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CRIMIC CR00639

Gene CG4829

CG4829

Information

Donor	Location	Cytology	FBti	Stock number
pM37-p2	X:16824828..16824828	15A11	FBti0200612	80612

PCR Verification

PCR check relative to sgRNA sequence

HA-L	HA-R	Forward	Reverse
no band	OK	CGATTCATCTTAACCAAGCACCTGGC	ATGCAATGTTTCCGTGCGTGTGTGTG

Images

Construct Design Sequences

sgRNA sequence	AAAGTGCACTCAGAGAAATGGGG
sgRNA2 sequence

 

Amplicon PCR 5' Left	Amplicon PCR 5' Right	Amplicon PCR 3' Left	Amplicon PCR 3' Right
TGCACCATCAGGATTAGCAA	GGGGCGTTCTTTTAGAC	ACGCCCCATTTCTCTGAGTG	CCCCGTTTGGTCAGCAATTG

 

Amplicon 5'

Amplicon 3'

TGCACCATCAGGATTAGCAACAACAATAAGCACCAGCACAACAACAATAGCAACAAGTGCAACACAACAACCACCAACAGCAACAACAACAACAACAGCAACCAGAACAACAACCACTAGCAGAACAGATAGCAATCCCAGCCCCACACCTAAAACATTTCATTTAACAGATGATGAGCAGCAACAGGAACCGGAAACGGAAACGGACAACATTGGCAATCTCAATCTGGGAATCACGAGCACAGCGACCACGCCATCGGACGACGTCTTGCCCAATGTGGCCGCCTCCACGGCGGCAACGACAGCGGCACCACCCACCTGGCTAGCGGATCAGATCAAGATCGATACCAGCGAACAGTACAACTCGATCCTGGGGGTCTACCAACATGGAGCCGTGAGTTCCGATAACCTTGAGTGCAGCAAGATCGGCAGGTAACTATATGCAGTTCTATGGGCTTTAAATTTAGAATTTAAAAAGAATTTAGATCGAATAGTGAAGGTAGAGGAATATGGGAATGAGCCACCTTTTGCCCAAACGATCGAGGTCATCAAAATTTCATTTTAGGATTATTACTAACACAATAAGAAGCGTTATACAATTTTTGGATCACCTAACAGAAAGACAGCAGTGACATTAAATATTAAATATTAATTGAATATTATTATTATTAGCGGCATCCTGCAGAAGAATGGCAGCGCGGTGGACGCAGCGATCGCCGCCCTTCTGTGCAACGGACTGCTGACCCTGCAGAGCATGGGCATCGGTGGTGGTCACCTGATGAACGTCTATAACCGAAAGGAGCGCCACGCCACCGCCATCGATGCCCGCGAGGTGGCGCCCTACGAGGCCACCGAGGACATGTTTGCCCAGCAGCCGGAGAATTCCGACAAGGGACCGCTGAGCATCGCTGTGCCCGGCGAGGTGATGGGCTACCATGTTGCGCATCAGAAGTTCGGACAGCTGCCGTGGGCGGAACTGGTGGCGCCATCGCTGGAGCTCTGCGAGAAGGGATACCATGTGTCGCAGCACATGGAACGGGCACTGGATATAGCTTTGCCGTACATCAAGGAGCACGAGCAGTACCAGTAAATTGTCGAAAAGCTTGAAAAGTGCTCTAAAAGTGTCTAAAAGAACGCCCC

ACGCCCCATTTCTCTGAGTGCACTTTTCAGCAGCTTTTCAAGCAATTTCAAACTCATACTTTTGTGAATAGAACTCGATTCTAAGCGTATGATTCTTTACAGAATCTATTTGAATGCGGAAACTGGCAAGCCCCATGTCGCCGGAACTGTAGTTAAGCCGCCCAAAAATCTCTGCAAGACCTACAAACTTCTGGCCGAGAATGGACCCATGGACATGTACAATGGAACGATAGCCAAGTTGCTGGCGGAGGATCTGCAGGACATGGGCAGTATTATTTTGGCCGATGATCTGGAGTCGTACGAGGCCGATGTAATCAACTCCATCACCATGGACTTGGGTGACGACACCTTGTATGTGGTGCCGCCGGTCAGTTCCGGTTCGGTGGTGGCTCACGTCCTGGCCATACTGCAGGGCTATAACTTCACGGCACAGGATCTGTCCACGGAGGAGCTGAAGGCGCGTACCATTCATCGATTTGCCGAAGCCATGAAGTTCGCCTTTGCCCAGCGTCCAGAATTGGGCGATATGCATTTCATCGATGCACGAGAGCTGGTTAGCCGGCTGAATAGTCCGGACTTTGGCGATCAGAACCGGGCCAAGATCAATGATTCCCACGTGCTGCCCGATGCCCAGGCGTATGGAGCCAACTTCGCCGCCATCGAGGATCTGGATGGTACTTCGAATTTGGTTGTCTTGGCGCCCAACGGTGATGCCGTCTCAGTGACCAGCTCCATCAATTCCTAGTGAGTATCCATTGTTAGCCTCCGTGAGGTTTAAAAATAATCTGGATTAATCCATTCAATATTTATCCATCAGCTTTGGCTCCGGATTAATTGGACCCCGTACGGGTATTGTTCTCAACAATGGAATGAACGATTTTGCGGTGAAGAATAACATTTTCGCATTGCCCCAGTCGCAGTCGAATAAAATTGAGGCCCACAAGCGGGCGATGTCCTCGCAATCACCGATCCTTCTGGCCGATAAGGATGGCAACATGAAGATGGTCATCGGAGCAGCTGGAGGCAGCAAGATCATTCCGGCCGTGGTTGAGGTGGCCGCCAATGTTTTGTGGTTCGGCAAGGATCTACGCCAGGCGGTTGATGCACCGCGCTTCTACCATCAACTGATGCCCGATGTCCTGGAGTACGAGGAGGATGGCTTCACGGAATCCCTGCTCCAATTGCTGACCAAACGGGG

 

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