Images

Construct Design Sequences

sgRNA sequence	AATCAAATGGAATTTCTTCCTGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
AACTGCGCTTAGAGTCCAGG	CTGGGTAGAGTCGCCTTGA	CTCTACCCAGGAAGAAATTCCA	CGAAGCGTGGGTAGCGATAA

 

Amplicon 5'

Amplicon 3'

AACTGCGCTTAGAGTCCAGGAAACAAAAGGCCAATGGCTCAGAGGAGGCGGCGAGTAGTGCTGCCTCGTATTTGGTTGGTGGCAGGCAGATGAAGATCTTTCCCATAACCATGTCACTGATATCCAGGTGAGGAATAGGTTAGAATTCTACGATAAATCATTTTTAAAGATATTTTTTATTTTATTTTCCCTTTAGGGGGAGTTAAGTAACTTTTATATCCAAAATAAATATGATAGAAACGTTTAATGAAACTATAAACTTAATTTTTTCAACTATAGCCATTTGCTAAGTATATTATAAAGTCGAGATGGAAAGCCCCATTCTCTTCTCACTGTTAAGTGCTTAATGTGCCGGAGATAAGATAGAATTAATCAAAGTACCGCGGAAAACCCCATTTGTCCTCAACTAGAGAGCTTGTCTGAAGATTCATACAGCCATACAGGGAAAAAAATGCCGACATAGTGTAGGTTGTAGTTTGCTCCAGTTTTCGACCCGTTATAGTCGCTCGCTTATTTTTAGCATACTTGTACAGCTCGCTCCTATCAACGCACCAAATCAAAATGTTTCATAATTAATAATTGGCTTCCACGTTCGCTGACAATTCGAGCTACCCGCATATGCAGGGCATTCAAATTGGCGACGGTCAATTGGCGACTACGACTGCGAGTCCACTTCCACTTCCACTTCCCCGAACCCCAAATGCCCGATCTCCAAGTGGCAACTAAGAAATGTATGGAAACTTGACTTGGACACCAGAGAATTACGACGACATTGGTGCTAATTGAATTGTATAAAACGCACTTTGATCTCTTACAGATGATGGATGATCGGTGTAAGGCCTTTGTCGAAGGTCAAGGCGACTCTACCCAG

CTCTACCCAGGAAGAAATTCCATTTGATTAAGCTTAAGTTTTAATATCTTTACTAAGATCACATTTTGCTTTTCAGCTTTATATCTGGAATTACACTTTTGGGTACTCCCACAGAGGTTTATCTTTATGGTGCTCAGTACATGTACATTATGGGATCGCTGGTTTTAATGGGCTTCTGCATGTACTACTTCTTCTTGCCGGTCTTTCACGAACTCAATTTGATATCCACTTATAAGGTAAGCTTATATCTTGTTTGTATAACGAATCAATTTGAATGGGAGGATTTTATTTTAGTATTTGGAACAGCGATATAACCGAAGTTTAAGGCTTTTCGGATCTGTCATGTTTATTGTGGCTTCGGTGAGTTAATCTCAATTATAAGTAGGACTCTCTTTTATAATTTCTTTTTTTTTCTTTCCAAGCTACTTTGGCTTCCAATTGTGATTTATGTACCGGCTATAGCATTCAATCAGGCCACTGGAGTGAACATCCATATAGTGACGCCCATTGTGTGTGTGGTTTGCATTTTCTATACTTGCATTGGAGGTTTGAAGGCGGTGGTTTGGACGGATGTCATCCAAACGTTGATCATGTTCGGTGCGATGGCTTTGGTTTTGATTAAGGGAACCCTGGATATCGGCGGACCTGGAGTTGTGTGGCAGAAGGCCCAAGAAACAGCTCGATTGGAAGTACCCAATTTTAGTGCGGATCTAAGTGAGCGGTATACCTTTTATTCCCTGGTTTTGGGCGGTGTAGCTCATTGGCTCAAGTCAAATGCAATTAGTCAGAATATGATCCAGAGATATTTATCGCTACCCACGCTTCG

 

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